pLenti-3×NAFT-miniP-Fluc-CMV-FCGR3A-Puro plasmid

PVT109004

pLenti-3×NAFT-miniP-Fluc-CMV-FCGR3A-Puro plasmid Information

Plasmid name: pLenti-3×NAFT-miniP-Fluc-CMV-FCGR3A(human)-Puro
Alias: NM_000569.8;CD16; CD16-II; CD16A; FCG3; FCGR3; FCGRIII; FcGRIIIA; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20
Gene lenght: 765
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CPPT-F
Reverse primer:
Copy number: High
Induction:

pLenti-3×NAFT-miniP-Fluc-CMV-FCGR3A-Puro plasmid Description

pLenti-3×NAFT-miniP-Fluc-CMV-FCGR3A-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLenti-3×NAFT-miniP-Fluc-CMV-FCGR3A-Puro plasmid Sequence

1 CTTATGCAAT ACTCTTGTAG TCTTGCAACA TGGTAACGAT GAGTTAGCAA CATGCCTTAC 61 AAGGAGAGAA AAAGCACCGT GCATGCCGAT TGGTGGAAGT AAGGTGGTAC GATCGTGCCT 121 TATTAGGAAG GCAACAGACG GGTCTGACAT GGATTGGACG AACCACTGAA TTGCCGCATT 181 GCAGAGATAT TGTATTTAAG TGCCTAGCTC GATACATAAA CGGGTCTCTC TGGTTAGACC 241 AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA 301 GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA 361 GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA 421 CTTGAAAGCG AAAGGGAAAC CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC 481 GCGCACGGCA AGAGGCGAGG GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG 541 AGGCTAGAAG GAGAGAGATG GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC 601 GCGATGGGAA AAAATTCGGT TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT 661 AGTATGGGCA AGCAGGGAGC TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC 721 AGAAGGCTGT AGACAAATAC TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA 781 ACTTAGATCA TTATATAATA CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT 841 AAAAGACACC AAGGAAGCTT TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC 901 CGCACAGCAA GCGGCCGCTG ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA 961 GAAGTGAATT ATATAAATAT AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA 1021 AGGCAAAGAG AAGAGTGGTG CAGAGAGAAA AAAGAGCAGT GGGAATAGGA GCTTTGTTCC 1081 TTGGGTTCTT GGGAGCAGCA GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGTAC 1141 AGGCCAGACA ATTATTGTCT GGTATAGTGC AGCAGCAGAA CAATTTGCTG AGGGCTATTG 1201 AGGCGCAACA GCATCTGTTG CAACTCACAG TCTGGGGCAT CAAGCAGCTC CAGGCAAGAA 1261 TCCTGGCTGT GGAAAGATAC CTAAAGGATC AACAGCTCCT GGGGATTTGG GGTTGCTCTG 1321 GAAAACTCAT TTGCACCACT GCTGTGCCTT GGAATGCTAG TTGGAGTAAT AAATCTCTGG 1381 AACAGATTTG GAATCACACG ACCTGGATGG AGTGGGACAG AGAAATTAAC AATTACACAA 1441 GCTTAATACA CTCCTTAATT GAAGAATCGC AAAACCAGCA AGAAAAGAAT GAACAAGAAT 1501 TATTGGAATT AGATAAATGG GCAAGTTTGT GGAATTGGTT TAACATAACA AATTGGCTGT 1561 GGTATATAAA ATTATTCATA ATGATAGTAG GAGGCTTGGT AGGTTTAAGA ATAGTTTTTG 1621 CTGTACTTTC TATAGTGAAT AGAGTTAGGC AGGGATATTC ACCATTATCG TTTCAGACCC 1681 ACCTCCCAAC CCCGAGGGGA CCCAGACCAC AATTTTAAAA GAAAAGGGGG GATTGGGGGG 1741 TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC TAAAGAATTA 1801 CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG CAGAGATCCA 1861 CTTTGGCGCC GGCTCGAGaa ttatctagag tcgcgggatc cTACCTGAGC TCGCTAGCgG 1921 AGGAAAAACT GTTTCATACA GAAGGCGTGG AGGAAAAACT GTTTCATACA GAAGGCGTGG 1981 AGGAAAAACT GTTTCATACA GAAGGCGTAG ATCTAGACTC TAGAGGGTAT ATAATGGAAG 2041 CTCGAATTCC AGCTTGGCAT TCCGGTACTG TTGGTAAAAA GCTTGGCAAT CCGGTACTGT 2101 TGGTAAAGCC ACCATGGAAG ATGCCAAAAA CATTAAGAAG GGCCCAGCGC CATTCTACCC 2161 ACTCGAAGAC GGGACCGCCG GCGAGCAGCT GCACAAAGCC ATGAAGCGCT ACGCCCTGGT 2221 GCCCGGCACC ATCGCCTTTA CCGACGCACA TATCGAGGTG GACATTACCT ACGCCGAGTA 2281 CTTCGAGATG AGCGTTCGGC TGGCAGAAGC TATGAAGCGC TATGGGCTGA ATACAAACCA 2341 TCGGATCGTG GTGTGCAGCG AGAATAGCTT GCAGTTCTTC ATGCCCGTGT TGGGTGCCCT 2401 GTTCATCGGT GTGGCTGTGG CCCCAGCTAA CGACATCTAC AACGAGCGCG AGCTGCTGAA 2461 CAGCATGGGC ATCAGCCAGC CCACCGTCGT ATTCGTGAGC AAGAAAGGGC TGCAAAAGAT 2521 CCTCAACGTG CAAAAGAAGC TACCGATCAT ACAAAAGATC ATCATCATGG ATAGCAAGAC 2581 CGACTACCAG GGCTTCCAAA GCATGTACAC CTTCGTGACT TCCCATTTGC CACCCGGCTT 2641 CAACGAGTAC GACTTCGTGC CCGAGAGCTT CGACCGGGAC AAAACCATCG CCCTGATCAT 2701 GAACAGTAGT GGCAGTACCG GATTGCCCAA GGGCGTAGCC CTACCGCACC GCACCGCTTG 2761 TGTCCGATTC AGTCATGCCC GCGACCCCAT CTTCGGCAAC CAGATCATCC CCGACACCGC 2821 TATCCTCAGC GTGGTGCCAT TTCACCACGG CTTCGGCATG TTCACCACGC TGGGCTACTT 2881 