pLenti-U6-TNRC18-sgRNA1-Cas9-Puro plasmid

PVT109967

pLenti-U6-TNRC18-sgRNA1-Cas9-Puro plasmid Information

Plasmid name: pLenti-U6-TNRC18(human)-sgRNA1-Cas9-Puro
Alias: CGGTCATCCGCTCGCTCAAG
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene knockout
Fragment type: Cas9
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: High
Induction:

pLenti-U6-TNRC18-sgRNA1-Cas9-Puro plasmid Description

pLenti-U6-TNRC18-sgRNA1-Cas9-Puro It is a plasmid suitable for gene knockout in mammalian cells, and is also suitable for lentivirus packaging.

pLenti-U6-TNRC18-sgRNA1-Cas9-Puro plasmid Sequence

1 TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC 61 CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT 121 TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT 181 GGGAGGTCTA TATAAGCAGC GCGTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC 241 TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG 301 CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC 361 CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA 421 AAGCGAAAGG GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA 481 CGGCAAGAGG CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT 541 AGAAGGAGAG AGATGGGTGC GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT 601 GGGAAAAAAT TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT 661 GGGCAAGCAG GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG 721 GCTGTAGACA AATACTGGGA CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA 781 GATCATTATA TAATACAGTA GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG 841 ACACCAAGGA AGCTTTAGAC AAGATAGAGG AAGAGCAAAA CAAAAGTAAG ACCACCGCAC 901 AGCAAGCGGC CGCTGATCTT CAGACCTGGA GGAGGAGATA TGAGGGACAA TTGGAGAAGT 961 GAATTATATA AATATAAAGT AGTAAAAATT GAACCATTAG GAGTAGCACC CACCAAGGCA 1021 AAGAGAAGAG TGGTGCAGAG AGAAAAAAGA GCAGTGGGAA TAGGAGCTTT GTTCCTTGGG 1081 TTCTTGGGAG CAGCAGGAAG CACTATGGGC GCAGCGTCAA TGACGCTGAC GGTACAGGCC 1141 AGACAATTAT TGTCTGGTAT AGTGCAGCAG CAGAACAATT TGCTGAGGGC TATTGAGGCG 1201 CAACAGCATC TGTTGCAACT CACAGTCTGG GGCATCAAGC AGCTCCAGGC AAGAATCCTG 1261 GCTGTGGAAA GATACCTAAA GGATCAACAG CTCCTGGGGA TTTGGGGTTG CTCTGGAAAA 1321 CTCATTTGCA CCACTGCTGT GCCTTGGAAT GCTAGTTGGA GTAATAAATC TCTGGAACAG 1381 ATTTGGAATC ACACGACCTG GATGGAGTGG GACAGAGAAA TTAACAATTA CACAAGCTTA 1441 ATACACTCCT TAATTGAAGA ATCGCAAAAC CAGCAAGAAA AGAATGAACA AGAATTATTG 1501 GAATTAGATA AATGGGCAAG TTTGTGGAAT TGGTTTAACA TAACAAATTG GCTGTGGTAT 1561 ATAAAATTAT TCATAATGAT AGTAGGAGGC TTGGTAGGTT TAAGAATAGT TTTTGCTGTA 1621 CTTTCTATAG TGAATAGAGT TAGGCAGGGA TATTCACCAT TATCGTTTCA GACCCACCTC 1681 CCAACCCCGA GGGGACCCGA CAGGCCCGAA GGAATAGAAG AAGAAGGTGG AGAGAGAGAC 1741 AGAGACAGAT CCATTCGATT AGTGAACGGA TCGGCACTGC GTGCGCCAAT TCTGCAGACA 1801 AATGGCAGTA TTCATCCACA ATTTTAAAAG AAAAGGGGGG ATTGGGGGGT ACAGTGCAGG 1861 GGAAAGAATA GTAGACATAA TAGCAACAGA CATACAAACT AAAGAATTAC AAAAACAAAT 1921 TACAAAAATT CAAAATTTTC GGGTTTATTA CAGGGACAGC AGAGATCCAG TTTGGTTAAT 1981 TAAGGTACCG AGGGCCTATT TCCCATGATT CCTTCATATT TGCATATACG ATACAAGGCT 2041 GTTAGAGAGA TAATTAGAAT TAATTTGACT GTAAACACAA AGATATTAGT ACAAAATACG 2101 TGACGTAGAA AGTAATAATT TCTTGGGTAG TTTGCAGTTT TAAAATTATG TTTTAAAATG 2161 GACTATCATA TGCTTACCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 2221 TGGAAAGGAC GAAACACCGC GGTCATCCGC TCGCTCAAGG TTTTAGAGCT AGAAATAGCA 2281 AGTTAAAATA AGGCTAGTCC GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGCTTTTT 2341 TGAATTCGCT AGCTAGGTCT TGAAAGGAGT GGGAATTGGC TCCGGTGCCC GTCAGTGGGC 2401 AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGATCCGG 2461 TGCCTAGAGA AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT 2521 TTTTCCCGAG GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT 2581 TCGCAACGGG TTTGCCGCCA GAACACAGGA CCGGTTCTAG AGCGCTGCCA CCATGGACAA 2641 GAAGTACAGC ATCGGCCTGG ACATCGGCAC CAACTCTGTG GGCTGGGCCG