pLV2-TRE3GS-Kit-3×FLAG-TetOne-CopGFP-Neo plasmid

PVT110631

pLV2-TRE3GS-Kit-3×FLAG-TetOne-CopGFP-Neo plasmid Information

Plasmid name: pLV2-TRE3GS-Kit(mouse)-3×FLAG-TetOne-CopGFP-Neo
Alias: NM_001122733;W; Bs; Fdc; Ssm; SCO1; SCO5; SOW3; CD117; c-KIT; Tr-kit; Gsfsco1; Gsfsco5; Gsfsow3
Gene lenght: 2940
Host: Mammalian cells,lentivirus
Use(s): Gene expression,Tetracycline induction
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: TRE3GS
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: TRE3GS-F1
Reverse primer:
Copy number: High
Induction: Tetracycline induction

pLV2-TRE3GS-Kit-3×FLAG-TetOne-CopGFP-Neo plasmid Description

pLV2-TRE3GS-Kit-3×FLAG-TetOne-CopGFP-Neo Plasmid suitable for gene expression in mammalian cells, tetracycline-inducible, also suitable for lentivirus packaging.

pLV2-TRE3GS-Kit-3×FLAG-TetOne-CopGFP-Neo plasmid Sequence

1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg tTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 4141 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 4201 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGGT CCGCCACACC CAGCCGGCCA 4261 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 4321 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCGCG CCTTGAGCCT GGCGAACAGT 4381 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 4441 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 4501 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 4561 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 4621 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 4681 CACGATAGCC GCGCTGCCTC GTCCTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 4741 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 4801 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 4861 GCGTGCAATC CATCTTGTTC AATCATggtc gcgtCagggc cgggattctc ctccacgtca 4921 ccgcatgtta gaagacttcc tctgccctcg cgagatccgg tggagccggg tccggcggtg 4981 ccgtccacgg cagaattgga cgactgagcg cgggatctgg cgaaggcgat gggggtcttg 5041 aaggcgtgct ggtactccac gatgcccagc tcggtgttgc tgtgcagctc ctccacgcgg 5101 cggaaggcga acatggggcc cccgttctgc aggatgctgg ggtggatggc gctcttgaag 5161 tgcatgtggc tgtccaccac gaagctgtag tagccgccgt cgcgcaggct gaaggtgcgg 5221 gcgaagctgc ccaccagcac gttatcgccc atggggtgca ggtgctccac ggtggcgttg 5281 ctgcggatga tcttgtcggt gaagatcacg ctgtcctcgg ggaagccggt gcccaccacc 5341 ttgaagtcgc cgatcacgcg gccggcctcg tagcggtagc tgaagctcac gtgcagcacg 5401 ccgccgtcct cgtacttctc gatgcgggtg ttggtgtagc cgccgttgtt gatggcgtgc 5461 aggaaggggt tctcgtagcc gctggggtag gtgccgaagt ggtagaagcc gtagcccatc 5521 acgtggctca gcaggtaggg gctgaaggtc agggcgcctt tggtgctctt catcttgttg 5581 gtcatgcggc cctgcttggg ggtgccctct ccgccgccca ccagctcgaa ctccacgccg 5641 ttcagggtgc cggtgatgcg gcactcgatc tccatggcgg gcaggccgct ctcgtcgctc 5701 tccatggtgg cgtctagcgt aggcgccggt cacatttgca aaagcctagg cctccaaaaa 5761 agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa 5821 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg 5881 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca 5941 tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac taattgagat 6001 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac 6061 acacattcgc gcgtttaccc ggggagcatg tcaaggtcaa aatcgtcaag agcgtcagca 6121 ggcagcatat caaggtcaaa gtcgtcaagg gcatcggctg ggagcatgtc taagtcaaaa 6181 tcgtcaaggg cgtcggtcgg cccgccgctt tcgcacttta gctgtttctc caggccacat 6241 atgattagtt ccaggccgaa aaggaaggca ggttcggctc cctgccggtc gaacagctca 6301 attgcttgtt tcagaagtgg gggcatagaa tcggtggtag gtgtctctct ttcctctttt 6361 gctacttgat gctcctgttc ctccaatacg cagcccagtg taaagtggcc cacggcggac 6421 agagcgtaca gtgcgttctc cagggagaag ccttgctgac acaggaacgc gagctgattt 6481 tccagggttt cgtactgttt ctctgttggg cgggtgccga gatgcacttt agccccgtcg 6541 cgatgtgaga ggagagcaca gcggtatgac ttggcgttgt tccgcagaaa gtcttgccat 6601 gactcgcctt ccagggggca ggagtgggta tgatgcctgt ccagcatctc gattggcagg 6661 