pLV2-YME1L1-3×FLAG-IRES-Puro plasmid

PVT99326

pLV2-YME1L1-3×FLAG-IRES-Puro plasmid Information

Plasmid name: pLV2-YME1L1(human)-3×FLAG-IRES-Puro
Alias: NM_139312.3;FTSH; MEG4; OPA11; PAMP; YME1L
Gene lenght: 2319bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-YME1L1-3×FLAG-IRES-Puro plasmid Description

pLV2-YME1L1-3×FLAG-IRES-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-YME1L1-3×FLAG-IRES-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgttt 2821 tccttgtcga gcacggtgca accccaggtt acagttcctc tgagtcatct catcaatgcc 2881 ttccatacac caaaaaacac ttctgtttct ctcagtggag tgtcagtttc tcaaaaccag 2941 catcgagatg tagttcctga gcatgaggct cccagcagtg agtgtatgtt cagtgacttc 3001 ctgacgaagc ttaacattgt ttcaattggc aaaggaaaaa tattcgaagg gtacagatcc 3061 atgttcatgg agccagcaaa aaggatgaag aagagcttgg acacaaccga taactggcac 3121 atccgtccag aacccttctc cctctcaatc cctccttcac ttaacttaag ggaccttgga 3181 ttatctgaac taaaaattgg acagattgat cagctggtag aaaatctact tcctggattt 3241 tgtaaaggca aaaacatttc ttcccattgg catacatccc atgtctctgc acaatccttc 3301 tttgaaaata aatatggtaa cttagatata tttagtacat tacgttcctc ttgcttgtat 3361 cgacatcatt caagagctct tcaaagcatt tgttcagatc ttcagtactg gccagttttc 3421 atacagtctc ggggttttaa aactttgaaa tcaaggacac gacgtctcca gtctacctcc 3481 gagagattag ctgaaacaca gaatatagcg ccatcattcg tgaaggggtt tcttttgcgg 3541 gacagaggat cagatgttga gagtttggac aaactcatga aaaccaaaaa tatacctgaa 3601 gctcaccaag atgcatttaa aactggtttt gcggaaggtt ttctgaaagc tcaagcactc 3661 acacaaaaaa ccaatgattc cctaaggcga acccgtctga ttctcttcgt tctgctgcta 3721 ttcggcattt atggacttct aaaaaaccca tttttatctg tccgcttccg gacaacaaca 3781 gggcttgatt ctgcagtaga tcctgtccag atgaaaaatg tcacctttga acatgttaaa 3841 ggggtggagg aagctaaaca agaattacag gaagttgttg aattcttgaa aaatccacaa 3901 aaatttacta ttcttggagg taaacttcca aaaggaattc ttttagttgg acccccaggg 3961 actggaaaga cacttcttgc ccgagctgtg gcgggagaag ctgatgttcc tttttattat 4021 gcttctggat ccgaatttga tgagatgttt gtgggtgtgg gagccagccg tatcagaaat 4081 ctttttaggg aagcaaaggc gaatgctcct tgtgttatat ttattgatga attagattct 4141 gttggtggga agagaattga atctccaatg catccatatt caaggcagac cataaatcaa 4201 cttcttgctg aaatggatgg ttttaaaccc aatgaaggag ttatcataat aggagccaca 4261 aacttcccag aggcattaga taatgcctta atacgtcctg gtcgttttga catgcaagtt 4321 acagttccaa ggccagatgt aaaaggtcga acagaaattt tgaaatggta tctcaataaa 4381 ataaagtttg atcaatccgt tgatccagaa attatagctc gaggtactgt tggcttttcc 4441 ggagcagagt tggagaatct tgtgaaccag gctgcattaa aagcagctgt tgatggaaaa 4501 gaaatggtta ccatgaagga gctggagttt tccaaagaca aaattctaat ggggcctgaa 4561 agaagaagtg tggaaattga taacaaaaac aaaaccatca cagcatatca tgaatctggt 4621 catgccatta ttgcatatta cacaaaagat gcaatgccta tcaacaaagc tacaatcatg 4681 ccacgggggc caacacttgg acatgtgtcc ctgttacctg agaatgacag atggaatgaa 4741 actagagccc agctgcttgc acaaatggat gttagtatgg gaggaagagt ggcagaggag 4801 cttatatttg gaaccgacca tattacaaca ggtgcttcca gtgattttga taatgccact 4861 aaaatagcaa agcggatggt taccaaattt ggaatgagtg aaaagcttgg agttatgacc 4921 tacagtgata cagggaaact aagtccagaa acccaatctg ccatcgaaca agaaataaga 4981 atccttctaa gggactcata tgaacgagca aaacatatct tgaaaactca tgcaaaggag 5041 cataagaatc tcgcagaagc tttattgacc tatgagactt tggatgccaa agagattcaa 5101 attgttcttg aggggaaaaa gttggaagtg agaGACTACA AAGACCATGA CGGTGATTAT 5161 AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGT AGggatcccg cccctctccc 5221 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc 5281 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc 5341 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt 5401 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct 5461 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa 5521 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt 5581 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag 5641 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt 5701 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt 5761 ttcctttgaa aaacacgatg ataagcttgc cacaacccac aaggagacga ccttccatga 5821 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca 5881 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc 5941 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg 6001 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg 6061 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc 6121 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg 6181 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg 6241 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga 6301 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg 6361 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctagacgc 6421 gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 6481 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 6541 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 6601 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 6661 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 6721 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 6781 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca 6841 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 6901 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 6961 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct 7021 ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag 7081 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc 7141 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 7201 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata 7261 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac 7321 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg 7381 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga 7441 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc 7501 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc 7561 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga 7621 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg 7681 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 7741 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 7801 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 7861 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 7921 agtgagaggc cttgacattg ctagcgttta ccgtcgacct ctagctagag cttggcgtaa 7981 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 8041 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 8101 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 8161 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 8221 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 8281 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 8341 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 8401 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 8461 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 8521 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 8581 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 8641 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 8701 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 8761 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 8821 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 8881 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 8941 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 9001 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 9061 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 9121 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 9181 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 9241 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 9301 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 9361 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 9421 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 9481 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 9541 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 9601 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 9661 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 9721 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 9781 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 9841 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 9901 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 9961 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 10021 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 10081 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 10141 gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa 10201 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 10261 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa 10321 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt 10381 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat 10441 atgtactaca aacttagtag tttttaaaga aattgtattt gttaaatatg tactacaaac 10501 ttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???