TGATCACCGA 2701 CGAGTACAAG GTGCCCAGCA AGAAATTCAA GGTGCTGGGC AACACCGACC GGCACAGCAT 2761 CAAGAAGAAC CTGATCGGAG CCCTGCTGTT CGACAGCGGC GAAACAGCCG AGGCCACCCG 2821 GCTGAAGAGA ACCGCCAGAA GAAGATACAC CAGACGGAAG AACCGGATCT GCTATCTGCA 2881 AGAGATCTTC AGCAACGAGA TGGCCAAGGT GGACGACAGC TTCTTCCACA GACTGGAAGA 2941 GTCCTTCCTG GTGGAAGAGG ATAAGAAGCA CGAGCGGCAC CCCATCTTCG GCAACATCGT 3001 GGACGAGGTG GCCTACCACG AGAAGTACCC CACCATCTAC CACCTGAGAA AGAAACTGGT 3061 GGACAGCACC GACAAGGCCG ACCTGCGGCT GATCTATCTG GCCCTGGCCC ACATGATCAA 3121 GTTCCGGGGC CACTTCCTGA TCGAGGGCGA CCTGAACCCC GACAACAGCG ACGTGGACAA 3181 GCTGTTCATC CAGCTGGTGC AGACCTACAA CCAGCTGTTC GAGGAAAACC CCATCAACGC 3241 CAGCGGCGTG GACGCCAAGG CCATCCTGTC TGCCAGACTG AGCAAGAGCA GACGGCTGGA 3301 AAATCTGATC GCCCAGCTGC CCGGCGAGAA GAAGAATGGC CTGTTCGGAA ACCTGATTGC 3361 CCTGAGCCTG GGCCTGACCC CCAACTTCAA GAGCAACTTC GACCTGGCCG AGGATGCCAA 3421 ACTGCAGCTG AGCAAGGACA CCTACGACGA CGACCTGGAC AACCTGCTGG CCCAGATCGG 3481 CGACCAGTAC GCCGACCTGT TTCTGGCCGC CAAGAACCTG TCCGACGCCA TCCTGCTGAG 3541 CGACATCCTG AGAGTGAACA CCGAGATCAC CAAGGCCCCC CTGAGCGCCT CTATGATCAA 3601 GAGATACGAC GAGCACCACC AGGACCTGAC CCTGCTGAAA GCTCTCGTGC GGCAGCAGCT 3661 GCCTGAGAAG TACAAAGAGA TTTTCTTCGA CCAGAGCAAG AACGGCTACG CCGGCTACAT 3721 TGACGGCGGA GCCAGCCAGG AAGAGTTCTA CAAGTTCATC AAGCCCATCC TGGAAAAGAT 3781 GGACGGCACC GAGGAACTGC TCGTGAAGCT GAACAGAGAG GACCTGCTGC GGAAGCAGCG 3841 GACCTTCGAC AACGGCAGCA TCCCCCACCA GATCCACCTG GGAGAGCTGC ACGCCATTCT 3901 GCGGCGGCAG GAAGATTTTT ACCCATTCCT GAAGGACAAC CGGGAAAAGA TCGAGAAGAT 3961 CCTGACCTTC CGCATCCCCT ACTACGTGGG CCCTCTGGCC AGGGGAAACA GCAGATTCGC 4021 CTGGATGACC AGAAAGAGCG AGGAAACCAT CACCCCCTGG AACTTCGAGG AAGTGGTGGA 4081 CAAGGGCGCT TCCGCCCAGA GCTTCATCGA GCGGATGACC AACTTCGATA AGAACCTGCC 4141 CAACGAGAAG GTGCTGCCCA AGCACAGCCT GCTGTACGAG TACTTCACCG TGTATAACGA 4201 GCTGACCAAA GTGAAATACG TGACCGAGGG AATGAGAAAG CCCGCCTTCC TGAGCGGCGA 4261 GCAGAAAAAG GCCATCGTGG ACCTGCTGTT CAAGACCAAC CGGAAAGTGA CCGTGAAGCA 4321 GCTGAAAGAG GACTACTTCA AGAAAATCGA GTGCTTCGAC TCCGTGGAAA TCTCCGGCGT 4381 GGAAGATCGG TTCAACGCCT CCCTGGGCAC ATACCACGAT CTGCTGAAAA TTATCAAGGA 4441 CAAGGACTTC CTGGACAATG AGGAAAACGA GGACATTCTG GAAGATATCG TGCTGACCCT 4501 GACACTGTTT GAGGACAGAG AGATGATCGA GGAACGGCTG AAAACCTATG CCCACCTGTT 4561 CGACGACAAA GTGATGAAGC AGCTGAAGCG GCGGAGATAC ACCGGCTGGG GCAGGCTGAG 4621 CCGGAAGCTG ATCAACGGCA TCCGGGACAA GCAGTCCGGC AAGACAATCC TGGATTTCCT 4681 GAAGTCCGAC GGCTTCGCCA ACAGAAACTT CATGCAGCTG ATCCACGACG ACAGCCTGAC 4741 CTTTAAAGAG GACATCCAGA AAGCCCAGGT GTCCGGCCAG GGCGATAGCC TGCACGAGCA 4801 CATTGCCAAT CTGGCCGGCA GCCCCGCCAT TAAGAAGGGC ATCCTGCAGA CAGTGAAGGT 4861 GGTGGACGAG CTCGTGAAAG TGATGGGCCG GCACAAGCCC GAGAACATCG TGATCGAAAT 4921 GGCCAGAGAG AACCAGACCA CCCAGAAGGG ACAGAAGAAC AGCCGCGAGA GAATGAAGCG 4981 GATCGAAGAG GGCATCAAAG AGCTGGGCAG CCAGATCCTG AAAGAACACC CCGTGGAAAA 5041 CACCCAGCTG CAGAACGAGA AGCTGTACCT GTACTACCTG CAGAATGGGC GGGATATGTA 5101 CGTGGACCAG GAACTGGACA TCAACCGGCT GTCCGACTAC GATGTGGACC ATATCGTGCC 5161 TCAGAGCTTT CTGAAGGACG ACTCCATCGA CAACAAGGTG CTGACCAGAA GCGACAAGAA 5221 CCGGGGCAAG AGCGACAACG TGCCCTCCGA AGAGGTCGTG AAGAAGATGA AGAACTACTG 5281 GCGGCAGCTG CTGAACGCCA AGCTGATTAC CCAGAGAAAG TTCGACAATC TGACCAAGGC 5341 CGAGAGAGGC GGCCTGAGCG AACTGGATAA GGCCGGCTTC ATCAAGAGAC AGCTGGTGGA 5401 AACCCGGCAG ATCACAAAGC ACGTGGCACA GATCCTGGAC TCCCGGATGA ACACTAAGTA 5461 CGACGAGAAT GACAAGCTGA TCCGGGAAGT GAAAGTGATC ACCCTGAAGT CCAAGCTGGT 5521 GTCCGATTTC CGGAAGGATT TCCAGTTTTA CAAAGTGCGC GAGATCAACA ACTACCACCA 5581 CGCCCACGAC GCCTACCTGA ACGCCGTCGT GGGAACCGCC CTGATCAAAA AGTACCCTAA 5641 GCTGGAAAGC GAGTTCGTGT ACGGCGACTA CAAGGTGTAC GACGTGCGGA AGATGATCGC 5701 CAAGAGCGAG CAGGAAATCG GCAAGGCTAC CGCCAAGTAC TTCTTCTACA GCAACATCAT 5761 GAACTTTTTC AAGACCGAGA TTACCCTGGC CAACGGCGAG ATCCGGAAGC GGCCTCTGAT 5821 CGAGACAAAC GGCGAAACCG GGGAGATCGT GTGGGATAAG GGCCGGGATT TTGCCACCGT 5881 