gcatcgagca gggcccgctt gttcttcacg tgccagtaca gggtaggctg ctcaactccc 6721 agcttttgag cgagtttcct tgtcgtcagg ccttcgatac cgactccatt gagtaattcc 6781 agagcagagt ttatgacttt gctcttgtcc agtctagaca tggtaattcg atgatccccc 6841 tggggagaga ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga 6901 ggcttgcaga atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac 6961 cccgcctccc gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg 7021 ctattggcca cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct 7081 gcgagggtac tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc 7141 aaggaacctt cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta 7201 gcggcgaaga tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg 7261 gcgccgctgc gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg 7321 ctgccttgga aaaggcgcaa ccccaacccc gtggaattat cacctcgagt ttactcccta 7381 tcagtgatag agaacgtatg aagagtttac tccctatcag tgatagagaa cgtatgcaga 7441 ctttactccc tatcagtgat agagaacgta taaggagttt actccctatc agtgatagag 7501 aacgtatgac cagtttactc cctatcagtg atagagaacg tatctacagt ttactcccta 7561 tcagtgatag agaacgtata tccagtttac tccctatcag tgatagagaa cgtataagct 7621 ttgcttatgt aaaccagggc gcctataaaa gagtgctgat tttttgagta aacttcaatt 7681 ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg taaagaattc 7741 ccgtatacac cggtGCCACC atgagaggcg ctcgcggcgc ctgggatctg ctctgcgtcc 7801 tgttggtcct gctccgtggc cagacagcca cgtctcagcc atctgcaagt ccaggggagc 7861 cgtctccgcc atccatccat ccagcacaat cagagttaat agttgaagct ggcgacaccc 7921 tcagcctgac gtgcattgat cccgactttg tcagatggac tttcaagacc tatttcaatg 7981 aaatggttga gaataaaaaa aatgaatgga tccaggaaaa agccgaggcc actcgcacgg 8041 gcacatacac gtgcagcaac agcaatggcc tcacgagttc tatttacgtg tttgttagag 8101 atcctgccaa acttttcctg gttggccttc ccttgtttgg caaagaagac agcgacgcgc 8161 tggtccgctg ccctctgaca gacccacagg tgtccaatta ttccctcatc gagtgtgatg 8221 ggaaatctct ccccacggac ctgacgtttg tcccaaaccc caaggctggc atcaccatca 8281 aaaacgtgaa gcgcgcctac caccggctct gtgtccgctg tgctgctcag cgtgacggta 8341 catggctgca ttctgacaaa ttcaccctca aagtgcgggc agccatcaag gctatccctg 8401 ttgtgtctgt gcctgaaaca agtcacctcc ttaagaaagg ggacacattt acggtggtgt 8461 gcaccataaa agatgtgtct acatccgtga actccatgtg gctaaagatg aaccctcagc 8521 ctcagcacat agcccaggta aagcacaata gctggcaccg gggtgacttc aattatgaac 8581 gccaggagac gctgactatc agctcggcaa gagttgacga ttctggagtg ttcatgtgtt 8641 atgccaataa tacttttgga tcagcaaatg tcacaacaac cttgaaagta gtagaaaaag 8701 gattcatcaa catctcccct gtgaagaaca ctacagtatt tgtaaccgat ggagaaaacg 8761 tagatttggt tgttgaatac gaggcctacc ccaaacccga gcaccagcag tggatatata 8821 tgaacaggac ctcggctaac aaagggaagg attatgtcaa atctgataac aaaagcaaca 8881 tcagatatgt gaaccaactt cgcctgacca gattaaaagg cacagaagga ggcacttata 8941 cctttctggt gtccaactct gatgccagtg cttccgtgac attcaacgtt tacgtgaaca 9001 caaaaccaga aatcctgacg tacgacaggc tcataaatgg catgctccag tgtgtggcag 9061 agggattccc ggagcccaca atagattggt atttttgtac aggagcagag caaaggtgta 9121 ccactcctgt ctcaccagtg gacgtacagg tccagaatgt atctgtgtca ccatttggaa 9181 aactggtggt tcagagttcc atagactcca gcgtcttccg gcacaacggc acggtggagt 9241 gtaaggcctc caacgatgtg ggcaagagtt ccgccttctt taactttgca tttaaaggta 9301 acaacaaaga gcaaatccag gcccacactc tgttcacgcc gctgctcatt ggctttgtgg 9361 ttgcagctgg cgcgatgggg atcattgtga tggtgctcac ctacaaatat ttgcagaaac 9421 ccatgtatga agtacaatgg aaggttgtcg aggagataaa tggaaacaat tatgtttaca 9481 tagacccgac gcaacttcct tatgatcaca aatgggagtt tcccagaaac aggctgagtt 9541 ttggaaagac attgggagct ggtgccttcg ggaaggtcgt tgaggccact gcatatggct 9601 tgattaagtc ggatgctgcc atgacagttg ccgtgaagat gctcaaacca agtgcccatt 9661 taacagaaag agaggcccta atgtcggaac tgaaggtcct gagctacctg ggcaatcaca 9721 tgaatattgt gaacctgctt ggcgcatgca cggtgggagg gcccaccctg gtcattacag 9781 aatattgttg ctatggtgat cttttgaatt ttttgagaag gaagcgtgac tcgtttattt 9841 tctcaaagca agaagagcag gcagaagcgg cactttataa gaaccttctg cactcaacgg 9901 agccttcctg tgacagttca