pLV2-EF1α-CD19-Puro plasmid

PVT99972

pLV2-EF1α-CD19-Puro plasmid Information

Plasmid name: pLV2-EF1α-CD19(human)-Puro
Alias: NM_001178098.2;B4; CVID3
Gene lenght: 1647bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: IRES-R
Copy number: High
Induction:

pLV2-EF1α-CD19-Puro plasmid Description

pLV2-EF1α-CD19-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-EF1α-CD19-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc 3541 GCCACCatgc cacctcctcg cctcctcttc ttcctcctct tcctcacccc catggaagtc 3601 aggcccgagg aacctctagt ggtgaaggtg gaagagggag ataacgctgt gctgcagtgc 3661 ctcaagggga cctcagatgg ccccactcag cagctgacct ggtctcggga gtccccgctt 3721 aaacccttct taaaactcag cctggggctg ccaggcctgg gaatccacat gaggcccctg 3781 gccatctggc ttttcatctt caacgtctct caacagatgg ggggcttcta cctgtgccag 3841 ccggggcccc cctctgagaa ggcctggcag cctggctgga cagtcaatgt ggagggcagc 3901 ggggagctgt tccggtggaa tgtttcggac ctaggtggcc tgggctgtgg cctgaagaac 3961 aggtcctcag agggccccag ctccccttcc gggaagctca tgagccccaa gctgtatgtg 4021 tgggccaaag accgccctga gatctgggag ggagagcctc cgtgtctccc accgagggac 4081 agcctgaacc agagcctcag ccaggacctc accatggccc ctggctccac actctggctg 4141 tcctgtgggg taccccctga ctctgtgtcc aggggccccc tctcctggac ccatgtgcac 4201 cccaaggggc ctaagtcatt gctgagccta gagctgaagg acgatcgccc ggccagagat 4261 atgtgggtaa tggagacggg tctgttgttg ccccgggcca cagctcaaga cgctggaaag 4321 tattattgtc accgtggcaa cctgaccatg tcattccacc tggagatcac tgctcggcca 4381 gtactatggc actggctgct gaggactggt ggctggaagg tctcagctgt gactttggct 4441 tatctgatct tctgcctgtg ttcccttgtg ggcattcttc atcttcaaag agccctggtc 4501 ctgaggagga aaagaaagcg aatgactgac cccaccagga gattcttcaa agtgacgcct 4561 cccccaggaa gcgggcccca gaaccagtac gggaacgtgc tgtctctccc cacacccacc 4621 tcaggcctcg gacgcgccca gcgttgggcc gcaggcctgg ggggcactgc cccgtcttat 4681 ggaaacccga gcagcgacgt ccaggcggat ggagccttgg ggtcccggag cccgccggga 4741 gtgggcccag aagaagagga aggggagggc tatgaggaac ctgacagtga ggaggactcc 4801 gagttctatg agaacgactc caaccttggg caggaccagc tctcccagga tggcagcggc 4861 tacgagaacc ctgaggatga gcccctgggt cctgaggatg aagactcctt ctccaacgct 4921 gagtcttatg agaacgagga tgaagagctg acccagccgg tcgccaggac aatggacttc 4981 ctgagccctc atgggtcagc ctgggacccc agccgggaag caacctccct ggcagggtcc 5041 cagtcctatg aggatatgag aggaatcctg tatgcagccc cccagctccg ctccattcgg 5101 ggccagcctg gacccaatca tgaggaagat gcagactctt atgagaacat ggataatccc 5161 gatgggccag acccagcctg gggaggaggg ggccgcatgg gcacctggag caccaggtga 5221 tctagagcgg ccgcggatcc cgcccctctc cctccccccc ccctaacgtt actggccgaa 5281 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 5341 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 5401 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 5461 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 5521 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 5581 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 5641 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 5701 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac 5761 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt 5821 gccacaaccc acaaggagac gaccttccat gaccgagtac aagcccacgg tgcgcctcgc 5881 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 5941 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga 6001 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 6061 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 6121 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 6181 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 6241 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 6301 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 6361 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 6421 gtgcatgacc cgcaagcccg gtgcctagac gcgtctggaa caatcaacct ctggattaca 6481 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 6541 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 6601 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 6661 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 6721 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 6781 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 6841 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 6901 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 6961 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 7021 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga 7081 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct 7141 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc 7201 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga 7261 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca 7321 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac 7381 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa 7441 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag 7501 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct 7561 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg 7621 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg 7681 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct 7741 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 7801 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 7861 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 7921 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt 7981 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 8041 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 8101 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 8161 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 8221 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 8281 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 8341 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 8401 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 8461 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 8521 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 8581 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 8641 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 8701 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 8761 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 8821 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 8881 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 8941 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 9001 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 9061 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 9121 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 9181 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 9241 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 9301 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 9361 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 9421 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 9481 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 9541 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 9601 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 9661 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 9721 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 9781 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 9841 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 9901 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 9961 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 10021 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 10081 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 10141 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt 10201 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 10261 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 10321 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct 10381 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta 10441 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agt

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???