Catalog No. PVT11295
Packing 2ug
LOCUS Exported 5166 bp ds-DNA circular SYN 22-OCT-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5166)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..5166
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 7..286
/label=P43 promoter
misc_feature 290..299
/label=RBS
misc_feature 307..338
/label=MCS
CDS complement(396..1772)
/codon_start=1
/gene="Bacillus subtilis tetL"
/product="tetracycline efflux protein"
/label=TcR
/note="confers resistance to tetracycline"
/translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
"
CDS complement(1979..2983)
/codon_start=1
/gene="repB"
/product="RepB replication protein"
/label=repB
/note="from Enterococcus faecalis plasmid pAM-alpha-1"
/db_xref="GI:22652809"
/protein_id="AAN03827.1"
/translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
promoter 3480..3584
/gene="bla"
/label=AmpR promoter
CDS 3585..4445
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4610..5154
/direction=RIGHT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
ORIGIN
1 aagctttgat aggtggtatg ttttcgcttg aacttttaaa tacagccatt gaacatacgg
61 ttgatttaat aactgacaaa catcaccctc ttgctaaagc ggccaaggac gctgccgccg
121 gggctgtttg cgtttttgcc gtgatttcgt gtatcattgg tttacttatt tttttgccaa
181 agctgtaatg gctgaaaatt cttacattta ttttacattt ttagaaatgg gcgtgaaaaa
241 aagcgcgcga ttatgtaaaa tataaagtga tagcggtacc attataggta agagaggaac
301 gtacacagat ctgcaggtcg acggatcccc gggaattcct gttataaaaa aaggatcaat
361 tttgaactct ctcccaaagt tgatccctta acgatttaga aatccctttg agaatgttta
421 tatacattca aggtaaccag ccaactaatg acaatgattc ctgaaaaaag taataacaaa
481 ttactataca gataagttga ctgatcaact tccataggta acaacctttg atcaagtaag
541 ggtatggata ataaaccacc tacaattgca atacctgttc cctctgataa aaagctggta
601 aagttaagca aactcattcc agcaccagct tcctgctgtt tcaagctact tgaaacaatt
661 gttgatataa ctgttttggt gaacgaaagc ccacctaaaa caaatacgat tataattgtc
721 atgaaccatg atgttgtttc taaaagaaag gaagcagtta aaaagctaac agaaagaaat
781 gtaactccga tgtttaacac gtataaagga cctcttctat caacaagtat cccaccaatg
841 tagccgaaaa taatgacact cattgttcca gggaaaataa ttacacttcc gatttcggca
901 gtacttagct ggtgaacatc tttcatcata taaggaacca tagagacaaa ccctgctact
961 gttccaaata taattccccc acaaagaact ccaatcataa aaggtatatt tttccctaat
1021 ccgggatcaa caaaaggatc tgttactttc ctgatatgtt ttacaaatat caggaatgac
1081 agcacgctaa cgataagaaa agaaatgcta tatgatgttg taaacaacat aaaaaataca
1141 atgcctacag acattagtat aattcctttg atatcaaaat gaccttttat ccttacttct
1201 ttctttaata atttcataag aaacggaaca gtgataattg ttatcatagg aatgagtaga
1261 agataggacc aatgaatata atgggctatc attccaccaa tcgctggacc gactccttct
1321 cccatggcta ctatcgatcc aataagacca aatgctttac ccctattttc ctttggaata
1381 tagcgcgcaa ctacaaccat tacgagtgct ggaaatgcag ctgcaccagc cccttgaata
1441 aaacgagcca taataagtaa ggaaaagaaa gaatggccaa caaacccaat taccgacccg
1501 aaacaattta ttataattcc aaataggagt aaccttttga tgcctaattg atcagatagc
1561 tttccatata cagctgttcc aatggaaaag gttaacataa aggctgtgtt cacccagttt
1621 gtactcgcag gtggtttatt aaaatcattt gcaatatcag gtaatgagac gttcaaaacc
1681 atttcattta atacgctaaa aaaagataaa atgcaaagcc aaattaaaat ttggttgtgt
1741 cgtaaattcg attgtgaata ggatgtattc acatttcacc ctccaataat gagggcagac
1801 gtagtttata gggttaatga tacgcttccc tcttttaatt gaaccctgtt acattcatta
1861 ttcattacac ttcataatta attcctccta aacttgatta aaacatttta ccacatataa
1921 actaagtttt aaattcagta tttcatcact tatacaacaa tatggcccgt ttgttgaact
1981 actctttaat aaaataattt ttccgttccc aattccacat tgcaataata gaaaatccat
2041 cttcatcggc tttttcgtca tcatctgtat gaatcaaatc gccttcttct gtgtcatcaa
