pLV3-CMV-ACLY-3×HA-CopGFP-Neo plasmid

PVT94917

pLV3-CMV-ACLY-3×HA-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-ACLY(human)-3×HA-CopGFP-Neo
Alias: NM_001096.3;ACL; ATPCL; CLATP
Gene lenght: 3303bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ACLY-3×HA-CopGFP-Neo plasmid Description

pLV3-CMV-ACLY-3×HA-CopGFP-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-ACLY-3×HA-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgtcggc caaggcaatt tcagagcaga cgggcaaaga 2341 actcctttac aagttcatct gtaccacctc agccatccag aatcggttca agtatgctcg 2401 ggtcactcct gacacagact gggcccgctt gctgcaggac cacccctggc tgctcagcca 2461 gaacttggta gtcaagccag accagctgat caaacgtcgt ggaaaacttg gtctcgttgg 2521 ggtcaacctc actctggatg gggtcaagtc ctggctgaag ccacggctgg gacaggaagc 2581 cacagttggc aaggccacag gcttcctcaa gaactttctg atcgagccct tcgtccccca 2641 cagtcaggct gaggagttct atgtctgcat ctatgccacc cgagaagggg actacgtcct 2701 gttccaccac gaggggggtg tggacgtggg tgatgtggac gccaaggccc agaagctgct 2761 tgttggcgtg gatgagaaac tgaatcctga ggacatcaaa aaacacctgt tggtccacgc 2821 ccctgaagac aagaaagaaa ttctggccag ttttatctcc ggcctcttca atttctacga 2881 ggacttgtac ttcacctacc tcgagatcaa tccccttgta gtgaccaaag atggagtcta 2941 tgtccttgac ttggcggcca aggtggacgc cactgccgac tacatctgca aagtgaagtg 3001 gggtgacatc gagttccctc cccccttcgg gcgggaggca tatccagagg aagcctacat 3061 tgcagacctc gatgccaaaa gtggggcaag cctgaagctg accttgctga accccaaagg 3121 gaggatctgg accatggtgg ccgggggtgg cgcctctgtc gtgtacagcg ataccatctg 3181 tgatctaggg ggtgtcaacg agctggcaaa ctatggggag tactcaggcg cccccagcga 3241 gcagcagacc tatgactatg ccaagactat cctctccctc atgacccgag agaagcaccc 3301 agatggcaag atcctcatca ttggaggcag catcgcaaac ttcaccaacg tggctgccac 3361 gttcaagggc atcgtgagag caattcgaga ttaccagggc cccctgaagg agcacgaagt 3421 cacaatcttt gtccgaagag gtggccccaa ctatcaggag ggcttacggg tgatgggaga 3481 agtcgggaag accactggga tccccatcca tgtctttggc acagagactc acatgacggc 3541 cattgtgggc atggccctgg gccaccggcc catccccaac cagccaccca cagcggccca 3601 cactgcaaac ttcctcctca acgccagcgg gagcacatcg acgccagccc ccagcaggac 3661 agcatctttt tctgagtcca gggccgatga ggtggcgcct gcaaagaagg ccaagcctgc 3721 catgccacaa gattcagtcc caagtccaag atccctgcaa ggaaagagca ccaccctctt 3781 cagccgccac accaaggcca ttgtgtgggg catgcagacc cgggccgtgc aaggcatgct 3841 ggactttgac tatgtctgct cccgagacga gccctcagtg gctgccatgg tctacccttt 3901 cactggggac cacaagcaga agttttactg ggggcacaaa gagatcctga tccctgtctt 3961 caagaacatg gctgatgcca tgaggaagca tccggaggta gatgtgctca tcaactttgc 4021 ctctctccgc tctgcctatg acagcaccat ggagaccatg aactatgccc agatccggac 4081 catcgccatc atagctgaag gcatccctga ggccctcacg agaaagctga tcaagaaggc 4141 ggaccagaag ggagtgacca tcatcggacc tgccactgtt ggaggcatca agcctgggtg 4201 ctttaagatt ggcaacacag gtgggatgct ggacaacatc ctggcctcca aactgtaccg 4261 cccaggcagc gtggcctatg tctcacgttc cggaggcatg tccaacgagc tcaacaatat 4321 catctctcgg accacggatg gcgtctatga gggcgtggcc attggtgggg acaggtaccc 4381 gggctccaca ttcatggatc atgtgttacg ctatcaggac actccaggag tcaaaatgat 4441 tgtggttctt ggagagattg ggggcactga ggaatataag atttgccggg gcatcaagga 4501 gggccgcctc actaagccca tcgtctgctg gtgcatcggg acgtgtgcca ccatgttctc 4561 ctctgaggtc cagtttggcc atgctggagc ttgtgccaac caggcttctg aaactgcagt 4621 agccaagaac caggctttga aggaagcagg agtgtttgtg ccccggagct ttgatgagct 4681 tggagagatc atccagtctg tatacgaaga tctcgtggcc aatggagtca ttgtacctgc 4741 ccaggaggtg ccgcccccaa ccgtgcccat ggactactcc tgggccaggg agcttggttt 4801 gatccgcaaa cctgcctcgt tcatgaccag catctgcgat gagcgaggac aggagctcat 4861 ctacgcgggc atgcccatca ctgaggtctt caaggaagag atgggcattg gcggggtcct 4921 cggcctcctc tggttccaga aaaggttgcc taagtactct tgccagttca ttgagatgtg 4981 tctgatggtg acagctgatc acgggccagc cgtctctgga gcccacaaca ccatcatttg 5041 tgcgcgagct gggaaagacc tggtctccag cctcacctcg gggctgctca ccatcgggga 5101 tcggtttggg ggtgccttgg atgcagcagc caagatgttc agtaaagcct ttgacagtgg 5161 cattatcccc atggagtttg tgaacaagat gaagaaggaa gggaagctga tcatgggcat 5221 tggtcaccga gtgaagtcga taaacaaccc agacatgcga gtgcagatcc tcaaagatta 5281 cgtcaggcag cacttccctg ccactcctct gctcgattat gcactggaag tagagaagat 5341 taccacctcg aagaagccaa atcttatcct gaatgtagat ggtctcatcg gagtcgcatt 5401 tgtagacatg cttagaaact gtgggtcctt tactcgggag gaagctgatg aatatattga 5461 cattggagcc ctcaatggca tctttgtgct gggaaggagt atggggttca ttggacacta 5521 tcttgatcag aagaggctga agcaggggct gtatcgtcat ccgtgggatg atatttcata 5581 tgttcttccg gaacacatga gcatgTACCC ATACGACGTA CCTGATTATG CAACTTATCC 5641 TTATGATGTA CCTGATTATG CTAGCTATCC TTATGATGTC CCAGACTACG CATAgcggcc 5701 gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 5761 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg 5821 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 5881 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 5941 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg 6001 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg 6061 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc 6121 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc 6181 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc 6241 ggcgcctacg ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat 6301 cgagtgccgc atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga 6361 gggcaccccc aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac 6421 cttcagcccc tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta 6481 ccccagcggc tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac 6541 ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga 6601 ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt 6661 gatcttcacc gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg 6721 cgataacgtg ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta 6781 cagcttcgtg gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca 6841 gaacgggggc cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct 6901 gggcatcgtg gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc 6961 tcagtcgtcc aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga 7021 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctGCCACCat 7081 ggctgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 7141 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 7201 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 7261 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 7321 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 7381 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 7441 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 7501 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 7561 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 7621 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 7681 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 7741 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 7801 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 7861 cgagttcttc tgaGTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG 7921 TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA 7981 TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT 8041 GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT 8101 TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC 8161 TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG 8221 CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC 8281 GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG 8341 CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT 8401 GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC 8461 CTCCCCGCgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 8521 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata agatctgctt 8581 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 8641 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 8701 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 8761 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 8821 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 8881 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 8941 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 9001 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 9061 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 9121 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa 9181 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 9241 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 9301 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 9361 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 9421 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 9481 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 9541 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 9601 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 9661 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 9721 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 9781 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 9841 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 9901 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 9961 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 10021 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 10081 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 10141 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 10201 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 10261 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 10321 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 10381 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 10441 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 10501 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 10561 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 10621 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 10681 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 10741 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 10801 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 10861 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 10921 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 10981 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 11041 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 11101 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 11161 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 11221 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 11281 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 11341 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 11401 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 11461 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 11521 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 11581 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca 11641 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 11701 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 11761 aaacgacggc cagtgccaag ctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???