pLV3-CMV-3×HA-GPR61-EF1a-CopGFP-Puro plasmid

PVT100514

pLV3-CMV-3×HA-GPR61-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-3×HA-GPR61(human)-EF1a-CopGFP-Puro
Alias: NM_031936.6BALGR; GPCR3
Gene lenght: 1353bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×HA-GPR61-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-3×HA-GPR61-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-3×HA-GPR61-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgT ACCCATACGA CGTACCTGAT 2341 TATGCAACTT ATCCTTATGA TGTACCTGAT TATGCTAGtT ATCCTTATGA TGTCCCAGAC 2401 TACGCAatgg agtcctcacc catcccccag tcatcaggga actcttccac tttggggagg 2461 gtccctcaaa ccccaggtcc ctctactgcc agtggggtcc cggaggtggg gctacgggat 2521 gttgcttcgg aatctgtggc cctcttcttc atgctcctgc tggacttgac tgctgtggct 2581 ggcaatgccg ctgtgatggc cgtgatcgcc aagacgcctg ccctccgaaa atttgtcttc 2641 gtcttccacc tctgcctggt ggacctgctg gctgccctga ccctcatgcc cctggccatg 2701 ctctccagct ctgccctctt tgaccacgcc ctctttgggg aggtggcctg ccgcctctac 2761 ttgtttctga gcgtgtgctt tgtcagcctg gccatcctct cggtgtcagc catcaatgtg 2821 gagcgctact attacgtagt ccaccccatg cgctacgagg tgcgcatgac gctggggctg 2881 gtggcctctg tgctggtggg tgtgtgggtg aaggccttgg ccatggcttc tgtgccagtg 2941 ttgggaaggg tctcctggga ggaaggagct cccagtgtcc ccccaggctg ttcactccag 3001 tggagccaca gtgcctactg ccagcttttt gtggtggtct ttgctgtcct ttactttctg 3061 ttgcccctgc tcctcatact tgtggtctac tgcagcatgt tccgagtggc ccgcgtggct 3121 gccatgcagc acgggccgct gcccacgtgg atggagacac cccggcaacg ctccgaatct 3181 ctcagcagcc gctccacgat ggtcaccagc tcgggggccc cccagaccac cccacaccgg 3241 acgtttgggg gagggaaagc agcagtggtt ctcctggctg tggggggaca gttcctgctc 3301 tgttggttgc cctacttctc tttccacctc tatgttgccc tgagtgctca gcccatttca 3361 actgggcagg tggagagtgt ggtcacctgg attggctact tttgcttcac ttccaaccct 3421 ttcttctatg gatgtctcaa ccggcagatc cggggggagc tcagcaagca gtttgtctgc 3481 ttcttcaagc cagctccaga ggaggagctg aggctgccta gccgggaggg ctccattgag 3541 gagaacttcc tgcagttcct tcaggggact ggctgtcctt ctgagtcctg ggtttcccga 3601 cccctaccca gccccaagca ggagccacct gctgttgact ttcgaatccc aggccagata 3661 gctgaggaga cctctgagtt cctggagcag caactcacca gcgacatcat catgtcagac 3721 agctacctcc gtcctgccgc ctcaccccgg ctggagtcaT AggatccCTG CGATATCCAG 3781 CgcggccgcG aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc 3841 ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt 3901 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg 3961 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg 4021 ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc 4081 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg 4141 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt 4201 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc 4261 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc 4321 tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc ctgcccgcca 4381 tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag ctggtgggcg 4441 gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc accaaaggcg 4501 ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc taccacttcg 4561 gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac ggcggctaca 4621 ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc 4681 gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc ttccccgagg 4741 acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag cacctgcacc 4801 ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg 4861 gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc caccccagca 4921 tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg cacagcaaca 4981 ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc ttcgccagat 5041 cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc tccaccggat 5101 ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta 5161 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac 5221 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc 5281 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 5341 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 5401 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 5461 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 5521 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 5581 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 5641 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 5701 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa 5761 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 5821 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 5881 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 5941 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 6001 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 6061 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 6121 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 6181 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 6241 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc 6301 gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg gtacctttaa 6361 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 6421 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 6481 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6541 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 6601 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 6661 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 6721 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 6781 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 6841 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 6901 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 6961 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 7021 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 7081 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 7141 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 7201 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 7261 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 7321 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 7381 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 7441 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 7501 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 7561 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 7621 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 7681 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 7741 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 7801 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 7861 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 7921 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 7981 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 8041 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 8101 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 8161 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 8221 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 8281 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 8341 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 8401 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 8461 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 8521 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 8581 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 8641 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 8701 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 8761 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 8821 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 8881 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 8941 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 9001 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 9061 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 9121 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 9181 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 9241 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 9301 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 9361 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 9421 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 9481 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 9541 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 9601 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 9661 gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???