pJYS2_crtYf
Catalog No. PVT10657
Packing 2ug
Function E.coli Editing plasmids
Resistance Spe
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon repA
pJYS2_crtYf Descrption
pJYS2_crtYf is an E. coli gRNA expression plasmid.
pJYS2_crtYf Sequence
LOCUS Exported 4380 bp ds-DNA circular SYN 21-DEC-2017
DEFINITION Constitutive transcription of crRNA of crtYf, Spectinomycin
resistance.
FEATURES Location/Qualifiers
source 1..4380
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 58..143
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
misc_feature 148..188
/label=crRNA
CDS complement(522..1985)
/codon_start=1
/label=repA
/translation="MTLADPQDTVTASAWKFSADLFDTHPELALRSRGWTAEDRRELLA
HLGRESFQGSKTRDFASAWIKNPDTGETQPKLYRAGSKALTRCQYVALTHAQHAAVIVL
DIDVPSHQAGGKIEHVNPQVYAILEKWARLEKAPAWIGVNPLSGKCQLIWLIDPVYAAA
GKTSPNMRLLAATTEEMTRVFGADQAFSHRLSRWPLHVSDDPTAYKWHCQHDRVDRLAD
LMEIARTMTGSQKPKKYIEQDFSSGRARIEAAQRATAEAKALAILDASLPSALDASGDL
IDGVRVLWTNPERAARDETAFRHALTVGYQLKAAGERLKDAKIIDAYEVAYNVAQAVGA
DGREPDLPAMRDRLTMARRVRGYVAKGQPVVPARRVETQSSRGRKALATMGRRGAATSN
ARRWADPESKYAQETRQRLAEANKRREMTGELLELRVKTAILDARSQSVADPSTRELAG
ELGVSERRIQQVRKALGMEAKRGRPRAEN"
CDS complement(2391..3065)
/codon_start=1
/label=Spe
/translation="MFGSGVESGLKPNSDLDFLVVVSEPLTDQSKEILIQKIRPISKKI
GDKSNLRYIELTIIIQQEMVPWNHPPKQEFIYGEWLQELYEQGYIPQKELNSDLTIMLY
QAKRKNKRIYGNYDLEELLPDIPFSDVRRAIMDSSEELIDNYQDDETNSILTLCRMILT
MDTGKIIPKDIAGNAVAESSPLEHRERILLAVRSYLGENIEWTNENVNLTINYLNNRLK
KL"
rep_origin 3466..4054
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator 4319..4346
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
ORIGIN
1 tcctgcccgc caccctccgg gccgttgctt cgcaacgttc aaatccgctc ccggcggatt
61 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt
121 cgactgagcc tttcgtttta tttaaatttc ctgccctatg gttgcctgat ctacaacagt
181 agaaattcgg atccattata cctaggactg agctagctgt caatctagac tcgaggagat
241 ttttgagggg gagggagtcg aggaagagcc agagcagaag gcgggggaac cgttctctgc
301 cgacagcgtg agccccctta aaaatcaggc cggggaggaa ccggggaggg atcagagcta
361 ggagcgagac accctaaagg gggggaaccg ttttctgctg acggtgtttc gtttattagt
421 tttcagcccg tggatagcgg agggtgaggg caagtgagag ccagagcaag gacgggaccc
481 ctaaaggggg gaaccgtttt ctgctgacgg tgtttcgttt attagttttc agcccgtgga
541 cggccgcgtt tagcttccat tccaagtgcc tttctgactt gttggatgcg cctttcactg
601 acacctagtt cgcctgcaag ctcacgagtc gagggatcag caaccgattg agaacgggca
661 tccaggatcg cagttttgac gcgaagttcg agcaactcgc ctgtcatttc tcggcgtttg
721 tttgcttccg ctaatcgctg tcgcgtctcc tgcgcatact tactttctgg gtcagcccat
781 ctgcgtgcat tcgatgtagc tgcgccccgt cgccccatcg tcgctagagc tttccgccct
841 cggctgctct gcgtttccac ccgacgagca gggacgactg gctggccttt agccacgtag
901 ccgcgcacac gacgcgccat cgtcaggcga tcacgcatgg cgggaagatc cggctcccgg
961 ccgtctgcac cgaccgcctg ggcaacgttg tacgccactt catacgcgtc gatgatcttg
1021 gcatctttta ggcgctcacc agcagctttg agctggtatc ccacggtcaa cgcgtggcga
1081 aacgcggtct cgtcgcgcgc tgcgcgctct ggatttgtcc agagcactcg cacgccgtcg
1141 atcaggtcgc cggacgcgtc cagggcgctc ggcaggctcg cgtccaaaat cgctagcgcc
1201 ttggcttctg cggtggcgcg ttgtgccgct tcaatgcggg cgcgtccgct ggaaaagtcc
1261 tgctcaatgt actttttcgg cttctgtgat ccggtcatcg ttcgagcaat ctccattagg
1321 tcggccagcc gatccacacg atcatgctgg cagtgccatt tataggctgt cggatcgtct
1381 gagacgtgca gcggccaccg gctcagccta tgcgaaaaag cctggtcagc gccgaaaaca
1441 cgagtcattt cttccgtcgt tgcagccagc aggcgcatat ttgggctggt tttacctgct
