pLV2-CMV-CUEDC1-3×HA-Puro plasmid

PVT110322

pLV2-CMV-CUEDC1-3×HA-Puro plasmid Information

Plasmid name: pLV2-CMV-CUEDC1(human)-3×HA-Puro
Alias: NM_001271875.2
Gene lenght: 1158
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-CUEDC1-3×HA-Puro plasmid Description

pLV2-CMV-CUEDC1-3×HA-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV2-CMV-CUEDC1-3×HA-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgaccag cctgttccgc cggagcagca gcggcagcgg 2881 cgggggtggc accgccgggg cacgcggggg cgggggaggc acggccgccc cccaggagct 2941 caacaacagc cggcctgccc gccaggtgcg ccgcctggag ttcaaccagg ccatggacga 3001 cttcaagacc atgttcccca acatggatta cgacatcatc gaatgcgtgc tgcgcgccaa 3061 cagcggcgct gtggacgcca ccatcgacca gctgctgcag atgaacctgg agggcggtgg 3121 cagcagcggc ggcgtctatg aggacagctc cgactcggag gacagcatcc ccccggagat 3181 cttggaaagg actttggaac ctgatagctc ggatgaagag cccccacctg tgtactcccc 3241 gccagcctac cacatgcacg tgttcgaccg gccctaccct ctggctcccc cgactccgcc 3301 tccccgtatc gacgcgctgg gctctggagc ccctacaagc cagagacgct atcggaactg 3361 gaacccacca ctgctgggca accttccgga tgactttctc cgcatcctgc cccagcagct 3421 ggacagcata cagggtaacg ctgggggccc caagcctggg agtggagagg gatgtccacc 3481 tgccatggct gggccagggc ccggagacca ggagagccgc tggaagcagt acctggagga 3541 cgagaggatc gcgcttttcc tgcagaacga ggagttcatg aaggagctgc aacggaaccg 3601 cgacttcctc ctcgctctgg agagagatcg attgaaatac gaatcccaga aatctaaatc 3661 cagcagcgtg gctgtcggaa acgactttgg cttttcctct cctgtcccag gaactggcga 3721 cgccaacccc gctgtgtctg aagatgcctt attcagggac aagctgaaac acatgggaaa 3781 gtccacccgg aggaaactgt ttgaacttgc ccgagccttc tcagagaaga ccaaaatgag 3841 gaagtcaaag aggaaacact tgttgaagca tcagtcgctg ggggctgccg cgtcaacagc 3901 caacctcctg gatgatgtgg agggccacgc gtgtgatgaa gacttccggg gcaggcgtca 3961 ggaggcaccc aaggtggagg aaggcctgcg agaaggacag ggatccTACC CATACGACGT 4021 ACCTGATTAT GCAACTTATC CTTATGATGT ACCTGATTAT GCTAGCTATC CTTATGATGT 4081 CCCAGACTAC GCATAGtcta gataattcta ccgggtaggg gaggcgcttt tcccaaggca 4141 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg 4201 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc 4261 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc 4321 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag 4381 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc 4441 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg 4501 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca 4561 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag 4621 cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 4681 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 4741 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 4801 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 4861 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 4921 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 4981 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 5041 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 5101 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 5161 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 5221 cccggtgcct gaccgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt 5281 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 5341 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 5401 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 5461 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 5521 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 5581 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 5641 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 5701 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 5761 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg 5821 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc 5881 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag 5941 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca 6001 gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc 6061 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat 6121 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac 6181 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc 6241 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg 6301 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc 6361 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg 6421 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct 6481 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 6541 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 6601 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 6661 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 6721 gaaatgaata tcagagagtg agaggccttg acattgctag cgttttaccg tcgacctcta 6781 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 6841 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 6901 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 6961 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 7021 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 7081 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 7141 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 7201 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 7261 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 7321 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 7381 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 7441 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 7501 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 7561 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 7621 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 7681 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 7741 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 7801 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 7861 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 7921 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 7981 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 8041 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 8101 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 8161 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 8221 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 8281 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 8341 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 8401 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 8461 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 8521 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 8581 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 8641 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 8701 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 8761 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 8821 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 8881 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 8941 gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat 9001 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 9061 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata 9121 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat 9181 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga 9241 aattgtattt gttaaatatg tactacaaac ttagtagttt ttaaagaaat tgtatttgtt 9301 aaatatgtac tacaaactta gtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.