pLV2-CMV-Myc-TRIP12-Hyg plasmid

PVT104160

pLV2-CMV-Myc-TRIP12-Hyg plasmid Information

Plasmid name: pLV2-CMV-Myc-TRIP12(human-toxic-opt)-Hyg
Alias: NM_001284214.2,MRD49/TRIP-12/TRIPC/ULF;
Gene lenght: 6120
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-Myc-TRIP12-Hyg plasmid Description

pLV2-CMV-Myc-TRIP12-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-Myc-TRIP12-Hyg plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggaa 2821 caaaaactca tctcagaaga ggatctgctc gagactagtt ctagaATGTC CAACCGGCCT 2881 AATAACAATC CAGGGGGGTC ACTGCGACGT TCACAGAGGA ACACTGCCGG GGCCCAACCA 2941 CAAGACGACT CAATAGGAGG AAGGTCACAT TTAGGGCAGG CAAAACATAA GGGATATAGC 3001 CCTCCTGAGA GTAGAAAATC TAATTCTAAG GCACCCAAAG TGCAGTCTAA TACTACTTCT 3061 GAACTGTCAA GGGGACACCT TTCTAAAAGA AGCTGCAGTT CATCATCTGC TGTGATAGTT 3121 CCACAACCAG AGGATCCAGA CAGAGCCAAT ACTTCAGAAA GACAAAAAAC GGGGCAGGTG 3181 CCTAAGAAAG ACAATTCTCG AGGAGTGAAG CGCAGTGCTA GTCCAGACTA CAACAGGACC 3241 AATTCTCCTA GCTCTGCAAA AAAACCAAAA GCACTTCAGC ATACTGAATC TCCCTCAGAA 3301 ACAAATAAGC CACATAGTAA GTCAAAGAAG AGACATTTAG ACCAGGAGCA ACAACTGAAA 3361 TCTGCACAAT CACCATCAAC AAGCAAGGCT CATACCAGGA AGAGTGGGGC CACTGGCGGT 3421 TCACGGAGTC AGAAAAGAAA AAGGACAGAG AGTTCTTGTG TAAAGAGTGG CTCCGGGTCT 3481 GAATCAACTG GTGCAGAAGA GAGATCTGCG AAACCTACCA AGCTGGCTTC AAAATCAGCC 3541 ACCTCAGCCA AAGCTGGGTG TAGCACCATC ACTGATTCTT CTTCTGCTGC CTCTACTTCC 3601 TCCTCGTCTT CTGCTGTAGC CTCGGCCTCC TCCACTGTAC CACCAGGTGC CAGAGTGAAA 3661 CAAGGAAAAG ATCAGAACAA GGCCAGGCGT TCCCGTTCAG CGTCCAGTCC CAGCCCCAGA 3721 AGAAGTAGCA GGGAAAAGGA ACAGAGTAAA ACTGGTGGCT CTTCAAAATT TGATTGGGCT 3781 GCTCGTTTCA GCCCTAAAGT TAGCCTTCCT AAAACAAAAC TGTCTCTTCC AGGGTCTTCT 3841 AAGTCAGAGA CATCAAAACC TGGACCTTCT GGATTACAGG CCAAATTAGC AAGTTTAAGA 3901 AAATCTACGA AGAAACGCAG TGAGTCTCCA CCTGCTGAGC TCCCCAGTTT GAGGCGGAGC 3961 ACACGCCAAA AGACCACGGG CTCCTGTGCT AGTACCAGTC GGCGAGGCTC TGGCCTGGGC 4021 AAAAGAGGAG CAGCTGAAGC TCGTCGACAG GAGAAAATGG CAGACCCTGA AAGCAACCAG 4081 GAGGCAGTAA ATTCTTCAGC TGCTCGGACA GATGAAGCTC CCCAAGGAGC TGCAGCCTCT 4141 AGTTCTGTTG CAGGGGCTGT TGGCATGACC ACCTCTGGGG AGAGTGAATC AGATGATTCC 4201 GAGATGGGAC GTTTGCAAGC TTTGTTAGAG GCAAGGGGTC TTCCCCCTCA CCTATTTGGT 4261 CCTCTTGGTC CTCGGATGTC ACAGCTTTTC CATAGAACAA TTGGAAGTGG AGCTAGTTCT 4321 AAGGCCCAGC AGCTACTACA AGGATTGCAA GCCAGTGATG AAAGTCAACA GCTTCAGGCA 4381 GTTATTGAGA TGTGTCAGTT ACTGGTCATG GGAAATGAGG AGACACTGGG AGGGTTTCCT 4441 GTCAAGAGTG TTGTTCCAGC TTTGATTACG TTACTTCAGA TGGAGCACAA TTTTGATATT 4501 ATGAACCATG CTTGTCGAGC CTTAACATAC ATGATGGAAG CACTTCCTCG ATCTTCTGCT 4561 GTTGTAGTAG ATGCTATTCC TGTCTTTTTA GAAAAGCTGC AAGTTATTCA GTGTATTGAT 4621 GTGGCAGAGC AGGCCTTGAC TGCCTTGGAG ATGTTGTCAC GGAGACATAG TAAAGCCATT 4681 CTACAGGCGG GTGGTTTGGC AGACTGCTTG CTGTACCTAG AATTCTTCAG CATAAATGCC 4741 CAAAGAAATG CATTAGCAAT TGCAGCTAAT TGCTGCCAGA GTATCACGCC AGATGAATTT 4801 CATTTTGTGG CAGATTCACT CCCATTGCTA ACCCAAAGGC TAACACATCA GGATAAAAAG 4861 TCAGTAGAAA GCACTTGCCT TTGTTTTGCA CGCCTAGTGG ACAACTTCCA GCATGAGGAG 4921 AATTTACTCC AGCAGGTTGC TTCCAAAGAT CTGCTTACAA ATGTTCAACA GCTGTTGGTA 4981 GTGACTCCAC CCATTTTAAG TTCTGGGATG TTCATAATGG TGGTGAGAAT GTTTAGCCTT 5041 ATGTGCTCAA ATTGCCCTAC CCTGGCTGTT CAGCTGATGA AGCAGAACAT CGCAGAGACC 5101 CTTCATTTCC TGTTGTGTGG CGCTTCCAAC GGCAGCTGCC AGGAGCAGAT CGATTTGGTG 5161 CCCCGCTCTC CACAGGAATT GTACGAGCTG ACTAGCCTTA TCTGTGAGCT GATGCCCTGT 5221 CTGCCAAAGG AGGGAATATT CGCGGTCGAC ACAATGCTGA AGAAAGGCAA TGCCCAGAAC 5281 ACTGATGGGG CTATCTGGCA ATGGAGAGAT GATAGAGGCT TGTGGCACCC