pLMF03 Plasmid
Catalog No.:
PVT50461
pLMF03 PlasmidAlias:Gene length:
Host:
riemerella anatipestifer
Use(s):
Protein expressionFragment Type:
ORF
Fragmented species:
Prokaryotic resistance:
Amp
Screening Markers:
Promoter:
replicon:
Copy Number:
Competent cells:
DH5a
Temperature:
37Degrees
Back Bones:
Forward primer:
Reverse primer:
Induction method:
Sequence:
aaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcttgggctatttaggcattagccctctttttaacctgacttaaaaaataattaaatcataagatattaaatatcaagtgattatgatttactatgctatacataatatcacccagtgttagaaaattcttgctatgctttttgtttgttttttttcattcgtgaacttttagaatacttttaaaataaactcaaatttataaactattgattttcaatattatacaaaaccaaacttattaacattaaacttatttctattacctttaaaataaactactaaaagacaaatctaccaatcctcggaactatttaataaataggaagttatataaaaataatgaacttttagaatactttattagtctttttagctccaaaaatgaacttttagaatactttttttgaacttttagaatacttttttaaacttgctttttagtaagttcattttttatatatttgtattctaaaagtgcattttatattcataactctctatatatggcaaaagtagataataaactaatgtttcagtcctatatttttactacggctaaatatgatttttcagtctatgaaaagcgtatactatataggcaaatagaaatagaacaggcattgctaaataatgaggcgttgaaagattgtatcaaaatagatactaacctatggggggataaaaaatatacaattccaatttctatgctgttagctaatgatgaagataaaaattattataaaattaaaaaagcattcaaagacctaaaatcaaagaacattgagtacgaagatgaaaaagaatacgcttgctttggtgttattgataagttttggattgataaatatggaagaaatgttacttggcaatctgacaaaagaattgttgaagcggtaatggattttacaagaggatggagaaaatatgaattgaagattgctatgcagtttgagactatttatgcaatgcgtttttacgaactgatagctaataaaaccaaaccaataacatatgaagtgtcatttcttgaaaaaatgtttcaattggaaaataagtacaggaaccctcagggtaatcttaatatcagtatgtttaaacgccgtgtattagaccccgctaaaaacgagttagataaatgttctccatacacttttgattatactctttcaaaggataaaaaaattatttctctaattcctattttccaagaacaatttgcagatgaaagtatgaagatgatcaaaatagaagaggaaaaagatatacactctgtattaactcaaagagaaattgatgtttttacaaacgaatttggttttacacaacaaggtttattaaacaactatgccctattcaaagattgtaaggctacacttccatcaaactattctttcttcctttttcaagagataagaaaaagtcggactaagaaaggaaaaatatctcctgcttatgtgattgggattataagaaatatgcttaaagatttcaaaaaagaacaacaataggcttatgaaaaaatatacaacaaaagaaatcgcagagctttttggcgtcagcgaacggacaatccagcgacacataacgacactaagcgacaacatatcaaataccgacagaaagggcttttcaataccagaagacattgttttctttcttgccgagcgacaccattacgacattttagcgacaacgaacgacaacgaacgacaggataaaaatgaagaatttcctcatattgaatacttcacagaagaggaataccaagagtttaaaaaaaggatcacagagtacccttttttgaaagagcaaattaaactgtctaatgaacatttggatactctaaaaacacaaatagaatatttcagactatcttacaataagcaattggatattcacgaaaaattaatcggagcgttccgagaaagaaactttattgaagctaaagaaaaggggatggataaatagctcctaataattaaagaagtattagaaaaaaaatgctatataaaaacttaatcgagctctatacgagatctgttccaatgcattggctgcaggtcgacatttcaaaaatttaacttaaaccactgatttacaaaaatgtttatttgcaaaactattttacaaagatattacaataatttttctagcacaaatttatttctcaccatatctttgccttgttcaagtgagtgaacagagtaacaaacaaaaaaagaaacaaatttaaaaaaattatttaaaattccatggctctagaactcgagcatcatcatcatcatcattgaactagtggagttgcttctcttttcgggagtggaaaactgaaagaactggaacgtgccaacgaaaaactgcaagacgaggtttcaaaacggaacaccaatattgaaaaattgcagagccaagtacagcagatgcagaaacagcatgatacgcaaatccacaatctcagagaaatgcacaggcaggaacttgacatgaaagaaaaagaactgtcacggctcgccagaatcatagacaaggctttcaggtggtttccgatgttcagggaaatgctgcgcatggaaaagttttgtgccatgctgggattctctaaagaaatgactgaaagtcttatagtcaaaaaagaagccctgaaatgtagcggtaaaatctattccgagcaacacaggcggaactttgatataaaggatgatattttaagggtggaaaatgaccctgacgatgaaagcaggctgaacctgacaataaacaggaagccgattgccgactggttcagggagcaatggcacaggcttagatatggagcaagagtgccgcaacaggaagaaagaaaaagtagaggattcaaattataatagaagcaatttgattagtaatctaaaagcactccgataacgattagagtgcttttagattgtttatcattaattatcaaagcaagtgcagtttaagattttactgaagtttgcattaataaagaatatactacagctgatatatgcgcaacatattgtgacgcttgtgatttatttcccttgaaatccttaacaaataccgctaaggtataactgatattattaggcagacatatataggcaacatcattgtgagctgcaagaacaccattttcattaacataacctgaacctgtcttatgcgctataacaaccccttctttatcaagaagtggagctgctatcctatctacacctgttttgcattcttttaacgtattcttaatgaaactttgtttctcatcatcgataagaccttcagtaaacaaacgattcatcaacattgcagcaccaagaggagatgtatagttagagtaagccttgttatggtcagccgacatttcctcttccgtataagctatctgaaaacttgaacgaggaatgagtgtggctataaaactatctgtttgagcgacattaaccatatccttaaacataaggttgcttgcattgttgtcactctgagtaagagtataacgcagcaaatctctcactgtcaatgatatgactggccctgaataatctttcagcataggactccaagtctttgggtcaagtttatccctatttatatttactaaggtatcaagtgaaattcctttattgtcaaagtcattacaaagagctaatgcctgatgaaccttaaacacactcatcataggataaacactcttattattgaccttaaccgtatctctgttattaacaataaccgccacaccaatttcgccaggacaagctgagacaatttgagaaatgctatcagtcaaaacatttgttaaaggaggatttgcgctatcttttgtcgctgatttatggaacaatgaaaataccaagatgaaaatgcaaactaaagctatactcaaaactacgatttgtttttttctgtttttttccatgttttatattatttatatttgtttgacgagaatatctttatttgccgacaaaggtacataactaaagtttcccacccaaataaatagatagaaaaataacagtttgtcgaattttctttgtaaattagtaatcgctaaagaactgattttggggatccccgggtaccgagctcgaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatgga
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Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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