pLV3-CMV-4×Mito-ADCY10-3×Myc-CopGFP-Puro plasmid

PVT90659

pLV3-CMV-4×Mito-ADCY10-3×Myc-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-4×Mito-ADCY10(human)-3×Myc-CopGFP-Puro
Alias: NM_018417.6;HCA2; HEL-S-7a; hsAC; SAC; SACI; Sacy
Gene lenght: 4830bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-4×Mito-ADCY10-3×Myc-CopGFP-Puro plasmid Description

pLV3-CMV-4×Mito-ADCY10-3×Myc-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-4×Mito-ADCY10-3×Myc-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc cttaagcttG CCACCATGAG CGTGCTGACC 2341 CCCCTGCTGC TGCGCGGCCT GACCGGCAGC GCCCGCCGCC TGCCCGTGCC CCGCGCCAAG 2401 ATCCACAGCC TGGGCGACCC CATGAGCGTG CTGACGCCAC TTCTCTTGCG CGGACTCACA 2461 GGATCAGCTA GGCGACTGCC CGTCCCTAGA GCCAAAATCC ACTCCCTGGG CAAGCTGATG 2521 AGCGTGCTGA CTCCACTGCT GCTGAGAGGA CTGACAGGAT CAGCTCGCAG ACTGCCTGTG 2581 CCTAGAGCCA AGATCCACTC CCTGGGCGAC CCAATGTCTG TGCTGACACC CCTGCTGCTG 2641 AGAGGGCTGA CAGGTTCCGC TAGAAGACTG CCTGTGCCTA GAGCTAAGAT CCACAGCCTG 2701 GGCAAGCTGG GATCCGCCAC CATGAACACT CCAAAAGAAG AATTCCAGGA CTGGCCCATA 2761 GTCAGAATAG CAGCTCATTT ACCAGACCTC ATTGTCTATG GACATTTCTC CCCAGAGCGA 2821 CCCTTTATGG ATTATTTTGA CGGAGTCCTG ATGTTTGTTG ATATTTCAGG TTTTACTGCA 2881 ATGACTGAGA AGTTCAGCAG TGCCATGTAC ATGGACAGAG GGGCTGAGCA GTTGGTGGAG 2941 ATCCTCAACT ACCACATAAG TGCAATAGTG GAGAAAGTGT TGATTTTTGG AGGAGACATC 3001 CTGAAATTTG CAGGTGATGC ACTGCTAGCC CTGTGGAGGG TGGAGCGAAA GCAGCTGAAA 3061 AACATTATCA CAGTGGTAAT TAAATGTAGC CTGGAGATCC ATGGATTGTT TGAGACCCAG 3121 GAGTGGGAAG AAGGCCTAGA CATCCGAGTC AAGATAGGAC TGGCTGCTGG CCACATCAGC 3181 ATGTTGGTCT TTGGAGATGA AACACACAGC CACTTTCTGG TGATTGGTCA GGCAGTGGAC 3241 GATGTGCGCC TTGCCCAGAA CATGGCTCAG ATGAATGATG TTATTCTGTC ACCAAACTGC 3301 TGGCAGCTCT GTGACCGGAG CATGATTGAA ATTGAGAGTG TTCCAGATCA GAGAGCAGTT 3361 AAGGTTAACT TCTTAAAACC ACCCCCCAAT TTTAATTTTG ATGAATTTTT CACAAAGTGT 3421 ACGACCTTCA TGCATTATTA TCCTTCTGGT GAGCACAAAA ACCTCCTGAG GCTTGCATGC 3481 ACGCTGAAGC CTGATCCTGA ACTGGAGATG TCCCTACAAA AGTATGTGAT GGAAAGCATT 3541 TTGAAGCAGA TTGATAACAA ACAGCTTCAG GGCTATTTAT CTGAGCTTCG CCCAGTGACG 3601 ATTGTGTTTG TGAACCTGAT GTTTGAAGAC CAAGACAAAG CAGAAGAGAT AGGCCCAGCC 3661 ATCCAGGATG CCTATATGCA CATCACTTCT GTCCTGAAGA TCTTCCAAGG CCAAATCAAT 3721 AAAGTCTTCA TGTTTGACAA GGGCTGCTCT TTCCTCTGTG TCTTTGGCTT CCCTGGGGAA 3781 AAGGTACCTG ACGAGCTCAC TCATGCTCTG GAATGTGCTA TGGATATATT TGACTTCTGC 3841 TCTCAAGTCC ACAAAATCCA AACTGTATCC ATCGGTGTTG CCAGTGGGAT TGTCTTCTGT 3901 GGGATCGTTG GACACACTGT GAGACACGAG TACACAGTCA TTGGTCAAAA AGTCAACTTA 3961 GCTGCCAGGA TGATGATGTA CTACCCAGGA ATTGTGACCT GCGACTCTGT CACCTACAAT 4021 GGGAGCAACC TACCAGCGTA CTTTTTTAAA GAGCTTCCAA AGAAAGTTAT GAAAGGTGTT 4081 GCAGATTCTG GACCATTGTA TCAGTATTGG GGCCGTACTG AGAAAGTCAT GTTTGGTATG 4141 GCGTGCCTCA TCTGCAACAG AAAGGAGGAT TACCCTTTGC TGGGACGTAA TAAAGAGATC 4201 AACTACTTCA TGTATACTAT GAAGAAATTT TTGATATCTA ACAGCAGCCA AGTCTTAATG 4261 TATGAGGGAT TACCAGGATA TGGAAAAAGC CAGATACTTA TGAAAATTGA GTACCTGGCC 4321 CAAGGTAAGA ATCACAGGAT TATTGCCATT TCATTGAATA AGATCAGCTT CCATCAAACT 4381 TTCTATACCA TCCAGATGTT CATGGCCAAT GTCCTAGGCC TAGACACTTG TAAACATTAT 4441 AAAGAACGAC AGACCAACCT TCGAAATAAA GTCATGACAC TGTTGGATGA AAAGTTCTAC 4501 TGTCTTCTTA ATGACATTTT CCATGTTCAG TTCCCTATTT CTCGGGAGAT TTCCAGGATG 4561 AGCACCTTGA AAAAGCAAAA ACAATTGGAA ATATTGTTTA TGAAGATCTT GAAGCTGATA 4621 GTGAAAGAGG AAAGGATTAT TTTTATCATT GATGAGGCCC AGTTTGTGGA TTCGACCTCC 4681 TGGAGATTTA TGGAGAAGCT TATCCGGACT CTTCCTATCT TCATCATTAT GTCCCTGTGT 4741 CCCTTCGTTA ACATTCCCTG TGCAGCTGCC AGGGCCGTAA TAAAGAACAG GAACACCACC 4801 TACATTGTCA TTGGTGCAGT ACAGCCTAAC GACATCTCCA ACAAGATCTG TCTTGACCTC 4861 AATGTGAGCT GCATCTCCAA AGAACTGGAC TCGTACCTGG GGGAGGGAAG CTGTGGGATT 4921 CCATTTTACT GTGAAGAATT GCTTAAAAAC CTGGAACATC ATGAGGTACT CGTTTTCCAA 4981 CAAACGGAGT CTGAGGAAAA GACAAATAGG ACCTGGAATA ACCTGTTCAA GTATTCCATT 5041 AAGCTAACAG AGAAGTTAAA CATGGTTACT CTCCATAGTG ATAAGGAAAG TGAAGAAGTC 5101 TGTCACCTCA CAAGTGGTGT CAGACTGAAA AACCTGTCAC CTCCAACGTC ATTAAAAGAA 5161 ATCTCTCTGA TCCAGCTGGA TAGCATGAGA CTTTCCCACC AAATGCTGGT GAGATGTGCT 5221 GCCATCATTG GCCTGACCTT CACCACTGAG TTGTTGTTTG AGATTCTCCC CTGTTGGAAT 5281 ATGAAGATGA TGATCAAGAC CCTGGCAACC CTAGTGGAAT CTAACATTTT TTATTGTTTC 5341 CGGAATGGCA AGGAGCTTCA AAAGGCCCTG AAACAGAATG ATCCCTCATT TGAGGTGCAC 5401 TATCGTTCCT TGTCTCTGAA GCCCAGTGAA GGGATGGATC ACGGTGAAGA GGAACAGCTT 5461 