aatgaatata tggacatgaa gcctggcgtt tcctacgtgg 9961 tgccaaccaa gacagacaag aggagatccg caagaataga ctcgtacata gaaagagacg 10021 tgactcctgc catcatggaa gatgacgagc tggctctgga cctggatgat ttgctgagct 10081 tctcctacca ggtggccaag ggcatggcgt tcctcgcctc caagaattgt attcacagag 10141 atttggcagc caggaatatc ctcctcactc acgggcggat cacaaagatt tgcgatttcg 10201 ggctagccag agacatcagg aatgattcga attacgtggt caaaggaaat gcacgactgc 10261 ccgtgaagtg gatggcacca gagagcattt tcagctgcgt gtacacattt gaaagtgatg 10321 tctggtccta tgggattttc ctctgggagc tcttctcctt aggaagcagc ccctacccag 10381 ggatgccggt cgactccaag ttctacaaga tgatcaagga aggcttccgg atggtcagcc 10441 cggagcacgc gcctgccgaa atgtatgacg tcatgaagac ttgctgggac gctgacccct 10501 tgaaaaggcc aacattcaag caggttgtcc aacttattga gaagcagatc tcggacagca 10561 ccaagcacat ttactccaac ttggcaaact gcaaccccaa cccagagaac cccgtggtgg 10621 tggaccattc cgtgagggtc aactcggtgg gcagcagcgc ctcttctacg cagcccctgc 10681 tcgtgcacga agatgccGGA TCCGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG 10741 ACATCGACTA CAAGGATGAC GATGACAAGT AGagaccacc tcccctgcga gctaagctgg 10801 acagccaatg acgggtaaga gagtgacatt tttcactaac ctaagacagg agggccgtca 10861 gagctactgc ctaatccaaa gacgggtaaa agtgataaaa atgtatcact ccaacctaag 10921 acaggcgcag cttccgaggg atttgagatc cagacatgat aagatacatt gatgagtttg 10981 gacaaaccaa aactagaatg cagtgaaaaa aatgccttat ttgtgaaatt tgtgatgcta 11041 ttgccttatt tgtaaccatt ataagctgca ataaacaagt taagtcgata aactggatct 11101 ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt ttgtagttct 11161 ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca 11221 atcccccctt ttcttttaaa ggcgataccg tcgagatccg ttcactaatc gaatggatct 11281 gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg 11341 ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat 11401 agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa 11461 ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc 11521 taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga 11581 gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt 11641 ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag cagtggtgca 11701 aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc 11761 cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg 11821 ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa 11881 ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc 11941 caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct 12001 tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata 12061 taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg 12121 gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct 12181 tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta 12241 tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt 12301 tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc 12361 ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga 12421 attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct 12481 ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg 12541 cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc 12601 cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa 12661 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa 12721 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct 12781 cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat 12841 atgcaggatc tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag 12901 tccccagcgg aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga 12961 tgcagctctc gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact 13021 tctctctccg ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct 13081 cctttattgg cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca 13141 aaggtcagtg gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag 13201 ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc ttctttggga gtgaattagc 13261 ccttcca

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.