pLV2-CMV-TXNRD1-mCherry-SECIS-Puro plasmid

PVT95939

pLV2-CMV-TXNRD1-mCherry-SECIS-Puro plasmid Information

Plasmid name: pLV2-CMV-TXNRD1(human)-mCherry-SECIS-Puro
Alias: NM_182729.3;GRIM-12; TR; TR1; TRXR1; TXNR; TXNR1
Gene lenght: 1497bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-TXNRD1-mCherry-SECIS-Puro plasmid Description

pLV2-CMV-TXNRD1-mCherry-SECIS-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-TXNRD1-mCherry-SECIS-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgaacgg ccctgaagat cttcccaagt cctatgacta 2881 tgaccttatc atcattggag gtggctcagg aggtctggca gctgctaagg aggcagccca 2941 atatggcaag aaggtgatgg tcctggactt tgtcactccc acccctcttg gaactagatg 3001 gggtctcgga ggaacatgtg tgaatgtggg ttgcatacct aaaaaactga tgcatcaagc 3061 agctttgtta ggacaagccc tgcaagactc tcgaaattat ggatggaaag tcgaggagac 3121 agttaagcat gattgggaca gaatgataga agctgtacag aatcacattg gctctttgaa 3181 ttggggctac cgagtagctc tgcgggagaa aaaagtcgtc tatgagaatg cttatgggca 3241 atttattggt cctcacagga ttaaggcaac aaataataaa ggcaaagaaa aaatttattc 3301 agcagagaga tttctcattg ccactggtga aagaccacgt tacttgggca tccctggtga 3361 caaagaatac tgcatcagca gtgatgatct tttctccttg ccttactgcc cgggtaagac 3421 cctggttgtt ggagcatcct atgtcgcttt ggagtgcgct ggatttcttg ctggtattgg 3481 tttagacgtc actgttatgg ttaggtccat tcttcttaga ggatttgacc aggacatggc 3541 caacaaaatt ggtgaacaca tggaagaaca tggcatcaag tttataagac agttcgtacc 3601 aattaaagtt gaacaaattg aagcagggac accaggccga ctcagagtag tagctcagtc 3661 caccaatagt gaggaaatca ttgaaggaga atataatacg gtgatgctgg caataggaag 3721 agatgcttgc acaagaaaaa ttggcttaga aaccgtaggg gtgaagataa atgaaaagac 3781 tggaaaaata cctgtcacag atgaagaaca gaccaatgtg ccttacatct atgccattgg 3841 cgatatattg gaggataagg tggagctcac cccagttgca atccaggcag gaagattgct 3901 ggctcagagg ctctatgcag gttccactgt caagtgtgac tatgaaaatg ttccaaccac 3961 tgtatttact cctttggaat atggtgcttg tggcctttct gaggagaaag ctgtggagaa 4021 gtttggggaa gaaaatattg aggtttacca tagttacttt tggccattgg aatggacgat 4081 tccgtcaaga gataacaaca aatgttatgc aaaaataatc tgtaatacta aagacaatga 4141 acgtgttgtg ggctttcacg tactgggtcc aaatgctgga gaagttacac aaggctttgc 4201 agctgcgctc aaatgtggac tgaccaaaaa gcagctggac agcacaattg gaatccaccc 4261 tgtctgtgca gaggtattca caacattgtc tgtgaccaag cgctctgggg caagcatcct 4321 ccaggctggc tgctgaggtg gtggaggcgg atcaggaggc ggtggatcta tggtgagcaa 4381 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga 4441 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga 4501 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga 4561 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat 4621 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt 4681 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat 4741 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa 4801 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg 4861 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac 4921 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt 4981 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg 5041 ccgccactcc accggcggca tggacgagct gtacaagtag catttggcag ggcatcgaag 5101 ggatgcatcc atgaagtcac cagtctcaag cccatgtggt aggcggtgat ggaacaactg 5161 tcaaatcgga tcccgcgact ctagataatt ctaccgggta ggggaggcgc ttttcccaag 5221 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct 5281 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg 5341 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct 5401 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga 5461 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg 5521 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg 5581 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct 5641 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg 5701 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 5761 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 5821 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 5881 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 5941 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 6001 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 6061 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 6121 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 6181 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 6241 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 6301 aagcccggtg cctgaccgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag 6361 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 6421 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 6481 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 6541 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 6601 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 6661 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 6721 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 6781 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 6841 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct 6901 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca 6961 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag 7021 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag 7081 gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac 7141 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct 7201 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc 7261 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc 7321 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag 7381 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac 7441 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg 7501 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct 7561 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 7621 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 7681 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 7741 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 7801 aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttta ccgtcgacct 7861 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 7921 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 7981 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 8041 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 8101 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 8161 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 8221 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 8281 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 8341 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 8401 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 8461 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 8521 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 8581 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 8641 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 8701 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 8761 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 8821 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 8881 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 8941 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 9001 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 9061 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 9121 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 9181 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 9241 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 9301 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 9361 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 9421 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 9481 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 9541 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 9601 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 9661 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 9721 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 9781 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 9841 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 9901 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 9961 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 10021 cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat 10081 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 10141 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg 10201 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc 10261 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa 10321 agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt 10381 gttaaatatg tactacaaac ttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???