pLV2-CMV-AKR1C1-3×FLAG-Puro plasmid

PVT97908

pLV2-CMV-AKR1C1-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-AKR1C1(human)-3×FLAG-Puro
Alias: NM_001353.6;2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB
Gene lenght: 969bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-AKR1C1-3×FLAG-Puro plasmid Description

pLV2-CMV-AKR1C1-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-AKR1C1-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggattc gaaatatcag tgtgtgaagc tgaatgatgg 2881 tcacttcatg cctgtcctgg gatttggcac ctatgcgcct gcagaggttc ctaaaagtaa 2941 agctttagag gccaccaaat tggcaattga agctggcttc cgccatattg attctgctca 3001 tttatacaat aatgaggagc aggttggact ggccatccga agcaagattg cagatggcag 3061 tgtgaagaga gaagacatat tctacacttc aaagctttgg tgcaattccc atcgaccaga 3121 gttggtccga ccagccttgg aaaggtcact gaaaaatctt caattggatt atgttgacct 3181 ctaccttatt cattttccag tgtctgtaaa gccaggtgag gaagtgatcc caaaagatga 3241 aaatggaaaa atactatttg acacagtgga tctctgtgcc acatgggagg ccgtggagaa 3301 gtgtaaagat gcaggattgg ccaagtccat cggggtgtcc aacttcaacc gcaggcagct 3361 ggagatgatc ctcaacaagc cagggctcaa gtacaagcct gtctgcaacc aggtggaatg 3421 tcatccttac ttcaaccaga gaaaactgct ggatttctgc aagtcaaaag acattgttct 3481 ggttgcctat agtgctctgg gatcccaccg agaagaacca tgggtggacc cgaactcccc 3541 ggtgctcttg gaggacccag tcctttgtgc cttggcaaaa aagcacaagc gaaccccagc 3601 cctgattgcc ctgcgctacc agctacagcg tggggttgtg gtcctggcca agagctacaa 3661 tgagcagcgc atcagacaga acgtgcaggt gtttgaattc cagttgactt cagaggagat 3721 gaaagccata gatggcctaa acagaaatgt gcgatatttg acccttgata tttttgctgg 3781 cccccctaat tatccatttt ctgatgaata tggatccGAC TACAAAGACC ATGACGGTGA 3841 TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGtagtcta gataattcta 3901 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct 3961 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag 4021 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt 4081 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc 4141 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct 4201 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag 4261 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc 4321 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc 4381 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc 4441 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 4501 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 4561 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 4621 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 4681 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 4741 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 4801 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 4861 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 4921 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 4981 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct ggaacaatca 5041 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 5101 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 5161 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 5221 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 5281 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 5341 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 5401 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 5461 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 5521 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 5581 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc 5641 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct 5701 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag 5761 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 5821 aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg 5881 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc 5941 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta 6001 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg 6061 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac 6121 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag 6181 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga 6241 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga 6301 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 6361 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 6421 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 6481 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg 6541 acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 6601 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6661 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6721 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6781 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6841 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6901 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6961 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 7021 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 7081 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 7141 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 7201 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7261 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7321 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7381 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 7441 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7501 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7561 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7621 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7681 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7741 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7801 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7861 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7921 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7981 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 8041 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 8101 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 8161 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 8221 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8281 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8341 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8401 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8461 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8521 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 8581 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8641 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8701 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 8761 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 8821 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 8881 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt 8941 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt 9001 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac 9061 ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???