pLV2-CMV-TOMM20-mTagBFP-Puro plasmid

PVT107518

pLV2-CMV-TOMM20-mTagBFP-Puro plasmid Information

Plasmid name: pLV2-CMV-TOMM20(human)-mTagBFP-Puro
Alias: NM_014765.3;MAS20; MOM19; TOM20
Gene lenght: 435
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Blue
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-TOMM20-mTagBFP-Puro plasmid Description

pLV2-CMV-TOMM20-mTagBFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV2-CMV-TOMM20-mTagBFP-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ccaccatggt gggtcggaac agcgccatcg ccgccggtgt atgcggggcc cttttcattg 2881 ggtactgcat ctacttcgac cgcaaaagac gaagtgaccc caacttcaag aacaggcttc 2941 gagaacgaag aaagaaacag aagcttgcca aggagagagc tgggctttcc aagttacctg 3001 accttaaaga tgctgaagct gttcagaagt tcttccttga agaaatacag cttggtgaag 3061 agttactagc tcaaggtgaa tatgagaagg gcgtagacca tctgacaaat gcaattgctg 3121 tgtgtggaca gccacagcag ttactgcagg tcttacagca aactcttcca ccaccagtgt 3181 tccagatgct tctgactaag ctcccaacaa ttagtcagag aattgtaagt gctcagagct 3241 tggctgaaga tgatgtggaa ggaTCCGGCG GTGGAGGAAG Cggcggaggt ggatctggaa 3301 ttcacggtgc ccgggccacc atggtgtcta agggcgaaga gctgattaag gagaacatgc 3361 acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg 3421 gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc 3481 ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc aagaccttca 3541 tcaaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag ggcttcacat 3601 gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc 3661 tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc acatccaacg 3721 gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgagacg ctgtaccccg 3781 ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc 3841 tgatcgcaaa cgccaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc 3901 ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgagacct 3961 acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc aaactggggc 4021 acaagcttaa ttaatctaga taattctacc gggtagggga ggcgcttttc ccaaggcagt 4081 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc 4141 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct 4201 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt 4261 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca 4321 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct 4381 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc 4441 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa 4501 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc 4561 caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 4621 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 4681 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 4741 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 4801 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 4861 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 4921 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 4981 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 5041 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 5101 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc 5161 cggtgcctga ccgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga 5221 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 5281 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 5341 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 5401 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 5461 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 5521 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 5581 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 5641 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 5701 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 5761 ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac 5821 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga 5881 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc 5941 tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca 6001 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta 6061 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa 6121 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt 6181 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag 6241 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa 6301 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg 6361 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt 6421 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 6481 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 6541 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 6601 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 6661 aatgaatatc agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc 6721 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 6781 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 6841 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 6901 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 6961 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 7021 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 7081 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 7141 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 7201 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 7261 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 7321 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 7381 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 7441 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 7501 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 7561 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 7621 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 7681 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 7741 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 7801 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 7861 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 7921 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 7981 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 8041 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 8101 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 8161 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 8221 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 8281 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 8341 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 8401 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 8461 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 8521 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 8581 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 8641 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 8701 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 8761 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 8821 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 8881 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg 8941 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 9001 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac 9061 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta 9121 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa 9181 ttgtatttgt taaatatgta ctacaaactt agtagttttt aaagaaattg tatttgttaa 9241 atatgtacta caaacttagt agt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.