pLV2-CMV-Aldh18a1-EGFP-Puro plasmid

PVT101066

pLV2-CMV-Aldh18a1-EGFP-Puro plasmid Information

Plasmid name: pLV2-CMV-Aldh18a1(rat)-EGFP-Puro
Alias: NM_001108524.2;Pycs
Gene lenght: 2385bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: pEGFP-N-3'
Copy number: High
Induction:

pLV2-CMV-Aldh18a1-EGFP-Puro plasmid Description

pLV2-CMV-Aldh18a1-EGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-Aldh18a1-EGFP-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgttgag acatgtgcaa cgctccgggc tccagccctt 2881 cagccaacgt ctgctaccgt gggtccagtc catagctctg cccagatctc atcgcgtcca 2941 gccttcagct attagacatg ttcgttcctg gagcaacatc ccctttatca cggtacccct 3001 cagtcgtgct catggcaagc cctttgccca ccgaagcgag ctgaagcacg ccaagagaat 3061 tgtagtgaaa ctcggcagtg ccgtggtgac cagaggagat gagtgtggcc tggcactggg 3121 gcgcctggcg tctattgtcg aacaggtctc ggtgctgcag aatcagggcc gagagatgat 3181 gctggtgacc agtggagcgg tcgccttcgg caagcagcgc ctgcgccacg agatccttct 3241 gtctcagagc gtgcggcaag ccctgcactc aggacagaac catctgaagg agatggcaat 3301 tccagtctta gaggcccgag cctgcgcagc cgctggacag agtgggctga tggccttgta 3361 cgaggccatg tttacacagt acagcatctg tgctgcccag attttggtga ccaacctgga 3421 tttccacgac gagcagaagc gcagaaatct caacggcaca ctacacgagc tgcttcggat 3481 gaacatcgtc cccattgtca acaccaacga cgccgttgtc cccccagctg agcccaacag 3541 tgatcttcag ggggtaaatg tgattagcgt taaggacaac gatagcctag ctgcccgtct 3601 ggccgtggag atgaaaactg acctcctgat tgtcctctca gatgtggaag gcctctttga 3661 cagcccccca ggctcagatg atgcaaaact cattgatata ttctacccgg gagatcagca 3721 gtctgtgaca tttggaacca agtctagagt gggcctaggg ggcatggaag ccaaggtgaa 3781 agccgccctc tgggctttgc aaggtggcac ttctgtcgtc attgccaacg gaacccaccc 3841 caaggtgtct gggcacgtca tcacagacat cgtggaggga aagaaagttg gcaccttctt 3901 ttcagaagta aagcctgctg gccctacggt ggagcagcag ggggagatgg ctcggtctgg 3961 agggagaatg ctggccacct tagagcctga acagagagct gaaattatca atcatctggc 4021 tgacctgctg acggaccagc gggaagagat cctgttagcc aacaaaaaag acttggagga 4081 agcagaggga agacttgcag gccccctgct gaagcgtctc agcctctcca cgtctaaact 4141 gaacagcctg gccatcgggc tgcggcagat cgcagcctcc tctcaggaga gcgtaggccg 4201 tgtcctgcgc cggactcgga ttgccaaaaa cctggagtta gaacaagtga ctgtcccaat 4261 aggtgtttta ctggtgatct ttgagtctcg tcctgactgt ctaccccagg tggcagcctt 4321 ggccattgcg agtggcaacg gcttgttgct caaaggcggg aaggaggcta tgcacagcaa 4381 ccgcattctt cacctcctga cccaggaggc cctctccatc catggagtca aggaggccat 4441 acagctggta aacaccagag aagaagtgga ggatctctgc cgcctggaca aaataattga 4501 cctgatcatc cctcgaggct cctcccagct ggtcagagac atccagaaag ctgccaaggg 4561 gatcccagtg atgggccaca gcgaagggat ctgccacatg tacgtggatt ctgaagccag 4621 tgtggacaag gtcacccgac ttgtcagaga ctccaaatgt gagtacccag ctgcctgtaa 4681 cgccctggag actttgctca tccaccgaga tctgctgaga acacccttat ttgaccagat 4741 cattgacatg ctcagagtgg aacaggtaaa aattcacgca ggccccaagt ttgcctccta 4801 cctgaccttc agcccctcag aagtgaagtc actccggaca gagtacgggg acctggaagt 4861 gtgcatcgaa gtggtggaca gcgtccagga agccattgat cacatccata agtacggcag 4921 ctcccacaca gatgtcatcg tcaccgagaa cgagaaaaca gccgagttct tcctccagca 4981 tgtggacagt gcctgcgtgt tctggaacgc cagtacccgc ttctctgatg gctaccgatt 5041 tggactggga gctgaagtag gaatcagtac atcacgaatc catgcccggg gaccagtagg 5101 actcgaggga ttgctaacta ctaagtggtt gcttcgaggg caagaccacg tggtctcaga 5161 cttctcagag cacggaaggc taaagtatct tcacgagaac ctccctgttc cccagaggca 5221 tttcagcggt ggaggcggat caggatccgc caccatggtg agcaagggcg aggagctgtt 5281 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 5341 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 5401 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt 5461 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 5521 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 5581 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 5641 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 5701 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 5761 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 5821 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag 5881 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 5941 gatcactctc ggcatggacg agctgtacaa gtagtctaga taattctacc gggtagggga 6001 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg 6061 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc 6121 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc 6181 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag 6241 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg 6301 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg 6361 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc 6421 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc 6481 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc 6541 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 6601 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 6661 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 6721 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 6781 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 6841 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 6901 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 6961 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 7021 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 7081 tggtgcatga cccgcaagcc cggtgcctga ccgcgtctgg aacaatcaac ctctggatta 7141 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 7201 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 7261 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 7321 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 7381 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 7441 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 7501 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg 7561 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 7621 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 7681 gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta 7741 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg 7801 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga 7861 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg 7921 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca 7981 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg 8041 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat 8101 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag 8161 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag 8221 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct 8281 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc 8341 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc 8401 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 8461 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 8521 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 8581 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg 8641 ttttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 8701 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 8761 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 8821 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 8881 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 8941 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 9001 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 9061 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 9121 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 9181 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 9241 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 9301 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 9361 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 9421 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 9481 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 9541 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 9601 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 9661 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 9721 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 9781 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 9841 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 9901 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 9961 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 10021 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 10081 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 10141 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 10201 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 10261 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 10321 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 10381 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 10441 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 10501 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 10561 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 10621 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 10681 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 10741 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 10801 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg 10861 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 10921 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 10981 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac 11041 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa 11101 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttttt 11161 aaagaaattg tatttgttaa atatgtacta caaacttagt agt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???