pGL4.11-IL17A-Promoter-Fluc plasmid

PVT102146

pGL4.11-IL17A-Promoter-Fluc plasmid Information

Plasmid name: pGL4.11-IL17A(human)-Promoter-Fluc
Alias: -
Gene lenght: 2001
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: IL17A
Replicon:
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: RVP3
Reverse primer:
Copy number: High
Induction:

pGL4.11-IL17A-Promoter-Fluc plasmid Description

pGL4.11-IL17A-Promoter-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pGL4.11-IL17A-Promoter-Fluc plasmid Sequence

1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCGAGGATGG GGAAGGGCAT TTCAGAAACA 61 ATACCTTAAC AAAGGGTGGG TAAGCTTTGA AAGAAGGCCT GGAGAAAAAT CAGGACCCCA 121 TCATCCCAGG TCCTGGAGCA TGGTGGGGGG TAAGGCTAGA AAGGAAGTTG TACGAACTCA 181 AATATTAATT CAAATGCTAA GAGGCTTACC CTTCATTCTG TATGCAAGCT AATGGCAGAA 241 GAAAAGGCAC AATTAGAGCC ATGCTTTGAG AATCATTATT GAAAGCATGT CGAAGATGGT 301 CTGAAGGAAG CAATTGGGAA AAGCAAGCAT AGCTCATCCA AGTGGGTGAG AGTGTGAGTT 361 AGAGGAAGCT TGGAAATTGG TGATGTGAGA GATGCTGCAG CTTCTGGGAT TGCTGCCTGG 421 TCGTGTGTAG AGGAGGGGCA GTAGGGCTCA TTCTGAATCT TGTCTTGAAA AGCACATAGA 481 TAGTGATGCC AAAACCAGGA CTACGGAAAT CACTTGAAGC TGTATCCTAC CTCCTCCTCC 541 ATCTGTATCT GCTTCACCTA TCAAGGATAT CTACTATTGC CACTAAAATT CAGGTGCTTA 601 TGGCCTCCCT CATTCATCAG CCAGGGTTTA TCTGGCCAGG AAAGAGAAGC CCCTTCAGGC 661 ATTTGCAACA GAGGGAGTTT AAGTCAGAGA ACTAGTCACC CTGGTAGTTG AGATTGCCAT 721 CAGCAAGAAG CTGTTACCAT TTGAAGGCTG CAGGGACAAA GGGAGTGAGC AGTCCTCTGG 781 GAGACTGAGG AAGGAAGCTC CTGGCTTCTC CCCACTTTCC ACTTTCCACT TCCCACTTTT 841 ACTCATCTGT CCTCCAATTC CCTTTTGGCT GAGCCTAGCT GAAACCCAGC TGACAGGGGA 901 GTTTGAGCAA GCAGCCTCCA GGGTCAGCCC TCTGAGTTAC AGGTAGAGCA GGACAGGGAG 961 GAATGGATCT CAGGACAAAC AGGTTCAGGA TCCGGCAAAC TAATTTTACA CTCCAGCCAT 1021 TGAGTTGGAA CTACTGGCCA GCCTCCCCCC GAGTTAGCAT GTAGAATATG GGATACCAGC 1081 TGAGTGCCTG AGAGTTATCA TTCACCTCAG TGGGGGTAGG GGCGGAGAAG GGTGACATAT 1141 AGCCAGCCAC ATCTATATCC ACTGGCCCTT CCTTGTCCTA GTCCTCTGTA TTCCTGAGAA 1201 GGAACTATTC TCAAGGACCT GAGTCCAAGT TCATCTTACT TAGAGTACAG AGAAAAGAAC 1261 CGCTAACTCC TTCTCTCTTT CCCCCATCAT GTCTCCTCTC CTTTCTAGTT CTCATCACTC 1321 TCTACTCCCC CCTGCCCCCC TTTTCTCCAT CTCCATCACC TTTGTCCAGT CTCTATCCCC 1381 ATTTTCAATT CCTTCCTCAA AACACCAAGT TGCTTGGTAG CATGCAGGGT TGGAACATGC 1441 CTTTAACAGA AAATCTCGTG TCTCTTGAAC CTAGTTATTT ATTCCTTGAG CAGAGTAGAT 1501 ATTCAACAAA AGAATTGTTA AATTCAATTA AATAGGATAT ATCTTATTAT TAAATATTTT 1561 TTTCATTTTT TGTTTACTTA TATGATGGGA ACTTGAGTAG TTTCCGGAAT TGTCTCCACA 1621 ACACCTGGCC AAGGAATCTG TGAGGAAAAG AAAGATCAAA TGGAAAATCA AGGTACATGA 1681 CACCAGAAGA CCTACATGTT ACTTCAAACT TTTTCTTCCT CATGAACCAT TAAAATAGAG 1741 CATAACTCTT CTGGCAGCTG TACATATGTT CATAAATACA TGATATTGAC