GTATAACCGG 5341 ATCGATAGCC GCATAATCGA GCAGATCAAT GAGGATACCG GCACAGCCCG CGCCATACAG 5401 AGGAAGCCCA ATCCACTCGC CAACTCCAAT ACTAGTGGAT ACAGCGAAAG TAAGAAGGAC 5461 GACGCACGGG CTCAACTCAT GAAGGAGGAC CCGGAGCTTG CAAAGAGTTT CATCAAGACT 5521 CTCTTCGGCG TACTCTACGA GGTGTATTCT TCCAGTGCCG GTCCAGCTGT CAGGCACAAA 5581 TGTCTCAGGG CCATTCTCCG AATTATCTAT TTCGCCGATG CTGAGCTGCT CAAAGACGTC 5641 CTGAAGAACC ATGCCGTGTC TAGCCACATC GCTTCAATGC TGTCAAGCCA GGACCTGAAA 5701 ATCGTGGTTG GCGCCCTGCA GATGGCAGAG ATCTTGATGC AGAAGCTGCC CGATATCTTC 5761 TCCGTCTACT TCCGCAGAGA AGGGGTGATG CACCAGGTGA AACACCTGGC CGAGTCTGAA 5821 TCTCTGCTGA CATCTCCCCC AAAGGCATGT ACCAATGGAA GCGGATCCAT GGGTTCAACC 5881 ACATCTGTGT CCAGTGGTAC CGCCACCGCA GCCACACATG CTGCAGCAGA TCTCGGGAGC 5941 CCATCTCTTC AGCACTCAAG AGATGACAGT CTTGATCTGA GCCCGCAGGG CAGGCTGTCA 6001 GATGTGCTCA AACGGAAGCG CCTGCCTAAG AGGGGTCCAA GAAGGCCAAA GTACTCACCT 6061 CCAAGAGATG ATGACAAAGT AGACAATCAA GCTAAAAGCC CCACCACTAC TCAGTCACCT 6121 AAATCTTCTT TCCTGGCAAG CTTGAATCCA AAAACATGGG GAAGGTTAAG TACACAGTCC 6181 AACAGCAACA ACATTGAGCC AGCACGGACT GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC 6241 TCAAAGGATA CCATCTCCAA TAATAGAGAA AAAATTAAAG GTTGGATTAA GGAGCAGGCA 6301 CATAAATTTG TAGAACGTTA TTTCAGTTCT GAGAATATGG ATGGAAGCAA CCCTGCATTG 6361 AATGTCCTTC AGAGACTTTG TGCTGCAACC GAACAACTCA ACCTCCAGGT GGATGGTGGA 6421 GCTGAGTGCC TTGTAGAAAT CCGTAGCATA GTCTCAGAGT CAGATGTTTC ATCATTTGAA 6481 ATCCAACATA GTGGATTTGT GAAGCAGCTG TTGCTTTATT TGACATCTAA AAGTGAAAAG 6541 GATGCTGTGA GCAGAGAGAT CAGATTAAAG CGATTTCTTC ATGTATTTTT TTCTTCTCCA 6601 CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG GAACCAGTGG GTAATGCACC TTTGTTGGCA 6661 TTAGTTCACA AGATGAACAA CTGCCTCAGC CAGATGGAAC AATTTCCAGT CAAAGTACAT 6721 GATTTCCCTA GTGGAAATGG GACAGGAGGC AGCTTTTCTC TCAACAGAGG ATCACAGGCT 6781 TTAAAATTTT TCAACACACA TCAATTAAAA TGCCAGTTAC AAAGGCATCC AGACTGTGCA 6841 AATGTGAAGC AGTGGAAGGG TGGACCTGTC AAGATTGACC CTCTGGCTTT GGTACAAGCC 6901 ATCGAGAGAT ACCTTGTAGT TAGAGGGTAT GGAAGAGTAA GAGAAGATGA TGAAGACAGC 6961 GATGACGATG GATCAGATGA GGAAATAGAT GAGTCTCTGG CTGCTCAGTT CCTAAATTCA 7021 GGAAATGTAA GACACAGGCT GCAGTTTTAT ATTGGAGAAC ATTTGCTGCC GTATAACATG 7081 ACTGTGTATC AGGCAGTACG GCAGTTTAGT ATACAGGCTG AAGATGAAAG AGAATCCACA 7141 GATGATGAGA GCAATCCTCT AGGCAGAGCT GGTATTTGGA CAAAGACTCA TACAATATGG 7201 TATAAACCTG TGAGAGAGGA TGAAGAAAGT AATAAAGATT GTGTTGGTGG TAAAAGAGGA 7261 AGAGCCCAAA CAGCTCCAAC GAAAACTTCC CCTAGAAATG CAAAAAAGCA TGATGAGTTA 7321 TGGCACGATG GAGTGTGCCC ATCAGTATCA AATCCTTTAG AAGTTTACCT CATTCCCACA 7381 CCACCTGAAA ATATAACATT TGAAGACCCG TCATTAGATG TGATCCTTCT TTTAAGAGTT 7441 TTACATGCTA TCAGTCGATA CTGGTATTAC TTGTATGATA ATGCAATGTG CAAGGAAATT 7501 ATTCCAACTA GTGAATTTAT TAACAGTAAG TTAACAGCAA AAGCAAATAG GCAACTTCAA 7561 GATCCTTTAG TAATCATGAC AGGAAACATC CCAACATGGC TTACTGAGCT AGGAAAAACC 7621 TGCCCATTTT TCTTTCCTTT TGATACCCGG CAAATGCTTT TTTATGTAAC TGCATTTGAT 7681 CGGGACCGAG CAATGCAAAG ATTACTTGAT ACCAACCCAG AAATCAACCA GTCTGATTCT 7741 CAAGATAGCA GAGTTGCACC TAGATTGGAT AGAAAAAAAC GTACTGTGAA CCGAGAGGAG 7801 CTGCTGAAAC AGGCGGAGTC TGTGATGCAG GACCTCGGCA GCTCACGGGC CATGTTAGAA 7861 ATCCAGTATG AAAATGAGGT TGGTACAGGT CTTGGGCCTA CACTGGAGTT TTATGCGCTT 7921 GTATCTCAGG AACTACAGAG AGCTGACTTG GGTCTTTGGA GAGGTGAAGA AGTAACTCTT 7981 AGCAATCCAA AAGGGAGCCA AGAAGGGACC AAGTATATTC AAAACCTCCA GGGCCTGTTT 8041 GCGCTTCCCT TTGGTAGGAC AGCAAAGCCA GCTCATATCG CAAAGGTTAA GATGAAGTTT 8101 CGCTTCTTAG GAAAATTAAT GGCCAAGGCT ATCATGGATT TCAGATTGGT GGACCTTCCC 8161 CTTGGCTTAC CCTTTTATAA ATGGATGCTA