pLV2-EF1α-CD24-IRES-ZsGreen1-PGK-Puro plasmid

PVT99098

pLV2-EF1α-CD24-IRES-ZsGreen1-PGK-Puro plasmid Information

Plasmid name: pLV2-EF1α-CD24(human)-IRES-ZsGreen1-PGK-Puro
Alias: NM_013230.3;CD24A;
Gene lenght: 240bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: EF1α
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pLV2-EF1α-CD24-IRES-ZsGreen1-PGK-Puro plasmid Description

pLV2-EF1α-CD24-IRES-ZsGreen1-PGK-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-EF1α-CD24-IRES-ZsGreen1-PGK-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagTAA TTCATACAAA AGGACTCGCC CCTGCCTTGG 2221 GGAATCCCAG GGACCGTCGT TAAACTCCCA CTAACGTAGA ACCCAGAGAT CGCTGCGTTC 2281 CCGCCCCCTC ACCCGCCCGC TCTCGTCATC ACTGAGGTGG AGAAGAGCAT GCGTGAGGCT 2341 CCGGTGCCCG TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG 2401 GGGTCGGCAA TTGAACCGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG 2461 TCGTGTACTG GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG 2521 TCGCCGTGAA CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGGTA AGTGCCGTGT 2581 GTGGTTCCCG CGGGCCTGGC CTCTTTACGG GTTATGGCCC TTGCGTGCCT TGAATTACTT 2641 CCACGCCCCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG 2701 GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG 2761 CTTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT 2821 TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG 2881 CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC 2941 GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA 3001 GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT 3061 GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA 3121 GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG 3181 ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG 3241 TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT 3301 TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG 3361 GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA 3421 TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA 3481 GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAA CCGGTGCGGC CGCATAGGAT 3541 CCGCCACCat gggcagagca atggtggcca ggctcgggct ggggctgctg ctgctggcac 3601 tgctcctacc cacgcagatt tattccagtg aaacaacaac tggaacttca agtaactcct 3661 cccagagtac ttccaactct gggttggccc caaatccaac taatgccacc accaaggcgg 3721 ctggtggtgc cctgcagtca acagccagtc tcttcgtggt ctcactctct cttctgcatc 3781 tctactctta AGATCTAGAT TCACCGGTCG CCCCTCTCCC TCCCCCCCCC CTAACGTTAC 3841 TGGCCGAAGC CGCTTGGAAT AAGGCCGGTG TGCGTTTGTC TATATGTTAT TTTCCACCAT 3901 ATTGCCGTCT TTTGGCAATG TGAGGGCCCG GAAACCTGGC CCTGTCTTCT TGACGAGCAT 3961 TCCTAGGGGT CTTTCCCCTC TCGCCAAAGG AATGCAAGGT CTGTTGAATG TCGTGAAGGA 4021 AGCAGTTCCT CTGGAAGCTT CTTGAAGACA AACAACGTCT GTAGCGACCC TTTGCAGGCA 4081 GCGGAACCCC CCACCTGGCG ACAGGTGCCT CTGCGGCCAA AAGCCACGTG TATAAGATAC 4141 ACCTGCAAAG GCGGCACAAC CCCAGTGCCA CGTTGTGAGT TGGATAGTTG TGGAAAGAGT 4201 CAAATGGCTC ACCTCAAGCG TATTCAACAA GGGGCTGAAG GATGCCCAGA AGGTACCCCA 4261 TTGTATGGGA TCTGATCTGG GGCCTCGGTG CACATGCTTT ACATGTGTTT AGTCGAGGTT 4321 AAAAAACGTC TAGGCCCCCC GAACCACGGG GACGTGGTTT TCCTTTGAAA AACACGATGA 4381 TAATACCGGT CGATCCACAA CCTGTACAGC CACCatggcc cagtccaagc acggcctgac 4441 caaggagatg accatgaagt accgcatgga gggctgcgtg gacggccaca agttcgtgat 4501 caccggcgag ggcatcggct accccttcaa gggcaagcag gccatcaacc tgtgcgtggt 4561 ggagggcggc cccttgccct tcgccgagga catcttgtcc gccgccttca tgtacggcaa 4621 ccgcgtgttc accgagtacc cccaggacat cgtcgactac ttcaagaact cctgccccgc 4681 cggctacacc tgggaccgct ccttcctgtt cgaggacggc gccgtgtgca tctgcaacgc 4741 cgacatcacc gtgagcgtgg aggagaactg catgtaccac gagtccaagt tctacggcgt 4801 gaacttcccc gccgacggcc ccgtgatgaa gaagatgacc gacaactggg agccctcctg 4861 cgagaagatc atccccgtgc ccaagcaggg catcttgaag ggcgacgtga gcatgtacct 4921 gctgctgaag gacggtggcc gcttgcgctg ccagttcgac accgtgtaca aggccaagtc 4981 cgtgccccgc aagatgcccg actggcactt catccagcac aagctgaccc gcgaggaccg 5041 cagcgacgcc aagaaccaga agtggcacct gaccgagcac gccatcgcct ccggctccgc 5101 cttgccctga GAATTCTAAT CTAGAACGCG ATtaattcta ccgggtaggg gaggcgcttt 5161 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa 5221 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct 5281 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc 5341 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc 5401 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc 5461 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc 5521 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct 5581 gcacgcttcg aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc 5641 gaATTCAAGG GCAGAGGAAG TCTTCTAACT TGAGGTGACG TGGAGGAGAA TCCCGGCCCT 5701 ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC CAGGGCCGTA 5761 CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT CGATCCGGAC 5821 CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC 5881 ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG 5941 AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT 6001 TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG 6061 CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC 6121 AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG 6181 GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC 6241 GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA 6301 ACGCGTCGAC ACAGTCAGAC ACAGTCCAGC GATCGCGACA GACGCAGTCG GCGCGCCGGA 6361 ACAATCAACC TCTGgattac aaaatttgtg aaagattgac tggtattctt aactatgttg 6421 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 6481 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 6541 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 6601 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 6661 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 6721 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 6781 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 6841 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 6901 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta 6961 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac 7021 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac 7081 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 7141 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga 7201 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc 7261 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga 7321 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca 7381 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt 7441 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg 7501 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga 7561 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct 7621 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 7681 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 7741 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 7801 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 7861 ggccttgaca ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg 7921 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 7981 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 8041 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 8101 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 8161 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 8221 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 8281 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 8341 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 8401 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 8461 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 8521 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 8581 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 8641 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 8701 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 8761 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 8821 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 8881 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 8941 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 9001 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 9061 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 9121 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 9181 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 9241 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 9301 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 9361 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 9421 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 9481 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 9541 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 9601 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 9661 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 9721 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 9781 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 9841 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 9901 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 9961 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 10021 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac 10081 ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 10141 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 10201 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc 10261 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta 10321 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac 10381 tacaaactta gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta 10441 gt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???