pKyat3-Fluc plasmid

PVT111775

pKyat3-Fluc plasmid Information

Plasmid name: pKyat3(mouse)-Fluc
Alias:
Gene lenght: 2000
Host: Mammalian cells
Use(s): Signal report
Fragment type: Promoter
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: Kyat3
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: RVP3
Reverse primer:
Copy number: High
Induction:

pKyat3-Fluc plasmid Description

pKyat3-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pKyat3-Fluc plasmid Sequence

1 ggtaccATGT GCAGTGAACC CACATCTAGA AGACTACTGT TGAACTTTTC CCAAGGGCAG 61 TTTTGATGAT TATCATCTAC ATATACACCT CATATCTTCA ATCACTTTCC CTACTATAAT 121 AATATGTAAA TTTAAGACAA CAGTTCGAAG ATGATTTGAC TAGTTTAAAT TGCAAGTGCT 181 AGCAGCTGAA CTGCATTCAA CCCAGAGAAT ATATGTGGAT GGAGACAGAA AGAGACAGAG 241 AAACCTTTGC AATCTTTTTG AGGGTAAATG CACACTTTCT GCTAAGGCTT CTGAAACATT 301 TAAATTAATG CCAACATCAC TATTATTGCC TAGCTGGGAA GCACTAATTT ACAACTCACA 361 AGCTGAGCAA GCATCTGCAT GTCTGTATCT CTTGGCATTC TAGAGGCTGA AGCAGAAGGG 421 TGTCTGAATT TGGGGGACAA TGGGCTAAGT AGCTAAAACA GTCAAGGGTG CACAGTGAGA 481 TCCTATCTTG AAAACAAAAG GACAAACAAA GAACAGTAAA CTCAACTTGT AGATTCCATG 541 TTTTGCTCCA AGATGGCTGC AGCCAGCAAA TTGACATTCT AATGAGCAAG GAAACCACAA 601 GTCAAAGAAA CGGTAAAGGC AGCAGTCCAC CATGTGGGCA GTGTAATTAT ATCAGAAACT 661 AGGAACACAG TGGTCATGAA ACATACCCTG TGTTTTGAAA TAAGAAACTA GCAGCTTACT 721 GTAATGGTTG TATGTGAAAA GGAGAAGAGA GAGAAAGGAA AGGGACACCG AGCAGCCACT 781 TAGCCTCAAA AACCTTGAGA ACGAACTGCG ATGTTCCCCA ATTCAACCTC AGCAATATCA 841 ACAGAACACC GACTGCAGAT AAAGCCATTC CAGATCGTCA TGAGTTTTAG GTCCAAAGGG 901 GAAAACTCCA AGAATCCCGA ATATGGACAG ACCCGCTGAC TGGGTTCAAA ATGAAGGCTT 961 GCCTCACCTC AAGCTCCACT GTAGAATTTC AGGGAGAGAC AAAAGTTCAC CCTCATCCTT 1021 AAGGACCTCT GAAACCTTCT GTCTGCCAGG AAAATCCACT TGCCTATTAA GATACAAAGT 1081 GCCTACCAAA GCCACAGAGC TCTCAAGCCC TCCTCCCCTT CTATTCTAAA CTTCCAGGCT 1141 GTTCCAATTC ACAGGACAAA CACACACACA CACACACACA CACACACACA CACACACACA 1201 CACACCTGAA GCTGCAGCCA CCGGTTTTTC TTTATAACTC GCAAGGTTTT TCCTGCTATT 1261 CCCTCTGCTC TGGAGAGACA GCCCTTGCCA GTCTGCCTCT CTGCTCTCTT CCCATAACTG 1321 CTTTGGACTT TCCCCAAATC CTCTAGTTAT TCTCTCTCTC CTATATCTTA GGTAAACATC 1381 TTCTTCCCAC CATGGGTCGG TCAGTTCAGC AGCCTGCCTG TGCCCTTGCT TACAATGAGC 1441 ACCTTTAATG TTCGTAACAA AATACAGAAG CGCTTGGCTC ACTGTGGCAA GGTCAGTGCA 1501 TCCAACCAAA TCCGCTTTTG TTCCTTCCTA CAGGCGTGAG TACAGCGTTC AGCCGCTCTC 1561 GTGAACTACA ACACCTCGAC TCCTTTAAAC GCAAGAGGAA GAAAATCTAA CCCCCAAGCT 1621 GTGCTCTTAA CAGCTTGTCC TCCCGCTTCA GTGTCACTTC CTCCGCCTTT TAGGTCCTGC 1681 ATCACTTTAC AGTTCCGAAG GAAAGATTCC CTCTCCCTCC TCTCTCCCCA TCAGAGGGAA 1741 CACATAGACA ACTACACAGG CCGGCCTCTC AAGCATTTTC AAGTCTTTCT CCTAGAACCT 1801 GCTTCGAATC CACGCAGCGG AGATTTAAAA ACAGTTCTGC TCCAAACAGT CGCACACTTC 1861 CACTTTTTCC TGGATACTCA GCCAAGGCCA CGCCCTGTCC CCTAGCCTGG GAGCTTCTTG 1921 TGCACTCTCA CAGGGCGCGC CACGATTGGA GAAATCCGTA ACCAATCTGT AGCCTTAGCC 1981 CTGATACCGG AGCCAATCCC TGGTCAaagc ttggcattcc ggtactgttg gtaaagccac 2041 catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg 2101 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat 2161 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc 2221 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt 2281 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt 2341 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat 2401 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca 2461 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg 2521 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga 2581 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg 2641 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc 2701 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt 2761 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt 2821 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta 2881 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct 2941 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc 3001 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata 3061 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg 3121 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa 3181 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg 3241 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc 3301 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc 3361 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca 3421 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact 3481 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga 3541 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga 3601 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat 3661 aaaggccaag aagggcggaa agatcgccgt gtaattctag agtcggggcg gccggccgct 3721 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 3781 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 3841 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 3901 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga 3961 tccgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg 4021 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg 4081 tgccggcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 4141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 4201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 4261 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 4321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 4381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 4441 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg 4501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 4561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 4621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 4681 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 4741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 4801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 4861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 4921 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 4981 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 5041 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 5101 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 5161 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 5221 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 5281 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 5341 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 5401 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 5461 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 5521 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 5581 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 5641 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 5701 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 5761 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 5821 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 5881 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 5941 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 6001 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt 6061 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 6121 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 6181 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 6241 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 6301 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 6361 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 6421 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt 6481 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 6541 ctattacgcc agcccaagct accatgataa gtaagtaata ttaaggtacg ggaggtactt 6601 ggagcggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg 6661 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa 6721 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctatcga ta

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.