pLV2-CMV-mCherry-TRIM33-Puro plasmid

PVT110659

pLV2-CMV-mCherry-TRIM33-Puro plasmid Information

Plasmid name: pLV2-CMV-mCherry-TRIM33(human)-Puro
Alias: NM_015906.4;DDH4; ECTO; PTC7; RFG7; TF1G; TIF1G;TIF1GAMMA; TIFGAMMA
Gene lenght: 3384
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-mCherry-TRIM33-Puro plasmid Description

pLV2-CMV-mCherry-TRIM33-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV2-CMV-mCherry-TRIM33-Puro plasmid Sequence

1 TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA 61 CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC 121 TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA 181 ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG 241 AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG 301 AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG 361 CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT 421 CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA 481 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT 541 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC 601 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG 661 CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG 721 CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA 781 AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG 841 AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG 901 CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT 961 GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT 1021 CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA 1081 CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC 1141 AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG 1201 TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC 1261 AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG 1321 GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC 1381 CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC 1441 GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT 1501 GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA 1561 ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA 1621 GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT 1681 AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT 1741 GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA 1801 TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT 1861 ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT 1921 CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA 1981 CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG 2041 GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC 2101 AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG 2161 ACAGCAGAGA TCCAGTTTAT CGATAAGCTT GGGAGTTCCG CGTTACATAA CTTACGGTAA 2221 ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG 2281 TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT 2341 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG 2401 TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC 2461 CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC 2521 AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA 2581 TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA 2641 ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 2701 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC 2761 TCCATAGAAG ACACCGACTC TACTAGAGGA TCTACCGGTC GCCACCATGG TGAGCAAGGG 2821 CGAGGAGGAT AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC ACATGGAGGG 2881 CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG 2941 CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC CTGCCCTTCG CCTGGGACAT 3001 CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG CCGACATCCC 3061 CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA 3121 GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA 3181 CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAGAC 3241 CATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA 3301 GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCTGAGG TCAAGACCAC 3361 CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AACGTCAACA TCAAGTTGGA 3421 CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG TACGAACGCG CCGAGGGCCG 3481 CCACTCCACC GGCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCTC GAGgcggtgg 3541 aggcggatca ggaggcggtg gatctatggc ggaaaacaaa ggcggcggcg aggctgagag 3601 cggcggcggg ggcagcggca gcgcgccggt aactgccggg gccgccgggc ccgccgcgca 3661 ggaggcggag ccgcctctca ccgcggtgct ggtggaggag gaggaggagg aaggcggcag 3721 ggccggcgct gagggcggcg cggccgggcc cgacgacggg ggggtggccg cggcctcctc 3781 gggctcggcc caggctgctt catctcctgc ggcctcagtg ggcactggag ttgccggggg 3841 cgcagtatcg acgccggctc cagctccagc ctcggctccc gctccgggtc cctcggcagg 3901 