pLV3-CMV-Asic1-3×FLAG-CopGFP-Puro plasmid

PVT107337

pLV3-CMV-Asic1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Asic1(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_009597.2;Accn2; ASIC; ASIC1a; ASIC1b; B530003N02Rik; BNaC2
Gene lenght: 1578
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Asic1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Asic1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-Asic1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg aactgaagac cgaggaggag 2341 gaggtgggtg gtgtccagcc ggtgagcatc caggcttttg ccagcagctc cacgctgcac 2401 ggtcttgccc acatcttctc ctatgagcgg ctgtctctga agcgggcact gtgggccctg 2461 tgtttcctgg gttcgctggc cgtcctgctg tgtgtgtgca ctgagcgtgt gcagtactac 2521 ttctgctacc accacgtcac caagctcgac gaggtggctg cctcccagct caccttccct 2581 gccgtcactc tctgcaacct caatgagttt cgctttagcc aagtctccaa gaatgacctg 2641 taccatgctg gggaactgct ggccctgctc aacaacaggt atgagatacc ggacacacag 2701 atggctgatg aaaagcagct ggagatattg caggacaagg ccaacttccg tagcttcaag 2761 cccaagccct tcaacatgcg tgagttctac gacagagcag ggcatgacat tcgagacatg 2821 cttctctcgt gccacttccg aggggaggcc tgcagcgctg aagacttcaa agtggtcttc 2881 acgcggtatg ggaagtgcta cacattcaac tcgggccaag atgggcggcc acggctgaag 2941 accatgaaag gtgggactgg caacggcctg gagatcatgc tggacattca gcaagatgaa 3001 tacttgcctg tgtggggaga gactgatgag acatcgttcg aagcaggcat caaagtgcag 3061 atccacagtc aggacgagcc tcctttcatc gaccagctgg gctttggcgt ggccccaggc 3121 ttccagacgt ttgtgtcttg ccaggagcag aggctcatct acctgccctc cccctggggc 3181 acctgcaatg ctgttaccat ggactcggat ttcttcgact cctacagcat cacggcctgc 3241 cggattgatt gtgaaacccg ttacctggtg gaaaactgca actgccgtat ggtgcacatg 3301 ccaggggatg ccccatactg tactccggag cagtacaagg agtgtgcaga ccctgccctg 3361 gacttcctag tggagaaaga ccaggaatac tgtgtgtgtg agatgccctg caacctgacc 3421 cgctacggca aggagctgtc catggtcaag atccccagca aagcctcagc caagtacctg 3481 gccaagaagt tcaacaaatc tgaacagtac ataggggaga atattctggt gctggacatt 3541 ttctttgaag tcctcaacta tgagaccatc gagcagaaga aggcctatga gatcgcaggg 3601 cttttgggtg acatcggggg ccagatggga ttgttcatcg gggccagcat cctcacagtg 3661 ctggaactct ttgactatgc ctatgaggtc attaagcacc ggctgtgtag acgtgggaag 3721 tgccagaagg aggctaagag gaacagcgca gataagggcg tggcgctcag cctggatgac 3781 gtcaaaagac acaatccctg cgagagcctc cgaggacatc ctgccgggat gacgtacgct 3841 gccaacatcc tacctcacca tcccgctcga ggcacgtttg aggactttac ctgcggatcc 3901 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT 3961 GACAAGtagc ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc 4021 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag 4081 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 4141 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 4201 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc 4261 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc 4321 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg 4381 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc 4441 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga 4501 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg 4561 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg 4621 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc 4681 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac 4741 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc 4801 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc 4861 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc 4921 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac 4981 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc 5041 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac 5101 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac 5161 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc 5221 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc 5281 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 5341 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 5401 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 5461 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 5521 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 5581 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 5641 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 5701 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 5761 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 5821 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 5881 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 5941 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 6001 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 6061 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 6121 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 6181 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 6241 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 6301 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aATCATCGTC CTTTCCTtgg 6361 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 6421 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 6481 cgtctTcgcc ttcgccctca gacgagtcgg atctcccttt ggGccgcctc cccgcctggt 6541 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 6601 ggggggactg gaagggctaa ttcactccca acgaaGataa gatctgcttt ttgcttgtac 6661 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 6721 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 6781 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 6841 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 6901 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 6961 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 7021 catcaatgta tcttatcatg tctggctcta gctaTCCCGC CCCTAACTCC GCCCAtcccg 7081 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 7141 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 7201 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt 7261 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 7321 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 7381 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 7441 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 7501 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 7561 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 7621 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7681 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7741 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7801 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7861 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7921 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7981 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 8041 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 8101 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 8161 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 8221 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 8281 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 8341 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 8401 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 8461 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 8521 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 8581 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 8641 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8701 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 8761 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8821 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8881 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8941 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 9001 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 9061 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 9121 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 9181 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 9241 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 9301 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 9361 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 9421 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 9481 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 9541 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 9601 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 9661 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 9721 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 9781 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 9841 ttgtaaaacg acggccagtg ccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.