pLKO.1-U6-VHL-shRNA1-Puro plasmid

PVT109933

pLKO.1-U6-VHL-shRNA1-Puro plasmid Information

Plasmid name: pLKO.1-U6-VHL(human)-shRNA1-Puro
Alias: TATCACACTGCCAGTGTATAC
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene interference
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: High
Induction:

pLKO.1-U6-VHL-shRNA1-Puro plasmid Description

pLKO.1-U6-VHL-shRNA1-Puro It is a plasmid suitable for gene interference in mammalian cells, and is also suitable for lentivirus packaging.

pLKO.1-U6-VHL-shRNA1-Puro plasmid Sequence

1 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc 61 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 121 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 181 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 241 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 301 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 361 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 421 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac 481 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 541 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 601 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc 661 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 721 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 781 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg 841 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 901 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 961 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 1021 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 1081 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 1141 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 1201 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca 1261 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 1321 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 1381 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 1441 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 1501 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 1561 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 1621 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 1681 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 1741 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 1801 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 1861 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 1921 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 1981 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 2041 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 2101 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 2161 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 2221 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 2281 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 2341 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 2401 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 2461 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 2521 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 2581 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 2641 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 2701 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 2761 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 2821 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 2881 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 2941 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 3001 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 3061 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 3121 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 3181 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 3241 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 3301 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 3361 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 3421 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt 3481 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca 3541 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga 3601 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt 3661 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg 3721 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 3781 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 3841 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg 3901 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt 3961 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 4021 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 4081 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 4141 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 4201 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 4261 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 4321 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 4381 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga 4441 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 4501 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 4561 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct 4621 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 4681 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 4741 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 4801 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 4861 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa 4921 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 4981 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 5041 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 5101 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 5161 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 5221 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatc 5281 acgagactag cctcgagcgg ccgccccctt caccgagggc ctatttccca tgattccttc 5341 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa 5401 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc 5461 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt 5521 cgatttcttg gctttatata tcttgtggaa aggacgaaac ACCGGTATCA CACTGCCAGT 5581 GTATACttca agagaGTATA CACTGGCAGT GTGATATTTT TTGAATTCtc gacctcgaga 5641 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca 5701 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa 5761 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg 5821 cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg 5881 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt 5941 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc 6001 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac 6061 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc 6121 aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc 6181 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc 6241 cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt 6301 cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccac cggagcttac 6361 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt 6421 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga 6481 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 6541 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 6601 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 6661 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 6721 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 6781 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 6841 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 6901 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 6961 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcggt 7021 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 7081 ggggggac

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.