pGBKT7-Ppp1cc plasmid

PVT93322

pGBKT7-Ppp1cc plasmid Information

Plasmid name: pGBKT7-Ppp1cc(mouse)
Alias: NM_001424537.1;dis2m1; PP-1G; PP1
Gene lenght: 1011bp
Host: Yeast
Use(s): hybridization
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Kan
Screening marker: TRP1
Fluorescent labeling:
Promoter: ADH1
Replicon: 2U
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: High
Induction:

pGBKT7-Ppp1cc plasmid Description

pGBKT7-Ppp1cc is a plasmid suitable for hybridization in yeast.

pGBKT7-Ppp1cc plasmid Sequence

1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg 61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg 121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac 181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga 241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt 301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct 361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac 421 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 481 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 541 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt 601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca 661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa 721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta 781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga 841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt 901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc 961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac 1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg 1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa 1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat 1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc 1261 tgatctcaga ggaggacctg catatggcca tggaggccga attcatggcg gatatcgaca 1321 aactcaacat cgacagcatc atccaacggc tgctggaagt gagagggtcc aagccaggca 1381 agaatgtcca gctccaggag aacgagatcc gaggactctg cctgaagtct cgggagatct 1441 tcctcagtca gcctatcctt ttagaacttg aagcaccact caagatatgt ggtgacatcc 1501 acgggcagta ctatgatttg ctccgtctgt ttgaatacgg tggctttcct ccagagagca 1561 actatttgtt tctcggggac tatgtggaca ggggcaagca gtccctggag acaatctgcc 1621 tcttgctggc ctacaaaatc aagtatccgg agaacttctt tcttctcaga gggaaccacg 1681 agtgcgccag catcaatagg atctacggat tttatgatga gtgtaaaaga agatacaaca 1741 ttaagctgtg gaaaacgttc acagactgtt ttaactgctt gccgatagca gccatcgtgg 1801 acgagaagat attctgctgt catggaggtt tatcaccaga tcttcaatct atggagcaga 1861 ttcggcgaat tatgagacca actgatgtac cagatcaagg tcttctttgt gatcttttgt 1921 ggtctgaccc cgataaagat gtcttaggct ggggtgaaaa tgacagagga gtgtccttca 1981 catttggtgc agaagtggtt gcaaaatttc tccataagca tgatttggat cttatatgta 2041 gagcccatca ggtggttgaa gatggctatg agttttttgc aaagaggcag ttagtcactc 2101 tgttttctgc acccaactac tgtggcgagt ttgacaatgc aggcgccatg atgagtgtgg 2161 atgagaccct catgtgttcc ttccagattt taaagcctgc agagaaaaag aagcccaacg 2221 ccacgagacc tgtcacacca ccacgggttg gatcaggcct gaacccgtcc attcagaaag 2281 cttcaaatta tagaaacaac actgtcctat acgagtgagg atccgtcgac ctgcagcggc 2341 cgcataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgcgcgc 2401 ttgcagccaa gctaattccg ggcgaatttc ttatgattta tgatttttat tattaaataa 2461 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa 2521 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagcatgag 2581 gtcgctctta ttgaccacac ctctaccggc atgcaagctt ggcgtaatca tggtcatagc 2641 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 2701 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 2761 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 2821 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 2881 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 2941 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 3001 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 3061 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 3121 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 3181 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 3241 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 3301 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 3361 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 3421 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 3481 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 3541 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 3601 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 3661 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 3721 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac 3781 ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg 3841 aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc 3901 tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg 3961 acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg 4021 gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca 4081 tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca 4141 acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt 4201 ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa 4261 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca 4321 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc 4381 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat 4441 attcggcaag caggcatcgc catgagtcac gacgagatcc tcgccgtcgg gcatgctcgc 4501 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc 4561 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg 4621 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat 4681 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc 4741 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg 4801 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc 4861 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac 4921 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac 4981 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatactct tcctttttca 5041 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 5101 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaacg 5161 aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa 5221 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg ctattttacc 5281 aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt 5341 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga gagcgctatt 5401 ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc gagagcgcta 5461 tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct ataatgcagt 5521 ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc 5581 tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga ttactagcga 5641 agctgcgggt gcattttttc aagataaagg catccccgat tatattctat accgatgtgg 5701 attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt ggtcagaaaa 5761 ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg tttacatttt 5821 cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg tctaaagagt 5881 aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg 5941 aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca aagagatact 6001 tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt acagtccggt 6061 gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg 6121 aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaaa gcgtttccga 6181 aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact gttcacgtcg 6241 cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc atagtgcgtg 6301 tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg tagtctagta 6361 cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca gcactaccct 6421 ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc aatgctatca 6481 tttcctttga tattggatca tactaagaaa ccattattat catgacatta acctataaaa 6541 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 6601 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 6661 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 6721 catcagagca gattgtactg agagtgcacc atagatcaac gacattacta tatatataat 6781 ataggaagca tttaatagaa cagcatcgta atatatgtgt actttgcagt tatgacgcca 6841 gatggcagta gtggaagata ttctttattg aaaaatagct tgtcacctta cgtacaatct 6901 tgatccggag cttttctttt tttgccgatt aagaattaat tcggtcgaaa aaagaaaagg 6961 agagggccaa gagggagggc attggtgact attgagcacg tgagtatacg tgattaagca 7021 cacaaaggca gcttggagta tgtctgttat taatttcaca ggtagttctg gtccattggt 7081 gaaagtttgc ggcttgcaga gcacagaggc cgcagaatgt gctctagatt ccgatgctga 7141 cttgctgggt attatatgtg tgcccaatag aaagagaaca attgacccgg ttattgcaag 7201 gaaaatttca agtcttgtaa aagcatataa aaatagttca ggcactccga aatacttggt 7261 tggcgtgttt cgtaatcaac ctaaggagga tgttttggct ctggtcaatg attacggcat 7321 tgatatcgtc caactgcatg gagatgagtc gtggcaagaa taccaagagt tcctcggttt 7381 gccagttatt aaaagactcg tatttccaaa agactgcaac atactactca gtgcagcttc 7441 acagaaacct cattcgttta ttcccttgtt tgattcagaa gcaggtggga caggtgaact 7501 tttggattgg aactcgattt ctgactgggt tggaaggcaa gagagccccg aaagcttaca 7561 ttttatgtta gctggtggac tgacgccaga aaatgttggt gatgcgctta gattaaatgg 7621 cgttattggt gttgatgtaa gcggaggtgt ggagacaaat ggtgtaaaag actctaacaa 7681 aatagcaaat ttcgtcaaaa atgctaagaa ataggttatt actgagtagt atttatttaa 7741 gtattgtttg tgcacttgcc gatctatgcg gtgtgaaata ccgcacagat gcgtaaggag 7801 aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt 7861 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca 7921 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta 7981 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta 8041 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg 8101 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga 8161 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg 8221 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc 8281 cattcaggct gcgcaactgt tgggaagggc gat

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???