gccgcctcct ggaccgccag cctcgctcct ggacacctgc gccgtgtgtc agcagagctt 3961 gcagagccgg cgtgaggcgg agcccaagct gctgccctgt cttcactcct tctgcctgcg 4021 ctgcctgccc gagccggagc gccagctcag cgtgcccatc ccggggggca gcaacggcga 4081 catccagcaa gttggtgtaa tacggtgccc agtatgccgc caagaatgca gacagataga 4141 ccttgtggat aattattttg tgaaagacac atctgaagct cctagcagtt ctgatgaaaa 4201 atcagaacag gtatgtacta gttgtgaaga caatgcaagt gcagttggct tttgtgtaga 4261 atgtggagag tggctatgta agacatgtat cgaagcacat caaagagtaa aatttactaa 4321 agatcacttg atcaggaaga aagaagatgt ctcagagtct gttggagcat ctggtcaacg 4381 ccctgttttc tgccctgtac acaaacaaga acagttgaaa cttttctgtg aaacatgtga 4441 tagattgaca tgtagagact gtcagctatt ggaacacaaa gaacataggt atcagttttt 4501 ggaagaagct tttcaaaatc agaagggtgc aattgagaat ctactggcga aacttcttga 4561 gaagaagaat tatgttcatt ttgcagctac tcaggtgcag aataggataa aagaagtaaa 4621 tgagactaac aaacgagtag aacaggaaat taaagtggcc attttcaccc ttatcaatga 4681 aattaataag aaaggaaaat ctctcttaca acagctagag aatgttacaa aggaaagaca 4741 gatgaagtta ctacagcagc agaatgacat cacaggcctt tcccggcagg tgaagcatgt 4801 tatgaacttc acaaattggg caattgcaag tggcagcagc acagcactac tatacagcaa 4861 gcgactgatt actttccagt tgcgtcatat tttgaaagca cggtgtgatc ctgtccctgc 4921 tgctaatgga gcaatacgtt tccattgtga tcccaccttc tgggcaaaga atgtagtcaa 4981 tttaggtaat ctagtaatag agagtaaacc agctcctggt tatactccta atgttgtagt 5041 tgggcaagtt cctccaggga caaaccacat tagtaaaacc cctggacaga ttaacttagc 5101 acagcttcga ctccagcaca tgcaacaaca agtatatgca cagaaacatc agcagttgca 5161 acagatgagg atgcagcaac caccagcacc tgtaccaact acaacaacaa caacacaaca 5221 gcatcctaga caagcagccc ctcagatgtt acaacaacag cctcctcgat tgatcagtgt 5281 gcaaacaatg caaagaggca acatgaactg tggagctttt caagcccatc agatgagact 5341 ggctcagaat gctgccagaa taccagggat acccaggcac agcggccctc aatattccat 5401 gatgcagcca cacctccaaa gacaacactc aaacccaggg catgctggac cctttcccgt 5461 agtatcggta cacaacacca caatcaaccc aacgagccct actacagcaa ctatggcaaa 5521 tgcaaaccga ggtcccacca gcccatctgt tacagcaata gagctaatcc cctcagttac 5581 caatccagaa aaccttccat cgctgccaga tattccaccc atacagttgg aagatgctgg 5641 ctcaagtagt ttagataatc tactaagtag atacatctca ggcagtcacc tacccccaca 5701 gcctacaagc accatgaatc cttctccagg tccctctgcc ctttctccgg gatcatcagg 5761 tttatccaat tctcacacac ctgtgagacc cccaagtact tctagtactg gcagtcgagg 5821 cagctgtggg tcatcaggaa gaactgctga gaagacaagt cttagtttca aatctgatca 5881 ggtgaaggtc aagcaagaac ctgggactga agatgaaata tgtagctttt caggaggtgt 5941 aaaacaagaa aaaacagagg atggcaggag gagtgcttgc atgttgagca gtcctgagag 6001 tagcttgaca ccacctctct caaccaacct gcatctagaa agtgaattgg atgcattggc 6061 aagcctggaa aaccatgtga aaattgaacc tgcagatatg aatgaaagct gcaaacagtc 6121 agggctcagc agccttgtta atggaaagtc cccaattcga agcctcatgc acaggtcggc 6181 aaggattgga ggagatggca acaataaaga tgatgaccca aatgaagact ggtgtgctgt 6241 ctgccaaaac ggaggagatc tcttgtgctg cgaaaaatgt ccaaaggtct ttcatctaac 6301 ttgtcatgtt ccaacactac ttagctttcc aagtggggac tggatatgca cattttgtag 6361 agatattgga aagccagaag ttgaatatga ttgtgataat ttgcaacata gtaagaaggg 6421 gaaaactgcg caggggttaa gccccgtgga ccaaaggaaa tgtgaacgtc ttctgcttta 6481 cctctattgc catgaattaa gtattgaatt ccaggagcct gttcctgctt cgataccaaa 6541 ctactataaa attataaaga aaccaatgga tttatccacc gtgaaaaaga agcttcagaa 6601 aaaacattcc caacactacc aaatcccgga tgactttgtg gccgatgtcc gtttgatctt 6661 caagaactgt gaaaggttta atgaaatgat gaaagttgtt caagtttatg cagacacaca 6721 agagattaat ttgaaggctg attcagaagt agctcaggca gggaaagcag ttgcattgta 6781 ctttgaagat aaactcacag agatctactc agacaggacc ttcgcacctt tgccagagtt 6841 tgagcaggaa gaggatgatg gtgaggtaac tgaggactct gatgaagact ttatacagcc 6901 ccgcagaaaa cgcctaaagt cagatgagag accagtacat ataaagtaaG GATCCACCGG 6961 ATCTAGATAA CTGATCATAA TTCTACCGGG TAGGGGAGGC GCTTTTCCCA AGGCAGTCTG 7021 GAGCATGCGC TTTAGCAGCC CCGCTGGGCA CTTGGCGCTA CACAAGTGGC CTCTGGCCTC 7081 GCACACATTC CACATCCACC GGTAGGCGCC AACCGGCTCC GTTCTTTGGT GGCCCCTTCG 7141 CGCCACCTTC TACTCCTCCC CTAGTCAGGA AGTTCCCCCC CGCCCCGCAG CTCGCGTCGT 7201 GCAGGACGTG ACAAATGGAA GTAGCACGTC TCACTAGTCT CGTGCAGATG GACAGCACCG 7261 CTGAGCAATG GAAGCGGGTA GGCCTTTGGG GCAGCGGCCA ATAGCAGCTT TGCTCCTTCG 7321 CTTTCTGGGC TCAGAGGCTG GGAAGGGGTG GGTCCGGGGG CGGGCTCAGG GGCGGGCTCA 7381 GGGGCGGGGC GGGCGCCCGA AGGTCCTCCG GAGGCCCGGC ATTCTGCACG CTTCAAAAGC 7441 GCACGTCTGC CGCGCTGTTC TCCTCTTCCT CATCTCCGGG CCTTTCGACC TGCAGCCCAA 7501 GCTTACCATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCAG 7561 GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA 7621 TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG 7681 GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC 7741 GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT 7801 GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC 7861 CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG 7921 TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC 7981 CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT 8041 CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG 8101 TGCCTGACCG CGTCTGGAAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG 8161 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT 8221 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC 8281 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT 8341 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA 8401 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT 8461 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA 8521 CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT 8581 GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC 8641 TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG 8701 CCTCCCCGCC TGGAATTAAT TCTGCAGTCG AGACCTAGAA AAACATGGAG CAATCACAAG 8761 TAGCAATACA GCAGCTACCA ATGCTGATTG TGCCTGGCTA GAAGCACAAG AGGAGGAGGA 8821 GGTGGGTTTT CCAGTCACAC CTCAGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT 8881 AGATCTTAGC CACTTTTTAA AAGAAAAGAG GGGACTGGAA GGGCTAATTC ACTCCCAACG 8941 AAGACAAGAT ATCCTTGATC TGTGGATCTA CCACACACAA GGCTACTTCC CTGATTAGCA 9001 GAACTACACA CCAGGGCCAG GGGTCAGATA TCCACTGACC TTTGGATGGT GCTACAAGCT 9061 AGTACCAGTT GAGCCAGATA AGGTAGAAGA GGCCAATAAA GGAGAGAACA CCAGCTTGTT 9121 ACACCCTGTG AGCCTGCATG GGATGGATGA CCCGGAGAGA GAAGTGTTAG AGTGGAGGTT 9181 TGACAGCCGC CTAGCATTTC ATCACGTGGC CCGAGAGCTG CATCCGGAGT ACTTCAAGAA 9241 CTGCTGATAT CGAGCTTGCT ACAAGGGACT TTCCGCTGGG GACTTTCCAG GGAGGCGTGG 9301 CCTGGGCGGG ACTGGGGAGT GGCGAGCCCT CAGATCCTGC ATATAAGCAG CTGCTTTTTG 9361 CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG 9421 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC 9481 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA 9541 TCTCTAGCAG TAGTAGTTCA TGTCATCTTA TTATTCAGTA TTTATAACTT GCAAAGAAAT 9601 GAATATCAGA GAGTGAGAGG CCTTGACATT GCTAGCGTTT TACCGTCGAC CTCTAGCTAG 9661 AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT 9721 CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC 9781 TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC 9841 CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT 9901 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA 9961 GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC 10021 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT 10081 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG 10141 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC 10201 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC 10261 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC 10321 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC 10381 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT 10441 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT 10501 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC 10561 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT 10621 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG 10681 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC 10741 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA 10801 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG 10861 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG 10921 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA 10981 GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG 11041 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA 11101 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC 11161 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA 11221 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG 11281 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT 11341 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC 11401 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG 11461 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG 11521 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT 11581 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA 11641 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA 11701 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 11761 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 11821 GTGCCACCTG ACGTCGACGG ATCGGGAGAT CAACTTGTTT ATTGCAGCTT ATAATGGTTA 11881 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG 11941 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCAACT GGATAACTCA 12001 AGCTAACCAA AATCATCCCA AACTTCCCAC CCCATACCCT ATTACCACTG CCAATTACCT 12061 GTGGTTTCAT TTACTCTAAA CCTGTGATTC CTCTGAATTA TTTTCATTTT AAAGAAATTG 12121 TATTTGTTAA ATATGTACTA CAAACTTAGT AGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.