pLV3-CMV-BIRC2-BIRC3-3×FLAG-CopGFP-Puro plasmid

PVT109171

pLV3-CMV-BIRC2-BIRC3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-BIRC2(human)-BIRC3(human)-3×FLAG-CopGFP-Puro
Alias: NM_001166.5;API1; c-IAP1; cIAP1; Hiap-2; HIAP2; MIHB;RNF48;AIP1; API2; c-IAP2; CIAP2; HAIP1; HIAP1;IAP-1; MALT2; MIHC; RNF49
Gene lenght: 3666
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-BIRC2-BIRC3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-BIRC2-BIRC3-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-BIRC2-BIRC3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgc acaaaactgc ctcccaaaga 2341 cttttcccag gtccctcgta tcaaaacatt aagagtataa tggaagatag cacgatcttg 2401 tcagattgga caaacagcaa caaacaaaaa atgaagtatg acttttcctg tgaactctac 2461 agaatgtcta catattcaac tttccccgcc ggggtgcctg tctcagaaag gagtcttgct 2521 cgtgctggtt tttattatac tggtgtgaat gacaaggtca aatgcttctg ttgtggcctg 2581 atgctggata actggaaact aggagacagt cctattcaaa agcataaaca gctatatcct 2641 agctgtagct ttattcagaa tctggtttca gctagtctgg gatccacctc taagaatacg 2701 tctccaatga gaaacagttt tgcacattca ttatctccca ccttggaaca tagtagcttg 2761 ttcagtggtt cttactccag cctttctcca aaccctctta attctagagc agttgaagac 2821 atctcttcat cgaggactaa cccctacagt tatgcaatga gtactgaaga agccagattt 2881 cttacctacc atatgtggcc attaactttt ttgtcaccat cagaattggc aagagctggt 2941 ttttattata taggacctgg agatagggta gcctgctttg cctgtggtgg gaagctcagt 3001 aactgggaac caaaggatga tgctatgtca gaacaccgga ggcattttcc caactgtcca 3061 tttttggaaa attctctaga aactctgagg tttagcattt caaatctgag catgcagaca 3121 catgcagctc gaatgagaac atttatgtac tggccatcta gtgttccagt tcagcctgag 3181 cagcttgcaa gtgctggttt ttattatgtg ggtcgcaatg atgatgtcaa atgcttttgt 3241 tgtgatggtg gcttgaggtg ttgggaatct ggagatgatc catgggtaga acatgccaag 3301 tggtttccaa ggtgtgagtt cttgatacga atgaaaggcc aagagtttgt tgatgagatt 3361 caaggtagat atcctcatct tcttgaacag ctgttgtcaa cttcagatac cactggagaa 3421 gaaaatgctg acccaccaat tattcatttt ggacctggag aaagttcttc agaagatgct 3481 gtcatgatga atacacctgt ggttaaatct gccttggaaa tgggctttaa tagagacctg 3541 gtgaaacaaa cagttcaaag taaaatcctg acaactggag agaactataa aacagttaat 3601 gatattgtgt cagcacttct taatgctgaa gatgaaaaaa gagaagagga gaaggaaaaa 3661 caagctgaag aaatggcatc agatgatttg tcattaattc ggaagaacag aatggctctc 3721 tttcaacaat tgacatgtgt gcttcctatc ctggataatc ttttaaaggc caatgtaatt 3781 aataaacagg aacatgatat tattaaacaa aaaacacaga tacctttaca agcgagagaa 3841 ctgattgata ccattttggt taaaggaaat gctgcggcca acatcttcaa aaactgtcta 3901 aaagaaattg actctacatt gtataagaac ttatttgtgg ataagaatat gaagtatatt 3961 ccaacagaag atgtttcagg tctgtcactg gaagaacaat tgaggaggtt gcaagaagaa 4021 cgaacttgta aagtgtgtat ggacaaagaa gtttctgttg tatttattcc ttgtggtcat 4081 ctggtagtat gccaggaatg tgccccttct ctaagaaaat gccctatttg caggggtata 4141 atcaagggta ctgttcgtac atttctctct ggtggaggcg gatcaggagg cggtggatct 4201 atgaacatag tagaaaacag catattctta tcaaatttga tgaaaagcgc caacacgttt 4261 gaactgaaat acgacttgtc atgtgaactg taccgaatgt ctacgtattc cacttttcct 4321 gctggggttc ctgtctcaga aaggagtctt gctcgtgctg gtttctatta cactggtgtg 4381 aatgacaagg tcaaatgctt ctgttgtggc ctgatgctgg ataactggaa aagaggagac 4441 agtcctactg aaaagcataa aaagttgtat cctagctgca gattcgttca gagtctaaat 4501 tccgttaaca acttggaagc tacctctcag cctacttttc cttcttcagt aacaaattcc 4561 acacactcat tacttccggg tacagaaaac agtggatatt tccgtggctc ttattcaaac 4621 tctccatcaa atcctgtaaa ctccagagca aatcaagatt tttctgcctt gatgagaagt 4681 tcctaccact gtgcaatgaa taacgaaaat gccagattac ttacttttca gacatggcca 4741 ttgacttttc tgtcgccaac agatctggca aaagcaggct tttactacat aggacctgga 4801 gacagagtgg cttgctttgc ctgtggtgga aaattgagca attgggaacc gaaggataat 4861 gctatgtcag aacacctgag acattttccc aaatgcccat ttatagaaaa tcagcttcaa 4921 gacacttcaa gatacacagt ttctaatctg agcatgcaga cacatgcagc ccgctttaaa 4981 acattcttta actggccctc tagtgttcta gttaatcctg agcagcttgc aagtgcgggt 5041 ttttattatg tgggtaacag tgatgatgtc aaatgctttt gctgtgatgg tggactcagg 5101 tgttgggaat ctggagatga tccatgggtt caacatgcca agtggtttcc aaggtgtgag 5161 tacttgataa gaattaaagg acaggagttc atccgtcaag ttcaagccag ttaccctcat 5221 ctacttgaac agctgctatc cacatcagac agcccaggag atgaaaatgc agagtcatca 5281 attatccatt ttgaacctgg agaagaccat tcagaagatg caatcatgat gaatactcct 5341 gtgattaatg ctgccgtgga aatgggcttt agtagaagcc tggtaaaaca gacagttcag 5401 agaaaaatcc tagcaactgg agagaattat agactagtca atgatcttgt gttagactta 5461 ctcaatgcag aagatgaaat aagggaagag gagagagaaa gagcaactga ggaaaaagaa 5521 tcaaatgatt tattattaat ccggaagaat agaatggcac tttttcaaca tttgacttgt 5581 gtaattccaa tcctggatag tctactaact gccggaatta ttaatgaaca agaacatgat 5641 gttattaaac agaagacaca gacgtcttta caagcaagag aactgattga tacgatttta 5701 gtaaaaggaa atattgcagc cactgtattc agaaactctc tgcaagaagc tgaagctgtg 5761 ttatatgagc atttatttgt gcaacaggac ataaaatata ttcccacaga agatgtttca 5821 gatctaccag tggaagaaca attgcggaga ctacaagaag aaagaacatg taaagtgtgt 5881 atggacaaag aagtgtccat agtgtttatt ccttgtggtc atctagtagt atgcaaagat 5941 tgtgctcctt ctttaagaaa gtgtcctatt tgtaggagta caatcaaggg tacagttcgt 6001 acatttcttt caggatccGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC 6061 GACTACAAGG ATGACGATGA CAAGtagcgg ccgcGaagga tctgcgatcg ctccggtgcc 6121 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 6181 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 6241 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 6301 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 6361 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 6421 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 6481 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 