pLKO.1-U6-Plxnb1-shRNA1-mCherry-Puro plasmid

PVT94880

pLKO.1-U6-Plxnb1-shRNA1-mCherry-Puro plasmid Information

Plasmid name: pLKO.1-U6-Plxnb1(mouse)-shRNA1-mCherry-Puro
Alias: GGTAGACCAGCTTGCTAATTA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLKO.1-U6-Plxnb1-shRNA1-mCherry-Puro plasmid Description

pLKO.1-U6-Plxnb1-shRNA1-mCherry-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLKO.1-U6-Plxnb1-shRNA1-mCherry-Puro plasmid Sequence

1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatccATGGT GAGCAAGGGC GAGGAGGATA 541 ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC TCCGTGAACG 601 GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG 661 CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCTC 721 AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC GACTACTTGA 781 AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG GACGGCGGCG 841 TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC AAGGTGAAGC 901 TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGACC ATGGGCTGGG 961 AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG ATCAAGCAGA 1021 GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT CAAGACCACC TACAAGGCCA 1081 AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT CAAGTTGGAC ATCACCTCCC 1141 ACAACGAGGA CTACACCATC GTGGAACAGT ACGAACGCGC CGAGGGCCGC CACTCCACCG 1201 GCGGCATGGA CGAGCTGTAC AAGGAATTTG GCAGCGGCCA GTGCACCAAC TATGCCCTGC 1261 TGAAACTGGC TGGCGATGTG GAAAGCAACC CCGGACCCat gaccgagtac aagcccacgg 1321 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg 1381 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg 1441 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 1501 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 1561 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 1621 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 1681 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 1741 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc 1801 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac 1861 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc 1921 gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca atgacttaca 1981 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc 2041 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 2101 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 2161 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 2221 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 2281 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 2341 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 2401 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 2461 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 2521 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 2581 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc 2641 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt 2701 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc 2761 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg 2821 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 2881 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 2941 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 3001 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 3061 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 3121 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 3181 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 3241 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt 3301 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 3361 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 3421 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 3481 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 3541 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 3601 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 3661 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 3721 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 3781 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 3841 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 3901 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 3961 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 4021 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 4081 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 4141 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 4201 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 4261 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 4321 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 4381 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 4441 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 4501 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 4561 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag 4621 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 4681 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 4741 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 4801 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 4861 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 4921 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 4981 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 5041 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 5101 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 5161 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 5221 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 5281 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 5341 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 5401 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg 5461 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc 5521 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga 5581 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg 5641 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga 5701 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg 5761 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 5821 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 5881 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa 5941 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg 6001 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag 6061 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg 6121 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg 6181 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc 6241 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga 6301 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac 6361 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag 6421 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga 6481 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa 6541 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt 6601 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag 6661 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca 6721 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc 6781 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact 6841 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat 6901 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat 6961 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga 7021 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat 7081 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact 7141 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc 7201 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag 7261 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg 7321 agcggccgcc cccttcaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg 7381 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt 7441 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg 7501 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt 7561 atatatcttg tggaaaggac gaaacACCGG GGTAGACCAG CTTGCTAATT Attcaagaga 7621 TAATTAGCAA GCTGGTCTAC CttttttGAA TTCtcgacct cgagacaaat ggcagtattc 7681 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta 7741 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa 7801 aattttcggg tttattacag ggacagcaga gatccacttt ggccgcggct cgaggggg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???