pLV2-EF1a-strep Ⅱ-6×His-BDKRB2-3×FLAG-IRES-Puro plasmid

PVT108036

pLV2-EF1a-strep Ⅱ-6×His-BDKRB2-3×FLAG-IRES-Puro plasmid Information

Plasmid name: pLV2-EF1a-strep Ⅱ-6×His-BDKRB2(human)-3×FLAG-IRES-Puro
Alias:
Gene lenght: 1173
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF-1α promoter-F1
Reverse primer:
Copy number: High
Induction:

pLV2-EF1a-strep Ⅱ-6×His-BDKRB2-3×FLAG-IRES-Puro plasmid Description

pLV2-EF1a-strep Ⅱ-6×His-BDKRB2-3×FLAG-IRES-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-EF1a-strep Ⅱ-6×His-BDKRB2-3×FLAG-IRES-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaATTAGCTT GGTACgaatt 3541 cGCCACCATG TGGTCCCACC CCCAGTTTGA GAAGGGCGGC GGCAGCGGCG GCGGAAGCGG 3601 AGGAAGCGCT TGGTCCCATC CCCAGTTCGA GAAGCACCAT CACCATCACC ATatgttctc 3661 tccctggaag atatcaatgt ttctgtctgt tcgtgaggac tccgtgccca ccacggcctc 3721 tttcagcgcc gacatgctca atgtcacctt gcaagggccc actcttaacg ggacctttgc 3781 ccagagcaaa tgcccccaag tggagtggct gggctggctc aacaccatcc agcccccctt 3841 cctctgggtg ctgttcgtgc tggccaccct agagaacatc tttgtcctca gcgtcttctg 3901 cctgcacaag agcagctgca cggtggcaga gatctacctg gggaacctgg ccgcagcaga 3961 cctgatcctg gcctgcgggc tgcccttctg ggccatcacc atctccaaca acttcgactg 4021 gctctttggg gagacgctct gccgcgtggt gaatgccatt atctccatga acctgtacag 4081 cagcatctgt ttcctgatgc tggtgagcat cgaccgctac ctggccctgg tgaaaaccat 4141 gtccatgggc cggatgcgcg gcgtgcgctg ggccaagctc tacagcttgg tgatctgggg 4201 gtgtacgctg ctcctgagct cacccatgct ggtgttccgg accatgaagg agtacagcga 4261 tgagggccac aacgtcaccg cttgtgtcat cagctaccca tccctcatct gggaagtgtt 4321 caccaacatg ctcctgaatg tcgtgggctt cctgctgccc ctgagtgtca tcaccttctg 4381 cacgatgcag atcatgcagg tgctgcggaa caacgagatg cagaagttca aggagatcca 4441 gacagagagg agggccacgg tgctagtcct ggttgtgctg ctgctattca tcatctgctg 4501 gctgcccttc cagatcagca ccttcctgga tacgctgcat cgcctcggca tcctctccag 4561 ctgccaggac gagcgcatca tcgatgtaat cacacagatc gcctccttca tggcctacag 4621 caacagctgc ctcaacccac tggtgtacgt gatcgtgggc aagcgcttcc gaaagaagtc 4681 ttgggaggtg taccagggag tgtgccagaa agggggctgc aggtcagaac ccattcagat 4741 ggagaactcc atgggcacac tgcggacctc catctccgtg gaacgccaga ttcacaaact 4801 gcaggactgg gcagggagca gacagTCTAG AGACTACAAA GACCATGACG GTGATTATAA 4861 AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGtag cggccgcgga tcccgcccct 4921 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt 4981 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac 5041 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc 5101 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa 5161 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg 5221 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg 5281 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc 5341 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat 5401 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg 5461 tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccacaagga gacgaccttc 5521 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt 5581 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga 5641 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 5701 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 5761 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 5821 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 5881 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 5941 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 6001 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 6061 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta 6121 gacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 6181 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 6241 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 6301 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 6361 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 6421 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 6481 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 6541 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 6601 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 6661 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 6721 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa 6781 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg 6841 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct 6901 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca 6961 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta 7021 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc 7081 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc 7141 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag 7201 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg 7261 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg 7321 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta 7381 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 7441 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 7501 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 7561 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 7621 cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg 7681 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 7741 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 7801 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 7861 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 7921 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 7981 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 8041 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 8101 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 8161 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 8221 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 8281 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 8341 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 8401 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 8461 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 8521 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 8581 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 8641 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 8701 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 8761 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 8821 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 8881 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 8941 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 9001 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 9061 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 9121 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 9181 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 9241 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 9301 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 9361 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 9421 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 9481 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 9541 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 9601 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 9661 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 9721 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 9781 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 9841 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa 9901 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 9961 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac 10021 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt 10081 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt 10141 taaatatgta ctacaaactt agtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.