pLV2-CMV-NTMT1-HA-Puro plasmid

PVT105847

pLV2-CMV-NTMT1-HA-Puro plasmid Information

Plasmid name: pLV2-CMV-NTMT1(human)-HA-Puro
Alias: NM_001286796.2;AD-003; C9orf32; HOMT1A; METTL11A; NRMT; NRMT1; NTM1A
Gene lenght: 669
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-NTMT1-HA-Puro plasmid Description

pLV2-CMV-NTMT1-HA-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV2-CMV-NTMT1-HA-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgacgag cgaggtgata gaagacgaga agcaattcta 2881 ttccaaggcc aagacctact ggaaacaaat cccacccacg gtggacggca tgcttggggg 2941 gtatggccac atctccagca tcgacatcaa cagctcccgg aagtttctgc agaggttttt 3001 gagggaaggc ccgaacaaga caggaacgtc ctgtgccctg gactgtggag ctggcattgg 3061 gaggatcacc aagcggctgc tcctgccgct gttcagagag gtggatatgg tcgacataac 3121 ggaggacttc ctggttcaag ccaagaccta cctgggggag gagggcaaga gggtgaggaa 3181 ctacttctgt tgtgggctcc aggacttcac cccggagccg gactcttacg acgtgatctg 3241 gatccagtgg gtgataggcc acctcaccga tcagcacctg gccgagttcc tgcggcgctg 3301 caagggcagc ctccgcccca acggcatcat cgtcatcaaa gacaacatgg cccaggaggg 3361 cgtgattctg gacgacgtgg acagcagcgt gtgccgggac cttgacgtgg tccgcaggat 3421 catctgcagt gcaggcctca gcctcctggc cgaggagagg caggagaacc tccccgatga 3481 gatctaccat gtctatagct ttgccctgag aggatccTAC CCATACGACG TACCAGATTA 3541 CGCTtagtct agataattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc 3601 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac 3661 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc 3721 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag 3781 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga 3841 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt 3901 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg 3961 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac 4021 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt 4081 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 4141 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 4201 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 4261 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 4321 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 4381 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 4441 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 4501 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 4561 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 4621 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 4681 tgaccgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 4741 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 4801 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 4861 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 4921 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 4981 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 5041 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc 5101 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 5161 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 5221 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc 5281 cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc 5341 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg 5401 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat 5461 cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga 5521 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac 5581 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta 5641 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac 5701 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac 5761 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc 5821 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg 5881 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg 5941 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 6001 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 6061 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 6121 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 6181 atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct agctagagct 6241 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 6301 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 6361 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 6421 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 6481 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 6541 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 6601 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 6661 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 6721 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 6781 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 6841 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 6901 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 6961 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 7021 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 7081 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 7141 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 7201 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 7261 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 7321 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 7381 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 7441 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 7501 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 7561 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 7621 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 7681 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 7741 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 7801 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 7861 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 7921 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 7981 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 8041 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 8101 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 8161 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 8221 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 8281 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 8341 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 8401 cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa 8461 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 8521 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct 8581 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg 8641 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt 8701 tgttaaatat gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt taaatatgta 8761 ctacaaactt agtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???