pLV3-CAG-PAH-3×HA-EF1a-Fluc-Puro plasmid

PVT90895

pLV3-CAG-PAH-3×HA-EF1a-Fluc-Puro plasmid Information

Plasmid name: pLV3-CAG-PAH(human)-3×HA-EF1a-Fluc-Puro
Alias: NM_000277.3;PH; PKU; PKU1
Gene lenght: 1356bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CAG
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: pcaggs-F
Reverse primer:
Copy number: High
Induction:

pLV3-CAG-PAH-3×HA-EF1a-Fluc-Puro plasmid Description

pLV3-CAG-PAH-3×HA-EF1a-Fluc-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CAG-PAH-3×HA-EF1a-Fluc-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt 1981 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga 2041 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca 2101 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca 2161 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg 2221 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc 2281 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct 2341 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg 2401 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg 2461 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa 2521 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc 2581 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc 2641 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc 2701 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg 2761 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg 2821 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc 2881 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg 2941 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg 3001 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca 3061 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg 3121 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc 3181 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg 3241 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc 3301 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac 3361 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga 3421 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc 3481 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt 3541 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagtc 3601 tagagctagc gaattcGCCA CCatgtccac tgcggtcctg gaaaacccag gcttgggcag 3661 gaaactctct gactttggac aggaaacaag ctatattgaa gacaactgca atcaaaatgg 3721 tgccatatca ctgatcttct cactcaaaga agaagttggt gcattggcca aagtattgcg 3781 cttatttgag gagaatgatg taaacctgac ccacattgaa tctagacctt ctcgtttaaa 3841 gaaagatgag tatgaatttt tcacccattt ggataaacgt agcctgcctg ctctgacaaa 3901 catcatcaag atcttgaggc atgacattgg tgccactgtc catgagcttt cacgagataa 3961 gaagaaagac acagtgccct ggttcccaag aaccattcaa gagctggaca gatttgccaa 4021 tcagattctc agctatggag cggaactgga tgctgaccac cctggtttta aagatcctgt 4081 gtaccgtgca agacggaagc agtttgctga cattgcctac aactaccgcc atgggcagcc 4141 catccctcga gtggaataca tggaggaaga aaagaaaaca tggggcacag tgttcaagac 4201 tctgaagtcc ttgtataaaa cccatgcttg ctatgagtac aatcacattt ttccacttct 4261 tgaaaagtac tgtggcttcc atgaagataa cattccccag ctggaagacg tttctcagtt 4321 cctgcagact tgcactggtt tccgcctccg acctgtggct ggcctgcttt cctctcggga 4381 tttcttgggt ggcctggcct tccgagtctt ccactgcaca cagtacatca gacatggatc 4441 caagcccatg tatacccccg aacctgacat ctgccatgag ctgttgggac atgtgccctt 4501 gttttcagat cgcagctttg cccagttttc ccaggaaatt ggccttgcct ctctgggtgc 4561 acctgatgaa tacattgaaa agctcgccac aatttactgg tttactgtgg agtttgggct 4621 ctgcaaacaa ggagactcca taaaggcata tggtgctggg ctcctgtcat cctttggtga 4681 attacagtac tgcttatcag agaagccaaa gcttctcccc ctggagctgg agaagacagc 4741 catccaaaat tacactgtca cggagttcca gcccctctat tacgtggcag agagttttaa 4801 tgatgccaag gagaaagtaa ggaactttgc tgccacaata cctcggccct tctcagttcg 4861 ctacgaccca tacacccaaa ggattgaggt cttggacaat acccagcagc ttaagatttt 4921 ggctgattcc attaacagtg aaattggaat cctttgcagt gccctccaga aaataaagTA 4981 CCCATACGAC GTACCTGATT ATGCAACTTA TCCTTATGAT GTACCTGATT ATGCTAGCTA 5041 TCCTTATGAT GTCCCAGACT ACGCATAGgg atccgcggcc gcaaggatct gcgatcgctc 5101 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 5161 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 5221 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 5281 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 5341 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 5401 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 5461 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 5521 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 5581 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac 5641 catggagagc gacgagagcg gcctgatgga agacgccaaa aacataaaga aaggcccggc 5701 gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag 5761 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac 5821 ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct 5881 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt 5941 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg 6001 tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg 6061 gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat 6121 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct 6181 acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat 6241 tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca 6301 tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat 6361 tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac 6421 actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct 6481 gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt 6541 ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat 6601 tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca 6661 tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac 6721 acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa 6781 ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt 6841 gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat 6901 tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt 6961 cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc 7021 tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct 7081 tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac 7141 gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt 7201 gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc 7261 aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgagatc 7321 ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc 7381 tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat 7441 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 7501 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 7561 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 7621 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 7681 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 7741 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 7801 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 7861 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 7921 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 7981 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa 8041 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 8101 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 8161 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 8221 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 8281 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 8341 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 8401 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 8461 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 8521 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg 8581 ccttcgccct cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag 8641 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 8701 ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct 8761 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 8821 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 8881 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 8941 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 9001 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 9061 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 9121 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct 9181 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 9241 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc 9301 ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 9361 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 9421 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 9481 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 9541 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 9601 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 9661 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 9721 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 9781 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 9841 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 9901 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 9961 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 10021 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 10081 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 10141 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 10201 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 10261 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 10321 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 10381 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 10441 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 10501 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 10561 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 10621 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 10681 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 10741 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 10801 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 10861 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 10921 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 10981 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 11041 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 11101 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 11161 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 11221 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 11281 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 11341 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 11401 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 11461 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 11521 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 11581 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 11641 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag 11701 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 11761 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg 11821 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta 11881 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag 11941 ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.