CGTGAACTGG AGAATGAGGT GATCGAGTGC CACAGGATTC GATTCTGTAA CCCTATGATG 5521 CAGAAAACAG CCTACGAGCT GTGGCTCAAG GACCAGAGAA AAGCCATGCA CTTGAAATGT 5581 GCCCGCTTTT TAGAAGAAGA TGCCCACAGA TGTGACCACT GCCGAGGCAG GGACTTCATT 5641 CCCTATCATC ACTTCACAGT GAATATTCGG CTCAACGCTT TAGACATGGA TGCCATTAAA 5701 AAGATGGCTA TGTCTCATGG ATTTAAAACT GAAGAAAAGC TTATCTTGTC CAACTCAGAG 5761 ATTCCTGAGA CATCTGCATT TTTTCCTGAA AATCGCAGTC CTGAAGAAAT AAGAGAAAAG 5821 ATCTTGAATT TCTTTGACCA CGTTTTAACA AAAATGAAGA CATCTGACGA AGACATTATC 5881 CCTCTGGAAT CTTGCCAGTG TGAAGAAATC CTAGAGATTG TCATCTTGCC TCTGGCCCAC 5941 CATTTTCTGG CTTTGGGAGA AAATGACAAA GCCTTATATT ACTTCTTAGA AATTGCATCT 6001 GCTTATCTCA TCTTTTGTGA TAACTACATG GCATACATGT ATTTGAATGA AGGACAGAAG 6061 TTGCTAAAAA CTCTCAAGAA GGACAAATCT TGGAGCCAGA CATTTGAGTC TGCCACCTTT 6121 TACAGCCTCA AAGGTGAGGT CTGTTTCAAT ATGGGCCAGA TAGTGCTTGC CAAGAAAATG 6181 CTGAGGAAGG CACTGAAGCT CCTCAACCGA ATCTTTCCTT ACAACTTAAT CTCCTTGTTT 6241 CTCCATATCC ATGTCGAGAA AAACAGACAC TTTCATTATG TGAATCGGCA GGCCCAAGAG 6301 AGCCCACCTC CAGGGAAGAA GAGGCTGGCA CAACTTTACC GGCAAACTGT CTGCCTTTCC 6361 TTGCTGTGGC GCATCTATAG CTACAGTTAT CTTTTTCACT GCAAGTATTA TGCCCACCTG 6421 GCAGTTATGA TGCAAATGAA TACTGCACTG GAAACTCAAA ATTGTTTCCA GATCATTAAG 6481 GCTTACCTAG ACTATTCGCT ATACCACCAC CTGGCTGGCT ACAAAGGTGT GTGGTTCAAA 6541 TATGAAGTCA TGGCCATGGA GCACATCTTC AACCTCCCCC TGAAAGGCGA GGGCATTGAA 6601 ATCGTGGCAT ACGTGGCTGA GACACTGGTC TTCAACAAGC TCATAATGGG ACACCTGGAT 6661 TTGGCCATTG AGTTAGGCTC CCGAGCCCTT CAGATGTGGG CACTGCTCCA GAATCCCAAC 6721 CGACATTATC AGTCCCTCTG CAGACTTAGC AGATGTCTCC TTCTGAACAG CAGATACCCG 6781 CAATTGATCC AGGTGCTGGG GCGGCTGTGG GAGCTTTCTG TAACACAGGA ACACATCTTC 6841 AGCAAGGCAT TTTTCTATTT TGTCTGCTTG GACATCCTGC TTTATTCTGG TTTTGTTTAT 6901 AGAACATTTG AAGAATGTTT GGAATTCATA CACCAATACG AAAACAACAG AATCCTCAAG 6961 TTCCACAGTG GACTCCTCCT GGGACTTTAT TCCTCTGTAG CTATCTGGTA TGCCAGACTT 7021 CAGGAATGGG ACAACTTTTA CAAATTTTCC AATAGAGCTA AAAATCTTTT GCCAAGAAGA 7081 ACCATGACAC TTACTTACTA TGACGGAATA TCTAGGTACA TGGAGGGGCA AGTTCTTCAC 7141 CTTCAAAAAC AAATCAAAGA ACAGTCAGAG AATGCCCAAG CCAGTGGGGA GGAGCTACTC 7201 AAGAACTTGG AGAATCTGGT GGCTCAAAAT ACCACTGGCC CTGTCTTTTG CCCAAGGCTC 7261 TACCACCTGA TGGCTTACGT CTGTATATTA ATGGGAGATG GGCAGAAATG TGGCCTCTTC 7321 CTGAACACAG CCTTGCGGCT CTCTGAAACA CAGGGGAATA TACTGGAGAA ATGCTGGCTG 7381 AACATGAACA AAGAATCATG GTACTCAACC TCTGAGTTAA AAGAAGACCA ATGGCTTCAG 7441 ACGATCTTGA GTCTCCCATC ATGGGAAAAA ATTGTAGCAG GCAGGGTAAA CATTCAGGAT 7501 CTTCAAAAAA ACAAATTCCT GATGAGAGCT AATACCGTGG ACAATCATTT CCTCGAGGAA 7561 CAAAAACTAA TATCAGAGGA AGATTTGGAA CAGAAACTAA TCAGTGAAGA AGATTTAGAG 7621 CAAAAGTTAA TTTCCGAGGA GGACTTAtga CTGCGATATC CAGCgcggcc gcGaaggatc 7681 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag 7741 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg 7801 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata 7861 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg 7921 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat 7981 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc 8041 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg 8101 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac 8161 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg 8221 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc 8281 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc 8341 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc 8401 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc 8461 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag 8521 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc 8581 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc 8641 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg 8701 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg 8761 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc 8821 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg 8881 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc 8941 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga 9001 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc 9061 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 9121 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 9181 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 9241 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 9301 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 9361 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 9421 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 9481 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 9541 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 9601 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac 9661 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 9721 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 9781 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 9841 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 9901 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 9961 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 10021 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg 10081 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 10141 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg 10201 agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag 10261 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 10321 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 10381 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 10441 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 10501 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 10561 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 10621 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 10681 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 10741 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 10801 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 10861 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat 10921 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 10981 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 11041 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 11101 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 11161 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 11221 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 11281 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 11341 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 11401 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 11461 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 11521 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 11581 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 11641 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 11701 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 11761 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 11821 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 11881 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 11941 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 12001 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 12061 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 12121 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 12181 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 12241 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 12301 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 12361 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 12421 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 12481 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 12541 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 12601 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 12661 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 12721 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 12781 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 12841 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 12901 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 12961 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 13021 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 13081 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 13141 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 13201 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 13261 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag 13321 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 13381 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 13441 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 13501 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.