pLV2-CMV-ZBTB9-3×FLAG-PGK-Puro plasmid

PVT108445

pLV2-CMV-ZBTB9-3×FLAG-PGK-Puro plasmid Information

Plasmid name: pLV2-CMV-ZBTB9(human)-3×FLAG-PGK-Puro
Alias: NM_152735.4;ZNF919
Gene lenght: 1419
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-ZBTB9-3×FLAG-PGK-Puro plasmid Description

pLV2-CMV-ZBTB9-3×FLAG-PGK-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-ZBTB9-3×FLAG-PGK-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggaaac cccaacacct ttgccgcctg tacccgcctc 2881 cccgacctgc aacccagccc cacggacaat ccagatcgag ttcccacagc atagctcgtc 2941 tctgctggaa tctctgaacc gccacaggct agagggaaag ttctgtgatg tgtccctcct 3001 ggtgcagggc cgggaactta gggctcataa agcagtgtta gctgctgcct ctccttactt 3061 ccatgacaag ctgcttctgg gggatgcgcc tcgtctcact ctaccgagtg tcattgaagc 3121 cgatgccttc gaggggctgc tccagctcat ttattcaggg cgtctccgcc tgccactgga 3181 tgctcttcct gctcatctcc ttgtggccag tggccttcaa atgtggcagg tagtagatca 3241 gtgctcagaa attcttagag aattagaaac ttcaggtggt ggaatttcag cccgtggagg 3301 aaactcctac catgcccttc tttccactac atcctctaca ggaggctggt gcattcgctc 3361 ttcgcctttc cagaccccag tacagtcctc tgcttctact gaaagccctg cttccactga 3421 gagccctgtg ggaggggagg gaagtgaact gggagaagtg ctgcaaattc aggtggaaga 3481 agaagaggag gaggaggaag atgatgatga tgaggaccag gggtcagcca cactctctca 3541 gactcctcag ccccagagag tatcaggggt ttttccccgt cctcatggac cccacccact 3601 gcccatgact gctactcccc gaaagcttcc agagggtgag agtgcaccac ttgagcttcc 3661 tgcccctcct gcactgcccc ccaaaatctt ctacattaag caggaaccct tcgagcctaa 3721 ggaggagata tcaggaagcg gaactcagcc tggaggagca aaggaggaaa ccaaagtgtt 3781 ttctggaggg gacactgaag ggaatgggga gctagggttc ttgttgcctt cagggccagg 3841 gccaacatct gggggagggg gtccatcctg gaaaccagtg gatcttcatg ggaatgaaat 3901 cctgtcaggg ggtggaggac ctgggggagc aggccaggcc gtgcatgggc ctgtgaagct 3961 aggggggaca ccccctgcag atggaaaacg ctttggttgc ctgtgtggga agcggtttgc 4021 agtgaagcca aagcgtgacc ggcacatcat gctgaccttc agccttcggc cttttggctg 4081 tggcatctgc aacaagcgct tcaagctgaa gcaccatctg acagagcaca tgaagaccca 4141 tgctggagcc ctgcatgcct gtccccactg tggccgtcgg ttccgagtcc atgcctgttt 4201 tctccgccac cgggacctat gcaagggcca gggctgggcc actgcccact ggacttacaa 4261 gggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA 4321 TGACGATGAC AAGTAAtcta gataattcta ccgggtaggg gaggcgcttt tcccaaggca 4381 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg 4441 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc 4501 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc 4561 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag 4621 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc 4681 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg 4741 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca 4801 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag 4861 cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 4921 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 4981 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 5041 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 5101 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 5161 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 5221 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 5281 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 5341 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 5401 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 5461 cccggtgcct gaccgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt 5521 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 5581 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 5641 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 5701 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 5761 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 5821 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 5881 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 5941 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 6001 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg 6061 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc 6121 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag 6181 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca 6241 gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc 6301 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat 6361 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac 6421 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc 6481 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg 6541 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc 6601 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg 6661 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct 6721 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 6781 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 6841 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 6901 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 6961 gaaatgaata tcagagagtg agaggccttg acattgctag cgttttaccg tcgacctcta 7021 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 7081 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 7141 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 7201 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 7261 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 7321 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 7381 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 7441 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 7501 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 7561 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 7621 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 7681 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 7741 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 7801 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 7861 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 7921 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 7981 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 8041 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 8101 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 8161 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 8221 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 8281 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 8341 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 8401 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 8461 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 8521 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 8581 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 8641 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 8701 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 8761 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 8821 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 8881 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 8941 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 9001 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 9061 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 9121 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 9181 gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat 9241 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 9301 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata 9361 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat 9421 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga 9481 aattgtattt gttaaatatg tactacaaac ttagtagttt ttaaagaaat tgtatttgtt 9541 aaatatgtac tacaaactta gtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.