pLV2-U6-HDAC6-shRNA2-Puro plasmid

PVT93845

pLV2-U6-HDAC6-shRNA2-Puro plasmid Information

Plasmid name: pLV2-U6-HDAC6(human)-shRNA2-Puro
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV2-U6-HDAC6-shRNA2-Puro plasmid Description

pLV2-U6-HDAC6-shRNA2-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-U6-HDAC6-shRNA2-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatctgggc aggaagaggg cctatttccc atgattcctt 2221 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa 2281 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg 2341 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt 2401 tcgatttctt ggctttatat atcttgtgga aaggacgagg atccgTGGTT CACAGCCTAG 2461 AATATAttca agagaTATAT TCTAGGCTGT GAACCAtttt ttgaattctC TAGATAATTC 2521 Taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg 2581 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt 2641 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta 2701 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta 2761 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc 2821 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga 2881 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg 2941 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc 3001 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc 3061 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg 3121 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg 3181 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 3241 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 3301 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 3361 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 3421 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 3481 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 3541 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg 3601 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc 3661 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc ggcgtctcga gtctagaggg 3721 cccgacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 3781 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 3841 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 3901 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 3961 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 4021 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 4081 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt 4141 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 4201 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 4261 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 4321 ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag 4381 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt 4441 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga 4501 tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag 4561 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa 4621 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt 4681 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca 4741 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga 4801 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg 4861 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct 4921 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct 4981 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 5041 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc 5101 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 5161 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa 5221 tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct agctagagct 5281 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 5341 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 5401 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 5461 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 5521 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 5581 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 5641 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 5701 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 5761 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 5821 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 5881 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 5941 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 6001 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 6061 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 6121 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 6181 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 6241 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 6301 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 6361 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 6421 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 6481 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 6541 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 6601 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 6661 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 6721 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 6781 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 6841 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 6901 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 6961 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 7021 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 7081 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 7141 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 7201 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 7261 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 7321 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 7381 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 7441 cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa 7501 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 7561 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct 7621 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg 7681 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt 7741 tgttaaatat gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt taaatatgta 7801 ctacaaactt agtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???