pLV2-TRE3GS-Neurog2-3×FLAG-TetOne-Puro plasmid

PVT111904

pLV2-TRE3GS-Neurog2-3×FLAG-TetOne-Puro plasmid Information

Plasmid name: pLV2-TRE3GS-Neurog2(mouse)-3×FLAG-TetOne-Puro
Alias: NM_009718.4;Atoh4; bHLHa8; Math4A; ngn-2; ngn2
Gene lenght: 789
Host: Mammalian cells,lentivirus
Use(s): Gene expression,Tetracycline induction
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: TRE3GS
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: TRE3GS-F1
Reverse primer:
Copy number: High
Induction: Tetracycline induction

pLV2-TRE3GS-Neurog2-3×FLAG-TetOne-Puro plasmid Description

pLV2-TRE3GS-Neurog2-3×FLAG-TetOne-Puro is a plasmid suitable for gene expression in mammalian cells, tetracycline-inducible, and can also be used for lentivirus packaging.

pLV2-TRE3GS-Neurog2-3×FLAG-TetOne-Puro plasmid Sequence

1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc 4141 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc 4201 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc 4261 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg 4321 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg 4381 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc 4441 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac 4501 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc 4561 tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg 4621 gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg 4681 tactcggtca tggtcgcgtt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact 4741 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc 4801 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta 4861 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 4921 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac 4981 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt 5041 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg 5101 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg 5161 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg 5221 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga 5281 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc 5341 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg 5401 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac 5461 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga 5521 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg 5581 gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc 5641 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt 5701 ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc agagtttatg 5761 actttgctct tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg 5821 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg 5881 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc 5941 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc 6001 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg 6061 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga 6121 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg 6181 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc 6241 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg 6301 cgcaacccca accccgtgga attatcacct cgagtttact ccctatcagt gatagagaac 6361 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca 6421 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt 6481 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac 6541 gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc 6601 agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt 6661 cttataccaa ctttccgtac cacttcctac cctcgtaaag aattcgccac catgttcgtc 6721 aaatctgaga ctctggagtt gaaggaggaa gaggaggtac tgatgctgct gggctcggct 6781 tccccggcct cggcgaccct gaccccgatg tcctccagcg cggacgagga ggaggacgag 6841 gagctgcgcc ggccgggctc cgcgcgtggg cagcgtggag cggaagccgg gcagggggtg 6901 cagggcagtc cggcgtcggg tgccgggggt tgccggccag ggcggctgct gggcctgatg 6961 cacgagtgca agcgtcgccc gtcgcgctca cgggccgtct cccgaggtgc caagacggcg 7021 gagacggtgc agcgcatcaa gaagacccgc aggctcaagg ccaacaaccg cgagcgcaac 7081 cgcatgcaca acctaaacgc cgcgctggac gcgctgcgcg aggtgctgcc caccttcccc 7141 gaggatgcca agctcacgaa gatcgagacg ctgcgcttcg cccacaatta catctgggcg 7201 ctcaccgaga ctctgcgcct ggcggaccac tgcgccggcg ccggtggcct ccagggggcg 7261 ctcttcacgg aggcggtgct cctgagcccg ggagctgcgc tcggcgccag cggggacagc 7321 ccttctccac cttcctcctg gagctgcacc aacagcccgg cgtcatcctc caactccacg 7381 tccccataca gctgcacttt atcgcccgct agccccgggt cagacgtgga ctactggcag 7441 cccccacctc cggagaagca tcgttatgcg cctcacctgc ccctcgccag ggactgtatc 7501 TTCGAAGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT 7561 GACGATGACA AGTAGggatc cagaccacct cccctgcgag ctaagctgga cagccaatga 7621 cgggtaagag agtgacattt ttcactaacc taagacagga gggccgtcag agctactgcc 7681 taatccaaag acgggtaaaa gtgataaaaa tgtatcactc caacctaaga caggcgcagc 7741 ttccgaggga tttgagatcc agacatgata agatacattg atgagtttgg acaaaccaaa 7801 actagaatgc agtgaaaaaa atgccttatt tgtgaaattt gtgatgctat tgccttattt 7861 gtaaccatta taagctgcaa taaacaagtt aagtcgataa actggatctc tgctgtccct 7921 gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtagttctt tagtttgtat 7981 gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt 8041 tcttttaaag gcgataccgt cgagatccgt tcactaatcg aatggatctg tctctgtctc 8101 tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt 8161 gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata gaaagtacag 8221 caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc 8281 acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata 8341 attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc 8401 ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt 8461 ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc 8521 cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga 8581 ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct 8641 caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct 8701 gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa 8761 ggaacaaagc tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct 8821 tggtgggtgc tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc 8881 tccaattgtc cctcatatct cctcctccag gtctgaagat cagcggccgg ccgcttgctg 8941 tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc 9001 ttttatctct atcctttgat gcacacaata gagggttgct actgtattat ataatgatct 9061 aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc 9121 ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca 9181 tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca 9241 tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta 9301 gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt 9361 gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt 9421 caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg 9481 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc 9541 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag 9601 atctggtcta accagagaga cccagtacag gcaaaaagca gctgcttata tgcaggatct 9661 gagggctcgc cactccccag tcccgcccag gccacgcctc cctggaaagt ccccagcgga 9721 aagtcccttg tagcaagctc gatatcagca gttcttgaag tactccggat gcagctctcg 9781 ggccacgtga tgaaatgcta ggcggctgtc aaacctccac tctaacactt ctctctccgg 9841 gtcatccatc ccatgcaggc tcacagggtg taacaagctg gtgttctctc ctttattggc 9901 ctcttctacc ttatctggct caactggtac tagcttgtag caccatccaa aggtcagtgg 9961 atatctgacc cctggccctg gtgtgtagtt ctgctaatca gggaagtagc cttgtgtgtg 10021 gtagatccac agatcaagga tatcttgtct tctttgggag tgaattagcc cttcca

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.