pLV2-CMV-RBM24-HA-Puro plasmid

PVT104946

pLV2-CMV-RBM24-HA-Puro plasmid Information

Plasmid name: pLV2-CMV-RBM24(human)-HA-Puro
Alias: NM_001143942.2;dJ259A10.1; RNPC6;
Gene lenght: 708
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-RBM24-HA-Puro plasmid Description

pLV2-CMV-RBM24-HA-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-RBM24-HA-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgcacac gacccagaag gacacgacgt acaccaagat 2881 cttcgtcggg gggctgccct accacaccac cgacgccagc ctgcgcaagt acttcgaggt 2941 cttcggcgag atcgaggagg cggtggtcat caccgaccgg cagacgggca agtcccgggg 3001 ctatggattt gtcaccatgg ctgaccgggc tgctgccgaa agggcctgca aggatcccaa 3061 tcccatcatt gatggcagaa aggccaacgt gaacctggca tacttaggag caaaaccaag 3121 gatcatgcaa ccaggttttg cctttggtgt tcaacaactt catccagccc ttatacaaag 3181 acctttcggg atacctgccc actatgtcta tccgcaggct tttgtgcagc cgggagtggt 3241 cattccacac gtccagccga cagcagctgc cgcctccacc accccttaca ttgattacac 3301 tggagctgca tacgcacaat actcagcagc tgctgctgct gccgccgccg ctgctgccta 3361 tgaccagtac ccctatgcag cctctccagc tgctgcagga tatgttactg ctgggggcta 3421 tggctacgca gtccagcagc caatcaccgc agcggcacct gggacagctg ccgccgccgc 3481 tgcagcagct gctgccgctg cagcatttgg ccagtaccag cctcagcagc tgcagacaga 3541 ccgaatgcaa ggatccTACC CATACGACGT ACCAGATTAC GCTtagtcta gataattcta 3601 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct 3661 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag 3721 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt 3781 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc 3841 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct 3901 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag 3961 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc 4021 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc 4081 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc 4141 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 4201 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 4261 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 4321 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 4381 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 4441 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 4501 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 4561 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 4621 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 4681 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct ggaacaatca 4741 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 4801 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 4861 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 4921 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 4981 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 5041 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 5101 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 5161 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 5221 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 5281 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc 5341 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct 5401 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag 5461 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 5521 aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg 5581 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc 5641 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta 5701 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg 5761 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac 5821 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag 5881 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga 5941 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga 6001 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 6061 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 6121 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 6181 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg 6241 acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 6301 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6361 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6421 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6481 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6541 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6601 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6661 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6721 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6781 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6841 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6901 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 6961 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7021 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7081 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 7141 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7201 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7261 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7321 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7381 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7441 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7501 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7561 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7621 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7681 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7741 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7801 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7861 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7921 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 7981 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8041 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8101 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8161 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8221 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 8281 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8341 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8401 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 8461 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 8521 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 8581 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt 8641 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt 8701 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac 8761 ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???