pLV3-CMV-6×His-Ap2a2-EF1a-mCherry-Puro plasmid

PVT98464

pLV3-CMV-6×His-Ap2a2-EF1a-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-6×His-Ap2a2(mouse)-EF1a-mCherry-Puro
Alias: NM_007459.3;2410074K14Rik; Adtab; L25
Gene lenght: 2817bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-6×His-Ap2a2-EF1a-mCherry-Puro plasmid Description

pLV3-CMV-6×His-Ap2a2-EF1a-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-6×His-Ap2a2-EF1a-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgCATCA CCATCACCAT CAcatgccgg ccgtatccaa 2341 gggggacggg atgcgaggcc tagcggtctt catctccgac atccgcaact gtaaaagtaa 2401 agaagctgaa ataaagagaa taaacaaaga actggcaaat attagatcca aatttaaagg 2461 tgacaaggct cttgatggct acagtaaaaa gaagtatgtc tgcaaattgc tcttcatctt 2521 tctccttggt catgacattg actttggaca catggaagct gtgaatcttc tgagctcaaa 2581 cagatacacg gaaaagcaga ttggctacct tttcatctct gtattggtga actcgaatag 2641 tgaactgatc cggttgatta acaatgccat caagaatgac ctggccagcc gcaatcctac 2701 gtttatgggt ctcgccctgc actgcattgc caatgtgggt agccgtgaga tggcggaggc 2761 cttcgccgga gagatcccca agatcctcgt ggcaggagat accatggata gtgtgaagca 2821 gagcgcagcc ctgtgcttgt tacgcctgta caggacatca cctgacctag ttcccatggg 2881 cgactggaca tctcgtgtgg tacacctcct caatgaccag cacttggggg tagtgactgc 2941 agcgactagc ctcatcacca ccctggccca gaagaaccct gaggagttca agacctctgt 3001 ctcgctagct gtctcgcgac tgagcaggat tgtgacgtct gcatcaacag atcttcagga 3061 ttatacttac tactttgtcc cggctccttg gctgtcagtc aagcttctga gactgctgca 3121 gtgctaccca cccccagacc ctgcggtgcg tggccgcctg actgagtgct tggagaccat 3181 cctgaacaag gcccaggagc cacccaagtc caagaaggtc cagcattcaa atgccaagaa 3241 cgctgtgctg tttgaggcca tcagcctgat catccaccat gacagtgagc caaacttgct 3301 cgtccgcgcc tgcaaccagc tgggccagtt cctgcagcac cgggagacga acctacgcta 3361 cctggctctg gagagcatgt gcacgctggc cagctctgag ttctcccacg aggccgtcaa 3421 gacccacatt gagacggtca tcaatgcctt gaagacggaa agagacgtga gtgtgaggca 3481 gcgggcagtg gacctgctct acgccatgtg tgaccggagc aacgcccagc agattgtggc 3541 tgagatgctg agctacctag aaacggctga ctactccatc cgagaggaaa ttgtgctgaa 3601 ggtggccatc ctggctgaga agtatgcagt ggactacact tggtatgtgg acaccatcct 3661 caacctcatc cgcatcgcgg gcgactatgt gagcgaggag gtgtggtacc gcgtcatcca 3721 gattgtcatc aatcgcgatg atgtgcaggg ctatgctgcc aagacggtat ttgaggcatt 3781 gcaggcgcca gcatgtcatg agaacttggt caaagtgggc ggctacatcc tgggggagtt 3841 tggaaacttg atagctgggg acccacgatc cagccctctg atccagttca acctgctcca 3901 ctccaagttc cacttgtgca gcgtccccac tcgggcactc ctgctgtcca cctacatcaa 3961 gttcgtgaac ctctttccag aggtaaaagc taccattcag gacgtgctgc gcagtgacag 4021 ccagctgaag aatgccgacg tggagctgca gcagcgggct gtggagtacc tgcgactgag 4081 cactgtcgcc agcactgaca tcctggcaac tgtcctggag gaaatgccac ccttccctga 4141 gcgtgagtcc tccatcttgg ccaagctgaa gaagaagaag ggcccaagca cagtgactga 4201 cctggaggag accaagcggg agcgaagcat cgatgtgaat gggggccctg aacccgttcc 4261 agccagcacc agtgctgcgt ccacaccttc tccgtcagca gacctgctgg gtctgggggc 4321 cgtgccccct gctcccacgg ggccccctcc ctcctctggc ggcgggctgc tggtggacgt 4381 gttctcagac tcagcttctg cagtcgcacc tcttgcaccc ggctctgaag acaactttgc 4441 caggtttgtt tgtaaaaata atggtgtatt gttcgaaaac cagctgcttc aaattggact 4501 taagtcagaa tttcggcaga atttaggccg aatgttcata ttttatggta acaagacctc 4561 cacgcagttc ctaaacttca ctccaacact aatctgtgct gacgaccttc aaactaatct 4621 gaacctgcag actaagcccg tggacccaac tgtggatggg ggcgcacagg tgcaacaagt 4681 ggtcaacatt gagtgtatat ctgatttcac agaggcacct gtcctcaaca tccagttcag 4741 gtatggtggg accttccaga atgtgtctgt taagcttccc atcactctca acaaattttt 4801 ccagcccaca gaaatggctt ctcaggattt ctttcaacgt tggaagcagt tgagcaatcc 4861 acagcaagaa gtgcagaata tcttcaaagc aaagcatccg atggacacag agatcactaa 4921 ggcaaagatt attggatttg gttctgcgct cctggaagaa gttgacccga atcctgcaaa 4981 tttcgtgggt gctggcataa tacacaccaa aaccacccag attggctgct tgttgcgcct 5041 ggagccaaac ctgcaagctc agatgtatag actcaccttg cgtaccagca aagacaccgt 5101 ctctcagaga ctatgtgaat tgctgtcgga acagttctag ggatccgcgg ccgcGaagga 5161 tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga 5221 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact 5281 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata 5341 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc 5401 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc 5461 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt 5521 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt 5581 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct 5641 acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta 5701 cgctagacgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt 5761 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg 5821 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg 5881 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg 5941 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct 6001 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact 6061 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct 6121 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt 6181 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg 6241 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg 6301 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg 6361 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca 6421 agggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg 6481 gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg 6541 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc 6601 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 6661 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 6721 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 6781 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 6841 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 6901 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 6961 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 7021 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg 7081 cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 7141 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 7201 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 7261 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 7321 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 7381 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 7441 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 7501 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 7561 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 7621 gtctccgcct tcgccctcag acgagtcgga tctccctttg gccgcctccc cgcctggtac 7681 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 7741 ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg 7801 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 7861 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 7921 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 7981 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 8041 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 8101 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 8161 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc 8221 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 8281 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag 8341 agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 8401 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 8461 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 8521 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 8581 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 8641 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 8701 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 8761 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 8821 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 8881 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 8941 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 9001 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 9061 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 9121 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 9181 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 9241 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 9301 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 9361 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 9421 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 9481 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 9541 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 9601 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 9661 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 9721 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 9781 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 9841 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 9901 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 9961 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 10021 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 10081 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 10141 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 10201 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 10261 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 10321 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 10381 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 10441 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 10501 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 10561 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 10621 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 10681 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 10741 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 10801 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 10861 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 10921 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 10981 tgccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???