pLV2-RNF187-3×HA-Puro plasmid

PVT100516

pLV2-RNF187-3×HA-Puro plasmid Information

Plasmid name: pLV2-RNF187(human-opt-human)-3×HA-Puro
Alias: NM_001010858.3;RACO-1; RACO1
Gene lenght: 705bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-RNF187-3×HA-Puro plasmid Description

pLV2-RNF187-3×HA-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-RNF187-3×HA-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccaTGGCTCT GCCTGCTGGA CCTGCTGAAG CTGCTTGCGC 2881 TCTGTGTCAG AGGGCCCCTA GAGAGCCTGT GAGGGCTGAT TGCGGCCACA GATTCTGTAG 2941 GGCCTGTGTG GTGAGGTTCT GGGCCGAAGA GGATGGCCCA TTCCCCTGTC CTGAGTGCGC 3001 TGATGACTGT TGGCAGAGGG CCGTGGAGCC TGGAAGACCT CCTCTGTCTA GAAGGCTGCT 3061 GGCCCTGGAG GAGGCTGCTG CTGCTCCTGC TAGAGACGGA CCTGCTAGCG AGGCTGCTCT 3121 GCAACTGCTG TGCAGAGCCG ACGCTGGACC ACTGTGTGCT GCTTGTAGAA TGGCCGCCGG 3181 CCCTGAACCT CCTGAATGGG AGCCTAGATG GAGGAAGGCC CTGAGGGGAA AGGAGAACAA 3241 GGGCAGCGTG GAGATCATGA GAAAGGATCT GAATGACGCC AGAGACCTGC ACGGCCAGGC 3301 TGAGAGCGCT GCTGCTGTGT GGAAGGGACA CGTGATGGAC AGAAGGAAGA AGGCCCTGAC 3361 CGACTACAAG AAGCTGAGAG CCTTTTTTGT GGAGGAGGAG GAGCACTTTC TGCAGGAGGC 3421 CGAGAAGGAG GAGGGCCTGC CTGAGGACGA ACTGGCTGAT CCAACAGAGA GGTTTAGAAG 3481 CCTGCTGCAG GCCGTGAGCG AGCTGGAGAA AAAGCACAGG AACCTGGGCC TGTCCATGCT 3541 GCTGCAGgga tccTACCCAT ACGACGTACC TGATTATGCA ACTTATCCTT ATGATGTACC 3601 TGATTATGCT AGCTATCCTT ATGATGTCCC AGACTACGCA TAGtctagat aattctaccg 3661 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 3721 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 3781 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 3841 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 3901 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 3961 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 4021 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 4081 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 4141 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg 4201 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 4261 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 4321 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 4381 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 4441 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 4501 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 4561 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 4621 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 4681 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 4741 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc 4801 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 4861 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 4921 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 4981 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 5041 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 5101 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 5161 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 5221 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 5281 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 5341 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt 5401 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat 5461 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta 5521 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 5581 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc 5641 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga 5701 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa 5761 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat 5821 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg 5881 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga 5941 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc 6001 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca 6061 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 6121 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 6181 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 6241 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca 6301 ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 6361 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 6421 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 6481 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 6541 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 6601 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 6661 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 6721 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 6781 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 6841 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 6901 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 6961 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 7021 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 7081 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 7141 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 7201 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 7261 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 7321 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 7381 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 7441 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 7501 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 7561 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 7621 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 7681 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 7741 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 7801 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 7861 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 7921 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 7981 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 8041 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 8101 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 8161 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 8221 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 8281 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 8341 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 8401 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 8461 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 8521 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 8581 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 8641 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc 8701 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat 8761 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta 8821 gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???