pLV2-CMV-Gstk1-3×FLAG-IRES-mCherry plasmid

PVT109864

pLV2-CMV-Gstk1-3×FLAG-IRES-mCherry plasmid Information

Plasmid name: pLV2-CMV-Gstk1(mouse)-3×FLAG-IRES-mCherry
Alias: NM_029555.2;0610025I19Rik; DsbA-L
Gene lenght: 3192
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-Gstk1-3×FLAG-IRES-mCherry plasmid Description

pLV2-CMV-Gstk1-3×FLAG-IRES-mCherry It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV2-CMV-Gstk1-3×FLAG-IRES-mCherry plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgggg 2821 ccggcgccgc gcatcctgga actcttctac gacgtgctgt ccccgtactc ctggctgggc 2881 tttgaggtcc tatgcagata ccaacacctc tggaatataa agctgcagtt gcggcccact 2941 ttaatcgctg ggatcatgaa agacagcgga aaccaaccac ctgctatggt tccccgaaaa 3001 ggccagtaca tcttcaaaga gattcctctc ctgaagcagt tcttccaggt tcccctcaac 3061 atacccaagg atttctttgg tgagactgtg aagaaaggaa gtataaatgc catgcgcttc 3121 ctcaccactg taagcatgga gcaaccagag atgctggaga aggtgtccag agagatatgg 3181 atgcgtgtat ggtctcgaga tgaagatatc acggagtatc agagcattct ggctgcagca 3241 gtgaaggctg gaatgtccac agcgcaagcc caacactttc tggagaagat ctccacacaa 3301 caggtgaaga acaagctcat tgagaacacg gatgcagcct gcaaatatgg ggcctttggg 3361 ctacccacca ctgttgccca tgtggatggt aaaacctaca tgttatttgg gtctgaccgc 3421 ttggagttgc tagcttacct gctaggagag aagtggatgg gccctgtacc cccaactgcg 3481 aatgccagac ttCTCGAGGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC 3541 GACTACAAGG ATGACGATGA CAAGTAGtga actagttcta gagcggccgc ggatcccgcc 3601 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg 3661 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga 3721 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa 3781 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa 3841 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct 3901 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg 3961 ttgtgagttg gatagttgtg gaaagagtca aatggctcac ctcaagcgta ttcaacaagg 4021 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca 4081 catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga 4141 cgtggttttc ctttgaaaaa cacgatgata atatggtgag caagggcgag gaggataaca 4201 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc 4261 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca 4321 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt 4381 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc 4441 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg 4501 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc 4561 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg 4621 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc 4681 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga 4741 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca 4801 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg 4861 gcatggacga gctgtacaag tgaacgcgtc tggaacaatc aacctctgga ttacaaaatt 4921 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 4981 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 5041 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 5101 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 5161 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 5221 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 5281 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg 5341 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 5401 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 5461 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac 5521 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag 5581 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga 5641 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc 5701 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct 5761 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg 5821 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag 5881 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag 5941 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc 6001 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact 6061 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat 6121 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag 6181 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 6241 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 6301 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 6361 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg 6421 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 6481 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 6541 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 6601 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 6661 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 6721 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 6781 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 6841 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 6901 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 6961 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 7021 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 7081 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 7141 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 7201 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 7261 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 7321 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 7381 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 7441 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 7501 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 7561 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 7621 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 7681 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 7741 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 7801 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 7861 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 7921 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 7981 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 8041 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 8101 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 8161 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 8221 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 8281 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 8341 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 8401 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 8461 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 8521 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 8581 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc 8641 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 8701 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 8761 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac 8821 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc 8881 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.