CGGCAAGAAA CTTCACTGAC ATCACACGAT 8221 TTGTTTGACA TCGACCCAGT TGTAGCCAGA TCAGTTTATC ACCTAGAAGA CATTGTCAGA 8281 CAGAAGAAAA GACTTGAACA AGATAAATCC CAGACCAAAG AGAGTCTACA GTATGCATTA 8341 GAAACCTTGA CTATGAATGG CTGCTCAGTT GAAGATCTAG GACTGGATTT CACTCTGCCA 8401 GGGTTTCCCA ATATCGAACT GAAGAAAGGA GGGAAGGATA TACCAGTCAC TATCCACAAT 8461 TTAGAGGAGT ATCTAAGACT GGTTATATTC TGGGCACTAA ATGAAGGCGT TTCTAGGCAA 8521 TTTGATTCGT TCAGAGATGG ATTTGAATCA GTCTTCCCAC TCAGTCATCT TCAGTACTTC 8581 TACCCGGAGG AACTGGATCA GCTCCTTTGT GGCAGTAAAG CAGACACTTG GGATGCAAAG 8641 ACACTGATGG AATGCTGTAG GCCTGATCAT GGTTATACTC ATGACAGTCG GGCTGTGAAG 8701 TTTTTGTTTG AGATTCTCAG TAGTTTTGAT AATGAGCAGC AGAGGTTATT TCTCCAGTTT 8761 GTGACTGGTA GCCCAAGATT GCCTGTTGGA GGATTCCGGA GTTTGAATCC ACCTTTGACA 8821 ATTGTCCGAA AGACGTTTGA ATCAACAGAA AACCCAGATG ACTTCTTGCC CTCTGTAATG 8881 ACTTGTGTGA ACTATCTTAA GTTGCCGGAC TATTCAAGCA TTGAGATAAT GCGTGAAAAA 8941 CTGTTGATAG CAGCAAGAGA AGGGCAGCAG TCGTTCCATC TTTCCtaggc ggccgcggat 9001 cccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc 9061 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg 9121 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca 9181 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa 9241 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt 9301 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt 9361 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca 9421 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc 9481 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc 9541 acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccacaaggag 9601 acgaccttcc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga 9661 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt 9721 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt 9781 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt 9841 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg 9901 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga 9961 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg 10021 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc 10081 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc 10141 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc 10201 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg 10261 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc 10321 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc 10381 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag 10441 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt 10501 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 10561 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 10621 gagggcaaag gaatagacgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa 10681 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 10741 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 10801 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 10861 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 10921 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 10981 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 11041 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 11101 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 11161 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 11221 tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc 11281 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga 11341 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa 11401 ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca 11461 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc 11521 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg 11581 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac 11641 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga 11701 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta 11761 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg 11821 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc 11881 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 11941 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 12001 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 12061 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 12121 caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgttta ccgtcgacct 12181 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 12241 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 12301 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 12361 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 12421 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 12481 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 12541 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 12601 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 12661 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 12721 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 12781 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 12841 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 12901 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 12961 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 13021 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 13081 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 13141 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 13201 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 13261 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 13321 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 13381 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 13441 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 13501 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 13561 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 13621 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 13681 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 13741 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 13801 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 13861 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 13921 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 13981 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 14041 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 14101 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 14161 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 14221 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 14281 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 14341 cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat 14401 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 14461 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg 14521 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc 14581 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa 14641 agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt 14701 gttaaatatg tactacaaac ttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???