GCGGAAAGTG CTGAGCATGC CCCAAGTGAA TATCGTGAAA AAGACCGAGG TGCAGACAGG 5941 CGGCTTCAGC AAAGAGTCTA TCCTGCCCAA GAGGAACAGC GATAAGCTGA TCGCCAGAAA 6001 GAAGGACTGG GACCCTAAGA AGTACGGCGG CTTCGACAGC CCCACCGTGG CCTATTCTGT 6061 GCTGGTGGTG GCCAAAGTGG AAAAGGGCAA GTCCAAGAAA CTGAAGAGTG TGAAAGAGCT 6121 GCTGGGGATC ACCATCATGG AAAGAAGCAG CTTCGAGAAG AATCCCATCG ACTTTCTGGA 6181 AGCCAAGGGC TACAAAGAAG TGAAAAAGGA CCTGATCATC AAGCTGCCTA AGTACTCCCT 6241 GTTCGAGCTG GAAAACGGCC GGAAGAGAAT GCTGGCCTCT GCCGGCGAAC TGCAGAAGGG 6301 AAACGAACTG GCCCTGCCCT CCAAATATGT GAACTTCCTG TACCTGGCCA GCCACTATGA 6361 GAAGCTGAAG GGCTCCCCCG AGGATAATGA GCAGAAACAG CTGTTTGTGG AACAGCACAA 6421 GCACTACCTG GACGAGATCA TCGAGCAGAT CAGCGAGTTC TCCAAGAGAG TGATCCTGGC 6481 CGACGCTAAT CTGGACAAAG TGCTGTCCGC CTACAACAAG CACCGGGATA AGCCCATCAG 6541 AGAGCAGGCC GAGAATATCA TCCACCTGTT TACCCTGACC AATCTGGGAG CCCCTGCCGC 6601 CTTCAAGTAC TTTGACACCA CCATCGACCG GAAGAGGTAC ACCAGCACCA AAGAGGTGCT 6661 GGACGCCACC CTGATCCACC AGAGCATCAC CGGCCTGTAC GAGACACGGA TCGACCTGTC 6721 TCAGCTGGGA GGCGACAAGC GACCTGCCGC CACAAAGAAG GCTGGACAGG CTAAGAAGAA 6781 GAAAGATTAC AAAGACGATG ACGATAAGGG ATCCGGCGCA ACAAACTTCT CTCTGCTGAA 6841 ACAAGCCGGA GATGTCGAAG AGAATCCTGG ACCGACCGAG TACAAGCCCA CGGTGCGCCT 6901 CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA 6961 CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA 7021 AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG 7081 CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA 7141 GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA 7201 AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGAGT 7261 CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC 7321 GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT 7381 CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC 7441 CTGGTGCATG ACCCGCAAGC CCGGTGCCTG AACGCGTTAA GTCGACAATC AACCTCTGGA 7501 TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG 7561 TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT 7621 CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG 7681 GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC 7741 CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA 7801 ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA 7861 TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC 7921 CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT 7981 TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA 8041 GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCGTCGA CTTTAAGACC AATGACTTAC 8101 AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT 8161 CACTCCCAAC GAAGACAAGA TCTGCTTTTT GCTTGTACTG GGTCTCTCTG GTTAGACCAG 8221 ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC 8281 TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA 8341 TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GGGCCCGTTT AAACCCGCTG 8401 ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC 8461 TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC 8521 ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA 8581 GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC 8641 TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC 8701 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT 8761 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG 8821 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA 8881 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT 8941 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG 9001 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC 9061 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG 9121 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 9181 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 9241 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 9301 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 9361 