1501 gcggcataca ccgggtcaat gagccagatg agctggcatt tcccgctcag cggattcacg
1561 ccgatccaag ccggcgcttt ttctaggcgt gcccatttct ctaaaatcgc gtagacctgc
1621 gggtttacgt gctcaatctt cccgccggcc tggtggctgg gcacatcgat gtcaagcacg
1681 atcaccgcgg catgttgcgc gtgcgtcagc gcaacgtact ggcaccgcgt cagcgctttt
1741 gagccagccc ggtagagctt tggttgggtt tcgccggtat ccgggttttt aatccaggcg
1801 ctcgcgaaat ctcttgtctt gctgccctgg aagctttcgc gtcccaggtg agcgagcagt
1861 tcgcggcgat cttctgccgt ccagccgcgt gagcgcagcg ctagttcggg gtgggtgtcg
1921 aacagatcgg cggaaaattt ccacgcgcta gctgtgactg tgtcctgcgg atcggctaga
1981 gtcatgtctt gagtgctttc tcccagctga tgactggggg ttagccgacg ccctgtgagt
2041 tcccgctcac ggggcgttca actttttcag gtatttgtgc agcttatcgt gttttcttcg
2101 taaatgaacg cttaactacc ttgttaaacg tggcaaatag gcaggattga tggctttcat
2161 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
2221 tttcgaaggg caccaataac tgccttaaaa aaagatctgt acatcgatat catatgcaag
2281 ggtttattgt tttctaaaat ctgattacca attagaatga atatttccca aatattaaat
2341 aataaaacaa aaaaattgaa aaaagtgttt ccaccatttt ttcaattttt ttataatttt
2401 tttaatctgt tatttaaata gtttatagtt aaatttacat tttcattagt ccattcaata
2461 ttctctccaa gataactacg aactgctaac aaaattctct ccctatgttc taatggagaa
2521 gattcagcca ctgcatttcc cgcaatatct tttggtatga ttttacccgt gtccatagtt
2581 aaaatcatac ggcataaagt taatatagag ttggtttcat catcctgata attatctatt
2641 aattcctctg acgaatccat aatggctctt ctcacatcag aaaatggaat atcaggtagt
2701 aattcctcta agtcataatt tccgtatatt cttttatttt ttcgttttgc ttggtaaagc
2761 attatggtta aatctgaatt taattccttc tgaggaatgt atccttgttc ataaagctct
2821 tgtaaccatt ctccataaat aaattcttgt ttgggaggat gattccacgg taccatttct
2881 tgctgaataa taattgttaa ttcaatatat cgtaagttgc ttttatctcc tatttttttt
2941 gaaataggtc taattttttg tataagtatt tctttacttt gatctgtcaa tggttcagat
3001 acgacgacta aaaagtcaag atcactattt ggttttagtc cactctcaac tcctgatcca
3061 aacatgtaag taccaataag gttatttttt aaatgtttcc gaagtatttt tttcacttta
3121 ttaatttgtt cgtatgtatt caaatatatc ctcctcacta ttttgattag tacctatttt
3181 atatccatag ttgttaatta aataaactta atttagttta tttatagatt tcattggctt
3241 ctaaattttt tatctagata ataattattt tagttaattt tattctagat tatatatgat
3301 atgatctttc atttccataa aactaaagta agtgtaaacc tattcattgt tttaaaaata
3361 tctcttgcca gtcacgttac gttattagtt catgaccaaa atcccttaac gtgagttttc
3421 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
3481 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
3541 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
3601 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
3661 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
3721 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
3781 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
3841 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
3901 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
3961 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
4021 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
4081 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
4141 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
4201 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
4261 tacgcatctg tgcggtattt cacaccgcat atgaagctta agagtttgta gaaacgcaaa
4321 aaggccatcc gtcaggatgg ccttctgctt aatttgatgc ctggcagttt atggcgggcg
//
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