GATCTGCGGC TTTCGGGTCG TGCTCATGTA CCGCTTCGAG GAGGAGCTAT TCTTGCGCAG 2941 CTTGCAAGAC TATAAGATTC AATCTGCCCT GCTGGTGCCC ACACTATTTA GCTTCTTCGC 3001 TAAGAGCACT CTCATCGACA AGTACGACCT AAGCAACTTG CACGAGATCG CCAGCGGCGG 3061 GGCGCCGCTC AGCAAGGAGG TAGGTGAGGC CGTGGCCAAA CGCTTCCACC TACCAGGCAT 3121 CCGCCAGGGC TACGGCCTGA CAGAAACAAC CAGCGCCATT CTGATCACCC CCGAAGGGGA 3181 CGACAAGCCT GGCGCAGTAG GCAAGGTGGT GCCCTTCTTC GAGGCTAAGG TGGTGGACTT 3241 GGACACCGGT AAGACACTGG GTGTGAACCA GCGCGGCGAG CTGTGCGTCC GTGGCCCCAT 3301 GATCATGAGC GGCTACGTTA ACAACCCCGA GGCTACAAAC GCTCTCATCG ACAAGGACGG 3361 CTGGCTGCAC AGCGGCGACA TCGCCTACTG GGACGAGGAC GAGCACTTCT TCATCGTGGA 3421 CCGGCTGAAG AGCCTGATCA AATACAAGGG CTACCAGGTA GCCCCAGCCG AACTGGAGAG 3481 CATCCTGCTG CAACACCCCA ACATCTTCGA CGCCGGGGTC GCCGGCCTGC CCGACGACGA 3541 TGCCGGCGAG CTGCCCGCCG CAGTCGTCGT GCTGGAACAC GGTAAAACCA TGACCGAGAA 3601 GGAGATCGTG GACTATGTGG CCAGCCAGGT TACAACCGCC AAGAAGCTGC GCGGTGGTGT 3661 TGTGTTCGTG GACGAGGTGC CTAAAGGACT GACCGGCAAG TTGGACGCCC GCAAGATCCG 3721 CGAGATTCTC ATTAAGGCCA AGAAGGGCGG CAAGATCGCC GTGTAAGGTG GCagttccgc 3781 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 3841 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 3901 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 3961 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 4021 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 4081 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 4141 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 4201 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 4261 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc 4321 catccacgct gttttgacct ccatagaaga caccgactct actagaggat cgctagcgct 4381 accggactca gatctcgagc caccatgtgg cagctgctcc tcccaactgc tctgctactt 4441 ctagtttcag ctggcatgcg gactgaagat ctcccaaagg ctgtggtgtt cctggagcct 4501 caatggtaca gggtgctcga gaaggacagt gtgactctga agtgccaggg agcctactcc 4561 cctgaggaca attccacaca gtggtttcac aatgagagcc tcatctcaag ccaggcctcg 4621 agctacttca ttgacgctgc cacagtcgac gacagtggag agtacaggtg ccagacaaac 4681 ctctccaccc tcagtgaccc ggtgcagcta gaagtccata tcggctggct gttgctccag 4741 gcccctcggt gggtgttcaa ggaggaagac cctattcacc tgaggtgtca cagctggaag 4801 aacactgctc tgcataaggt cacatattta cagaatggca aaggcaggaa gtattttcat 4861 cataattctg acttctacat tccaaaagcc acactcaaag acagcggctc ctacttctgc 4921 agggggcttt ttgggagtaa aaatgtgtct tcagagactg tgaacatcac catcactcaa 4981 ggtttggcag tgtcaaccat ctcatcattc tttccacctg ggtaccaagt ctctttctgc 5041 ttggtgatgg tactcctttt tgcagtggac acaggactat atttctctgt gaagacaaac 5101 attcgaagct caacaagaga ctggaaggac cataaattta aatggagaaa ggaccctcaa 5161 gacaaatgaG CTAGCCTACC GGGTAGGGGA GGCGCTTTTC CCAAGGCAGT CTGGAGCATG 5221 CGCTTTAGCA GCCCCGCTGG GCACTTGGCG CTACACAAGT GGCCTCTGGC CTCGCACACA 5281 TTCCACATCC ACCGGTAGGC GCCAACCGGC TCCGTTCTTT GGTGGCCCCT TCGCGCCACC 5341 TTCTACTCCT CCCCTAGTCA GGAAGTTCCC CCCCGCCCCG CAGCTCGCGT CGTGCAGGAC 5401 GTGACAAATG GAAGTAGCAC GTCTCACTAG TCTCGTGCAG ATGGACAGCA CCGCTGAGCA 5461 ATGGAAGCGG GTAGGCCTTT GGGGCAGCGG CCAATAGCAG CTTTGCTCCT TCGCTTTCTG 5521 GGCTCAGAGG CTGGGAAGGG GTGGGTCCGG GGGCGGGCTC AGGGGCGGGC TCAGGGGCGG 5581 GGCGGGCGCC CGAAGGTCCT CCGGAGGCCC GGCATTCTGC ACGCTTCAAA AGCGCACGTC 5641 TGCCGCGCTG TTCTCCTCTT CCTCATCTCC GGGCCTTTCG ACCTGCAGCC CAAGCTTACC 5701 ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC CAGGGCCGTA 5761 CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT CGATCCGGAC 5821 CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC 5881 ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG 5941 AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT 6001 TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG 6061 CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC 6121 AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG 6181 GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC 6241 GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA 6301 TTCTAGAAAG TCGACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT 6361 CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT 6421 GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT 6481 CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT 6541 GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC 6601 GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG 6661 ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC 6721 TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC 6781 GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG 6841 CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC 6901 CCGCGTCGAC TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA 6961 AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT CTGCTTTTTG 7021 CTTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG 7081 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC 7141 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA 7201 TCTCTAGCAG TACGTATAGT AGTTCATGTC ATCTTATTAT TCAGTATTTA TAACTTGCAA 7261 AGAAATGAAT ATCAGAGAGT GAGAGGAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 7321 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 7381 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGCT CTAGCTATCC CGCCCCTAAC 7441 TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT 7501 AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA 7561 GTGAGGAGGC TTTTTTGGAG GCCTAGGGAC GTACCCAATT CGCCCTATAG TGAGTCGTAT 7621 TACGCGCGCT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC 7681 CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC 7741 CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGGA CGCGCCCTGT 7801 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 7861 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 7921 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 7981 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 8041 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 8101 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 8161 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 8221 AACAAAATAT TAACGCTTAC AATTTAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC 8281 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT 8341 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG 8401 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG 8461 TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC 8521 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA 8581 CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC 8641 TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA 8701 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG 8761 ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT 8821 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG 8881 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC 8941 GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA 9001 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA 9061 TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC 9121 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG 9181 ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT 9241 CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA 9301 GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT 9361 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT 9421 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT 9481 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA 9541 TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG 9601 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA 9661 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG 9721 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA 9781 GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA 9841 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA 9901 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT 9961 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC 10021 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT 10081 CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA 10141 CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC 10201 TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG 10261 CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT 10321 TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA 10381 CAGGAAACAG CTATGACCAT GATTACGCCA AGCGCGCAAT TAACCCTCAC TAAAGGGAAC 10441 AAAAGCTGGA GCTGCAAGCT TAATGTAGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.