6541 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 6601 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag 6661 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac 6721 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc 6781 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg 6841 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg 6901 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc 6961 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag 7021 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc 7081 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc 7141 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac 7201 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc 7261 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc 7321 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc 7381 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt 7441 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc 7501 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 7561 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 7621 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 7681 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 7741 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 7801 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 7861 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 7921 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 7981 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 8041 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg 8101 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 8161 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 8221 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 8281 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 8341 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 8401 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaA 8461 TCATCGTCCT TTCCTtggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 8521 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 8581 gctctgcggc ctcttccgcg tctTcgcctt cgccctcaga cgagtcggat ctccctttgg 8641 Gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 8701 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaGataaga 8761 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 8821 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 8881 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 8941 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 9001 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta 9061 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 9121 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc taTCCCGCCC 9181 CTAACTCCGC CCAtcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 9241 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 9301 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat 9361 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 9421 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 9481 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 9541 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 9601 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 9661 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 9721 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 9781 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 9841 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 9901 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 9961 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 10021 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 10081 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 10141 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 10201 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 10261 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 10321 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 10381 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 10441 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 10501 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 10561 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 10621 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 10681 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 10741 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 10801 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 10861 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 10921 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 10981 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 11041 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 11101 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 11161 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 11221 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 11281 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 11341 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 11401 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 11461 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 11521 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 11581 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 11641 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 11701 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 11761 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 11821 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 11881 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 11941 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.