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA 9421 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 9481 TTCGGATCTG ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT 9541 AATACGACAA GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA 9601 CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG 9661 ACTTCGTGGA GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG 9721 CGGTCCAGGA CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG 9781 ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC 9841 CGGCCATGAC CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG 9901 CCGGCAACTG CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG 9961 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 10021 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 10081 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 10141 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 10201 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 10261 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 10321 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 10381 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 10441 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 10501 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 10561 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 10621 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 10681 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 10741 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 10801 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 10861 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 10921 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 10981 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 11041 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 11101 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 11161 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 11221 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 11281 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 11341 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 11401 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 11461 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 11521 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 11581 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 11641 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 11701 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 11761 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 11821 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 11881 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 11941 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 12001 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 12061 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 12121 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 12181 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 12241 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 12301 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 12361 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCGA CGGATCGGGA GATCTCCCGA 12421 TCCCCTATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATCT 12481 GCTCCCTGCT TGTGTGTTGG AGGTCGCTGA GTAGTGCGCG AGCAAAATTT AAGCTACAAC 12541 AAGGCAAGGC TTGACCGACA ATTGCATGAA GAATCTGCTT AGGGTTAGGC GTTTTGCGCT 12601 GCTTCGCGAT GTACGGGCCA GATATACGCG TTGACATTGA TTATTGACTA GTTATTAATA 12661 GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT 12721 TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT 12781 GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA 12841 TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC 12901 TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG 12961 GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.