pLV3-CMV-BMP6-EF1a-mCherry-Puro plasmid

PVT109925

pLV3-CMV-BMP6-EF1a-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-BMP6(human-gc-opt)-EF1a-mCherry-Puro
Alias: NM_001718.6;IO; VGR; VGR1
Gene lenght: 1539
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-BMP6-EF1a-mCherry-Puro plasmid Description

pLV3-CMV-BMP6-EF1a-mCherry-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-BMP6-EF1a-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccATGCCTGG ACTGGGAAGG AGAGCCCAGT GGCTGTGTTG 2341 GTGGTGGGGA CTGCTGTGTT CCTGCTGCGG ACCTCCTCCA CTGAGGCCTC CTCTGCCTGC 2401 TGCTGCTGCT GCCGCTGCTG GAGGACAGCT GCTGGGAGAC GGAGGATCTC CAGGAAGAAC 2461 CGAGCAGCCT CCTCCCTCTC CACAGTCTAG CTCCGGATTC CTGTACAGAA GGCTGAAGAC 2521 ACAGGAGAAG AGAGAGATGC AGAAGGAGAT CCTGTCCGTG CTGGGCCTGC CTCATAGACC 2581 AAGACCCCTG CACGGCCTGC AGCAACCTCA ACCTCCTGCT CTGAGGCAGC AGGAGGAGCA 2641 ACAGCAGCAG CAGCAACTGC CCAGGGGAGA ACCACCTCCA GGAAGACTGA AGAGCGCCCC 2701 ACTGTTTATG CTGGATCTGT ACAATGCCCT GAGCGCCGAT AATGACGAGG ATGGCGCCTC 2761 CGAGGGCGAA AGGCAGCAGA GCTGGCCTCA TGAGGCCGCT TCTAGCAGCC AGAGAAGGCA 2821 GCCTCCTCCT GGAGCTGCTC ACCCTCTGAA CAGAAAGTCC CTGCTGGCCC CCGGAAGCGG 2881 AAGCGGAGGA GCTAGCCCTC TGACATCCGC TCAGGACAGC GCTTTTCTGA ATGATGCCGA 2941 TATGGTGATG TCCTTCGTGA ACCTGGTGGA GTACGATAAG GAGTTCTCCC CCAGACAGAG 3001 ACACCACAAG GAGTTTAAGT TCAACCTGAG CCAGATCCCT GAGGGCGAGG TGGTTACAGC 3061 CGCTgaattc cgcatctaca aggactgtgt tatggggagt tttaaaaacc aaacttttct 3121 tatcagcatt tatcaagtct tacaggagca tcagcacaga gactctgacc tgtttttgtt 3181 ggacacccgt gtagtatggg cctcagaaga aggctggctg gaatttgaca tcacggccac 3241 tagcaatctg tgggttgtga ctccacagca taacatgggg cttcagctga gcgtggtgac 3301 aagggatgga gtccacgtcc acccccgagc cgcaggcctg gtgggcagag acggccctta 3361 cgacaagcag cccttcatgg tggctttctt caaagtgagt gaggtgcacg tgcgcaccac 3421 caggtcagcc tccagccggc gccgacaaca gagtcgtaat cgctctaccc agtcccagga 3481 cgtggcgcgg gtctccagtg cttcagatta caacagcagt gaattgaaaa cagcctgcag 3541 gaagcatgag ctgtatgtga gtttccaaga cctgggatgg caggactgga tcattgcacc 3601 caagggctat gctgccaatt actgtgatgg agaatgctcc ttcccactca acgcacacat 3661 gaatgcaacc aaccacgcga ttgtgcagac cttggttcac cttatgaacc ccgagtatgt 3721 ccccaaaccg tgctgtgcgc caactaagct aaatgccatc tcggttcttt actttgatga 3781 caactccaat gtcattctga aaaaatacag gaatatggtt gtaagagctt gtggatgcca 3841 ctaaggatcc gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag tgggcagagc 3901 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct 3961 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 4021 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca 4081 acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg 4141 cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg 4201 cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac 4261 cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt 4321 gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca 4381 gatccaagct gtgaccggcg cctacgctag acgccaccat ggtgagcaag ggcgaggagg 4441 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga 4501 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga 4561 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc 4621 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact 4681 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 4741 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga 4801 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct 4861 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc 4921 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg 4981 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct 5041 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca 5101 ccggcggcat ggacgagctg tacaagggat ctcgcgaggg cagaggaagt cttctaacat 5161 gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg gtgcgcctcg 5221 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc 5281 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag 5341 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg 5401 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga 5461 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag 5521 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct 5581 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg 5641 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct 5701 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct 5761 ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa 5821 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 5881 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 5941 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 6001 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 6061 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 6121 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 6181 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 6241 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 6301 tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc 6361 tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc 6421 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat 6481 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc 6541 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 6601 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 6661 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat 6721 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg 6781 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 6841 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc 6901 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 6961 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 7021 ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt 7081 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 7141 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 7201 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 7261 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 7321 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 7381 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 7441 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 7501 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 7561 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 7621 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 7681 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 7741 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 7801 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 7861 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 7921 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7981 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 8041 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 8101 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 8161 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 8221 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 8281 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 8341 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 8401 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 8461 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 8521 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 8581 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 8641 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 8701 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 8761 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 8821 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 8881 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 8941 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 9001 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 9061 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 9121 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 9181 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 9241 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 9301 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 9361 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 9421 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 9481 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 9541 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 9601 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 9661 acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.