pLV3-CMV-3×HA-ASAH1-3×FLAG-CopGFP-Puro plasmid

PVT92196

pLV3-CMV-3×HA-ASAH1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-3×HA-ASAH1(human)-3×FLAG-CopGFP-Puro
Alias: NM_177924.5;AC; ACDase; ASAH; PHP; PHP32; SMAPME
Gene lenght: 1185bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×HA-ASAH1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-3×HA-ASAH1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-3×HA-ASAH1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgT ACCCATACGA CGTACCTGAT 2341 TATGCAACTT ATCCTTATGA TGTACCTGAT TATGCTAGCT ATCCTTATGA TGTCCCAGAC 2401 TACGCAatgc cgggccggag ttgcgtcgcc ttagtcctcc tggctgccgc cgtcagctgt 2461 gccgtcgcgc agcacgcgcc gccgtggaca gaggactgca gaaaatcaac ctatcctcct 2521 tcaggaccaa cgtacagagg tgcagttcca tggtacacca taaatcttga cttaccaccc 2581 tacaaaagat ggcatgaatt gatgcttgac aaggcaccag tgctaaaggt tatagtgaat 2641 tctctgaaga atatgataaa tacattcgtg ccaagtggaa aaattatgca ggtggtggat 2701 gaaaaattgc ctggcctact tggcaacttt cctggccctt ttgaagagga aatgaagggt 2761 attgccgctg ttactgatat acctttagga gagattattt cattcaatat tttttatgaa 2821 ttatttacca tttgtacttc aatagtagca gaagacaaaa aaggtcatct aatacatggg 2881 agaaacatgg attttggagt atttcttggg tggaacataa ataatgatac ctgggtcata 2941 actgagcaac taaaaccttt aacagtgaat ttggatttcc aaagaaacaa caaaactgtc 3001 ttcaaggctt caagctttgc tggctatgtg ggcatgttaa caggattcaa accaggactg 3061 ttcagtctta cactgaatga acgtttcagt ataaatggtg gttatctggg tattctagaa 3121 tggattctgg gaaagaaaga tgtcatgtgg atagggttcc tcactagaac agttctggaa 3181 aatagcacaa gttatgaaga agccaagaat ttattgacca agaccaagat attggcccca 3241 gcctacttta tcctgggagg caaccagtct ggggaaggtt gtgtgattac acgagacaga 3301 aaggaatcat tggatgtata tgaactcgat gctaagcagg gtagatggta tgtggtacaa 3361 acaaattatg accgttggaa acatcccttc ttccttgatg atcgcagaac gcctgcaaag 3421 atgtgtctga accgcaccag ccaagagaat atctcatttg aaaccatgta tgatgtcctg 3481 tcaacaaaac ctgtcctcaa caagctgacc gtatacacaa ccttgataga tgttaccaaa 3541 ggtcaattcg aaacttacct gcgggactgc cctgaccctt gtataggttg gggatccGAC 3601 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC 3661 AAGtagcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca 3721 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga 3781 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 3841 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 3901 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc 3961 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt 4021 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc 4081 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg 4141 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc 4201 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc 4261 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg 4321 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa 4381 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac 4441 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc 4501 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc 4561 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc 4621 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg 4681 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac 4741 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc 4801 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc 4861 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc 4921 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc 4981 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc 5041 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 5101 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 5161 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 5221 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 5281 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 5341 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 5401 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 5461 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 5521 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 5581 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 5641 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 5701 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 5761 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 5821 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 5881 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 5941 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 6001 ctgttgggca ctgacaattc cgtggtgttg tcggggaaAT CATCGTCCTT TCCTtggctg 6061 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 6121 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 6181 ctTcgccttc gccctcagac gagtcggatc tccctttggG ccgcctcccc gcctggtacc 6241 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 6301 gggactggaa gggctaattc actcccaacg aaGataagat ctgctttttg cttgtactgg 6361 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 6421 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 6481 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 6541 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 6601 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 6661 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 6721 caatgtatct tatcatgtct ggctctagct aTCCCGCCCC TAACTCCGCC CAtcccgccc 6781 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 6841 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 6901 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg 6961 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 7021 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 7081 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 7141 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 7201 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 7261 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 7321 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 7381 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 7441 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 7501 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 7561 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 7621 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 7681 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 7741 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 7801 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 7861 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 7921 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 7981 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 8041 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 8101 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 8161 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 8221 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 8281 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 8341 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 8401 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 8461 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 8521 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 8581 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 8641 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 8701 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 8761 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 8821 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 8881 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 8941 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 9001 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 9061 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 9121 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 9181 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 9241 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 9301 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 9361 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg 9421 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 9481 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 9541 taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???