2101 ggtttaattt tttatgtatt tcttttaaca aaccaccata ggagattaac cttttacggt
2161 gtaaaccttc ctccaaatca gacaaacgtt tcaaattctt ttcttcatca tcggtcataa
2221 aatccgtatc ctttacagga tattttgcag tttcgtcaat tgccgattgt atatccgatt
2281 tatatttatt tttcggtcga atcatttgaa cttttacatt tggatcatag tctaatttca
2341 ttgccttttt ccaaaattga atccattgtt tttgattcac gtagttttct gtattcttaa
2401 aataagttgg ttccacacat accaatacat gcatgtgctg attataagaa ttatctttat
2461 tatttattgt cacttccgtt gcacgcataa aaccaacaag atttttatta atttttttat
2521 attgcatcat tcggcgaaat ccttgagcca tatctgacaa actcttattt aattcttcgc
2581 catcataaac atttttaact gttaatgtga gaaacaacca acgaactgtt ggcttttgtt
2641 taataacttc agcaacaacc ttttgtgact gaatgccatg tttcattgct ctcctccagt
2701 tgcacattgg acaaagcctg gatttacaaa accacactcg atacaacttt ctttcgcctg
2761 tttcacgatt ttgtttatac tctaatattt cagcacaatc ttttactctt tcagcctttt
2821 taaattcaag aatatgcaga agttcaaagt aatcaacatt agcgattttc ttttctctcc
2881 atggtctcac ttttccactt tttgtcttgt ccactaaaac ccttgatttt tcatctgaat
2941 aaatgctact attaggacac ataatattaa aagaaacccc catctattta gttatttgtt
3001 tggtcactta taactttaac agatggggtt tttctgtgca accaatttta agggttttcc
3061 aatactttaa aacacataca taccaacact tcaacgcacc tttcagcaac taaaataaaa
3121 atgacgttat ttctatatgt atcaagataa gaaagaacaa gttcaaaacc atcaaaaaaa
3181 gacacctttt caggtgcttt ttttatttta taaactcatt ccctgatctc gacttcgttc
3241 tttttttacc tctcggttat gagttagttc aaattcgttc tttttaggtt ctaaatcgtg
3301 tttttcttgg aattgtgctg ttttatcctt taccttgtct acaaacccct taaaaacgtt
3361 tttaaaggct tttaagcgtc tgtacgttcc ttaaggaatt attccttagt gctttctagg
3421 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtcc
3481 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
3541 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
3601 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
3661 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
3721 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
3781 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
3841 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
3901 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
3961 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
4021 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
4081 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
4141 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
4201 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
4261 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
4321 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
4381 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
4441 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
4501 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
4561 gtgagttttc gttccactga gcgtcagacc ccttaataag atgatcttct tgagatcgtt
4621 ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggaggttttt
4681 cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgca ggagcgcagt
4741 caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct
4801 ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac
4861 tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata
4921 cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa
4981 acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac
5041 gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca
5101 ctgatttgag cgtcagattt cgtgatgctt gtcagggggc ggagcctatg gaaaaacgct
5161 ttgccc
//
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.
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