CCATAGCATA 1801 GCAGCTCTGC TCAGCTTCTA ACAAGTAAGA ATGAAAAGAG GACATGGTCT TTAGGAACAT 1861 GAATTTCTGC CCTTCCCATT TTCCTTCAGA AGGAGAGATT CTTCTATGAC CTCATTGGGG 1921 GCGGAAATTT TAACCAAAAT GGTGTCACCC CTGAACCCAC TGCGACACGC CACGTAAGTG 1981 ACCACAGAAG GAGAAAAGCC CTATAAAAAG AGAGACGATA GCGCTACATT TTGAAGCTTG 2041 GCAATCCGGT ACTGTTGGTA AAGCCACCAT GGAAGATGCC AAAAACATTA AGAAGGGCCC 2101 AGCGCCATTC TACCCACTCG AAGACGGGAC CGCCGGCGAG CAGCTGCACA AAGCCATGAA 2161 GCGCTACGCC CTGGTGCCCG GCACCATCGC CTTTACCGAC GCACATATCG AGGTGGACAT 2221 TACCTACGCC GAGTACTTCG AGATGAGCGT TCGGCTGGCA GAAGCTATGA AGCGCTATGG 2281 GCTGAATACA AACCATCGGA TCGTGGTGTG CAGCGAGAAT AGCTTGCAGT TCTTCATGCC 2341 CGTGTTGGGT GCCCTGTTCA TCGGTGTGGC TGTGGCCCCA GCTAACGACA TCTACAACGA 2401 GCGCGAGCTG CTGAACAGCA TGGGCATCAG CCAGCCCACC GTCGTATTCG TGAGCAAGAA 2461 AGGGCTGCAA AAGATCCTCA ACGTGCAAAA GAAGCTACCG ATCATACAAA AGATCATCAT 2521 CATGGATAGC AAGACCGACT ACCAGGGCTT CCAAAGCATG TACACCTTCG TGACTTCCCA 2581 TTTGCCACCC GGCTTCAACG AGTACGACTT CGTGCCCGAG AGCTTCGACC GGGACAAAAC 2641 CATCGCCCTG ATCATGAACA GTAGTGGCAG TACCGGATTG CCCAAGGGCG TAGCCCTACC 2701 GCACCGCACC GCTTGTGTCC GATTCAGTCA TGCCCGCGAC CCCATCTTCG GCAACCAGAT 2761 CATCCCCGAC ACCGCTATCC TCAGCGTGGT GCCATTTCAC CACGGCTTCG GCATGTTCAC 2821 CACGCTGGGC TACTTGATCT GCGGCTTTCG GGTCGTGCTC ATGTACCGCT TCGAGGAGGA 2881 GCTATTCTTG CGCAGCTTGC AAGACTATAA GATTCAATCT GCCCTGCTGG TGCCCACACT 2941 ATTTAGCTTC TTCGCTAAGA GCACTCTCAT CGACAAGTAC GACCTAAGCA ACTTGCACGA 3001 GATCGCCAGC GGCGGGGCGC CGCTCAGCAA GGAGGTAGGT GAGGCCGTGG CCAAACGCTT 3061 CCACCTACCA GGCATCCGCC AGGGCTACGG CCTGACAGAA ACAACCAGCG CCATTCTGAT 3121 CACCCCCGAA GGGGACGACA AGCCTGGCGC AGTAGGCAAG GTGGTGCCCT TCTTCGAGGC 3181 TAAGGTGGTG GACTTGGACA CCGGTAAGAC ACTGGGTGTG AACCAGCGCG GCGAGCTGTG 3241 CGTCCGTGGC CCCATGATCA TGAGCGGCTA CGTTAACAAC CCCGAGGCTA CAAACGCTCT 3301 CATCGACAAG GACGGCTGGC TGCACAGCGG CGACATCGCC TACTGGGACG AGGACGAGCA 3361 CTTCTTCATC GTGGACCGGC TGAAGAGCCT GATCAAATAC AAGGGCTACC AGGTAGCCCC 3421 AGCCGAACTG GAGAGCATCC TGCTGCAACA CCCCAACATC TTCGACGCCG GGGTCGCCGG 3481 CCTGCCCGAC GACGATGCCG GCGAGCTGCC CGCCGCAGTC GTCGTGCTGG AACACGGTAA 3541 AACCATGACC GAGAAGGAGA TCGTGGACTA TGTGGCCAGC CAGGTTACAA CCGCCAAGAA 3601 GCTGCGCGGT GGTGTTGTGT TCGTGGACGA GGTGCCTAAA GGACTGACCG GCAAGTTGGA 3661 CGCCCGCAAG ATCCGCGAGA TTCTCATTAA GGCCAAGAAG GGCGGCAAGA TCGCCGTGAA 3721 TTCTCACGGC TTCCCTCCCG AGGTGGAGGA GCAGGCCGCC GGCACCCTGC CCATGAGCTG 3781 CGCCCAGGAG AGCGGCATGG ATAGACACCC TGCTGCTTGC GCCAGCGCCA GGATCAACGT 3841 CTAAGGCCGC GACTCTAGAG TCGGGGCGGC CGGCCGCTTC GAGCAGACAT GATAAGATAC 3901 ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA 3961 ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC 4021 AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC 4081 AAGTAAAACC TCTACAAATG TGGTAAAATC GATAAGGATC CGTCGACCGA TGCCCTTGAG 4141 AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT 4201 TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT 4261 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 4321 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 4381 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 4441 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 4501 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 4561 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 4621 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 4681 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 4741 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 4801 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 4861 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 4921 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 4981 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 5041 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 5101 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 5161 AAAGTATATA TGAGTAAACT TGGTCTGACA GCGGCCGCAA ATGCTAAACC ACTGCAGTGG 5221 TTACCAGTGC TTGATCAGTG AGGCACCGAT CTCAGCGATC TGCCTATTTC GTTCGTCCAT 5281 AGTGGCCTGA CTCCCCGTCG TGTAGATCAC TACGATTCGT GAGGGCTTAC CATCAGGCCC 5341 CAGCGCAGCA ATGATGCCGC GAGAGCCGCG TTCACCGGCC CCCGATTTGT CAGCAATGAA 5401 CCAGCCAGCA GGGAGGGCCG AGCGAAGAAG TGGTCCTGCT ACTTTGTCCG CCTCCATCCA 5461 GTCTATGAGC TGCTGTCGTG ATGCTAGAGT AAGAAGTTCG CCAGTGAGTA GTTTCCGAAG 5521 AGTTGTGGCC ATTGCTACTG GCATCGTGGT ATCACGCTCG TCGTTCGGTA TGGCTTCGTT 5581 CAACTCTGGT TCCCAGCGGT CAAGCCGGGT CACATGATCA CCCATATTAT GAAGAAATGC 5641 AGTCAGCTCC TTAGGGCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCGG TGTTGTCGCT 5701 CATGGTAATG GCAGCACTAC ACAATTCTCT TACCGTCATG CCATCCGTAA GATGCTTTTC 5761 CGTGACCGGC GAGTACTCAA CCAAGTCGTT TTGTGAGTAG TGTATACGGC GACCAAGCTG 5821 CTCTTGCCCG GCGTCTATAC GGGACAACAC CGCGCCACAT AGCAGTACTT TGAAAGTGCT 5881 CATCATCGGG AATCGTTCTT CGGGGCGGAA AGACTCAAGG ATCTTGCCGC TATTGAGATC 5941 CAGTTCGATA TAGCCCACTC TTGCACCCAG TTGATCTTCA GCATCTTTTA CTTTCACCAG 6001 CGTTTCGGGG TGTGCAAAAA CAGGCAAGCA AAATGCCGCA AAGAAGGGAA TGAGTGCGAC 6061 ACGAAAATGT TGGATGCTCA TACTCGTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 6121 TTACTAGTAC GTCTCTCAAG GATAAGTAAG TAATATTAAG GTACGGGAGG TATTGGACAG 6181 GCCGCAATAA AATATCTTTA TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGAATCGA 6241 TAGTACTAAC ATACGCTCTC CATCAAAACA AAACGAAACA AAACAAACTA GCAAAATAGG 6301 CTGTCCCCAG TGCAAGTGCA GGTGCCAGAA CATTTCTCT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???