pLV2-CMV-Sting1-Puro plasmid

PVT91478

pLV2-CMV-Sting1-Puro plasmid Information

Plasmid name: pLV2-CMV-Sting1(mouse)-Puro
Alias: NM_028261.1;2610307O08Rik; ERIS; Mita; MPYS; STING;STING-beta; Tmem173
Gene lenght: 1137bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-Sting1-Puro plasmid Description

pLV2-CMV-Sting1-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-Sting1-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgccata ctccaacctg catccagcca tcccacggcc 2881 cagaggtcac cgctccaaat atgtagccct catctttctg gtggccagcc tgatgatcct 2941 ttgggtggca aaggatccac caaatcacac tctgaagtac ctagcacttc acctagcctc 3001 gcacgaactt ggactactgt tgaaaaacct ctgctgtctg gctgaagagc tgtgccatgt 3061 ccagtccagg taccagggca gctactggaa ggctgtgcgc gcctgcctgg gatgccccat 3121 ccactgtatg gctatgattc tactatcgtc ttatttctat ttcctccaaa acactgctga 3181 catatacctc agttggatgt ttggccttct ggtcctctat aagtccctaa gcatgctcct 3241 gggccttcag agcttgactc cagcggaagt ctctgcagtc tgtgaagaaa agaagttaaa 3301 tgttgcccac gggctggcct ggtcatacta cattgggtac ttgcggttga tcttaccagg 3361 gctccaggcc cggatccgaa tgttcaatca gctacataac aacatgctca gtggtgcagg 3421 gagccgaaga ctgtacatcc tctttccatt ggactgtggg gtgcctgaca acctgagtgt 3481 agttgacccc aacattcgat tccgagatat gctgccccag caaaacatcg accgtgctgg 3541 catcaagaat cgggtttatt ccaacagcgt ctacgagatt ctggagaacg gacagccagc 3601 aggcgtctgt atcctggagt acgccacccc cttgcagacc ctgtttgcca tgtcacagga 3661 tgccaaagct ggcttcagtc gggaggatcg gcttgagcag gctaaactct tctgccggac 3721 acttgaggaa atcctggaag atgtccccga gtctcgaaat aactgccgcc tcattgtcta 3781 ccaagaaccc acagacggaa acagtttctc actgtctcag gaggtgctcc ggcacattcg 3841 tcaggaagaa aaggaggagg ttaccatgaa tgcccccatg acctcagtgg cacctcctcc 3901 ctccgtactg tcccaagagc caagactcct catcagtggt atggatcagc ctctcccact 3961 ccgcactgac ctcatctgag gatcccgcga ctctagataa ttctaccggg taggggaggc 4021 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta 4081 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc 4141 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc 4201 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct 4261 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca 4321 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg 4381 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc 4441 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg 4501 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc 4561 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 4621 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 4681 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 4741 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 4801 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 4861 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 4921 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 4981 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 5041 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 5101 tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa 5161 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 5221 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 5281 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 5341 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 5401 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 5461 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 5521 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 5581 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 5641 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 5701 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa 5761 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta 5821 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca 5881 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa 5941 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa 6001 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc 6061 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa 6121 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga 6181 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg 6241 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg 6301 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc 6361 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg 6421 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 6481 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 6541 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 6601 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt 6661 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 6721 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 6781 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 6841 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 6901 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 6961 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 7021 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 7081 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 7141 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 7201 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 7261 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 7321 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 7381 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 7441 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 7501 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 7561 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 7621 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 7681 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 7741 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 7801 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 7861 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 7921 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 7981 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 8041 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 8101 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 8161 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 8221 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 8281 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 8341 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 8401 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 8461 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 8521 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 8581 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 8641 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 8701 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 8761 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 8821 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt 8881 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 8941 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 9001 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct 9061 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta 9121 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa 9181 gaaattgtat ttgttaaata tgtactacaa acttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???