pLV3-CMV-CELSR3-3×FLAG-T2A-EGFP-Puro plasmid

PVT107847

pLV3-CMV-CELSR3-3×FLAG-T2A-EGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-CELSR3(human)-3×FLAG-T2A-EGFP-Puro
Alias: NM_001407.3;ADGRC3; CDHF11; EGFL1; FMI1; HFMI1; MEGF2;RESDA1
Gene lenght: 9936
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CELSR3-3×FLAG-T2A-EGFP-Puro plasmid Description

pLV3-CMV-CELSR3-3×FLAG-T2A-EGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-CELSR3-3×FLAG-T2A-EGFP-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catgatggcg aggcggccgc cgtggcgggg cctcggggga 2341 cggtcgaccc ccatactcct gctccttctc ctctctttgt tccccctcag ccaggaggag 2401 ctggggggcg gtgggcacca gggctgggac ccaggcttag ctgccactac ggggccaagg 2461 gcgcatatcg gtggcggagc cttagctctt tgtccggagt cttccggggt ccgggaggat 2521 ggggggcctg gcctgggggt cagggagcct atcttcgtgg ggctccgagg gagaaggcaa 2581 agcgcccgga atagtcgagg gccccctgag cagccgaatg aggagctggg gattgaacac 2641 ggcgtccagc cattgggcag ccgcgaacga gagacaggac agggaccagg gtctgtgtta 2701 tactggcgcc cagaggtctc ctcttgcggg cggacaggac ctttgcaaag aggtagtctg 2761 tcaccagggg ctctgtcctc aggggtcccg ggctcgggga acagctcgcc cctcccttca 2821 gactttttga ttcggcacca cggtcccaag ccggtgtcct cccagcggaa cgctgggaca 2881 ggctcccgca aaagagtggg caccgcgcgc tgctgtgggg aattatgggc aacagggagc 2941 aagggtcagg gcgagagagc cacgacatcc ggagcagaaa ggacagcccc ccggcggaac 3001 tgtcttccag gggcctcggg atctggcccc gagctggatt cagcaccacg cacggcgagg 3061 acagctcctg catcaggttc agcaccccgc gagtctcgga cagctcccga gccggcgccc 3121 aagcgcatgc gctcccgggg tctcttccgc tgccgcttcc tcccgcagcg ccccgggccg 3181 cgtcccccgg gactcccggc ccgtcctgaa gccaggaaag taacctcggc gaaccgggca 3241 cgctttcgtc gcgccgcaaa ccgccacccg cagtttccgc agtacaacta ccagacgctg 3301 gtgccggaga atgaggcagc aggcaccgcg gtgctacgcg tggttgctca ggacccggac 3361 gccggcgagg ccgggcgcct agtctactcg ctggcggcac tcatgaacag ccgctcgctg 3421 gagctgttca gcatcgaccc gcagagcggc cttatccgta cggcggcagc tctggaccgc 3481 gagagcatgg agcgtcacta cctgcgtgtg accgcgcagg accacgggtc gccgcgcctc 3541 tcggccacca cgatggtggc cgtgacagta gccgaccgca acgaccactc gccggttttt 3601 gagcaagcgc agtaccggga gacccttcgc gagaatgtgg aggagggcta ccctatcctg 3661 cagctgcgtg ccactgacgg cgacgcgccc cccaacgcca acctgcgcta ccgcttcgtg 3721 gggccgccag ctgcgcgcgc tgcagctgcc gccgccttcg agattgatcc acgctccggc 3781 ctcatcagca ccagcggccg agtggaccgc gagcacatgg aaagctatga gctggtggtg 3841 gaagccagcg accagggcca ggaacccggg ccgcgctcgg ccactgtgcg cgtacacata 3901 actgtgctag acgagaacga caatgctcct cagttcagcg agaagcgcta cgtggcgcag 3961 gtgcgcgagg atgtgcgccc ccacacagtc gtgctgcgcg tcacggccac tgaccgggac 4021 aaggacgcca acggattggt gcactacaac atcatcagtg gcaatagccg tggacacttt 4081 gccatcgaca gcctcactgg cgagatccag gtggtggcac ctctggactt cgaggcagag 4141 agagagtatg ccttgcgcat cagggcgcag gatgctggcc ggccaccgct gtccaacaac 4201 acgggcctgg ccagcatcca ggtggtggac atcaatgacc acattcctat ttttgtcagc 4261 acgcccttcc aagtttctgt cttggaaaat gctcccttgg gtcactcagt catccacatt 4321 caggcagtcg atgcagacca tggggagaat gccagattgg agtactccct aactggtgtg 4381 gcacctgata ctccttttgt gataaacagc gccactggct gggtctctgt gagtggtccc 4441 ctggaccgtg agtctgtgga gcattacttc tttggtgtgg aggctcgaga ccatggctca 4501 cccccactct ctgcctcagc cagtgtcacc gtgactgtgc tggacgttaa tgacaatcgg 4561 cctgagttca caatgaagga gtaccaccta cgactgaatg aggatgcagc tgtgggcacc 4621 agtgtggtca gcgtgaccgc agtagaccgt gatgccaaca gtgccatcag ctaccagatc 4681 acaggcggca acacccggaa tcgctttgcc atcagcaccc aggggggtgt gggtctggtg 4741 actctggctc tgccactgga ctacaagcag gaacgctact tcaagctggt actaactgca 4801 tctgaccgtg cccttcatga tcactgctat gtgcacatca acatcacaga tgccaacact 4861 catcggccgg tctttcaaag tgcccactac tcagtgagtg tgaatgaaga tcggccaatg 4921 ggtagcacca tagtggtcat cagtgcctct gatgatgacg tgggtgagaa tgctcgtatc 4981 acctatctcc tggaggacaa cctgccccag ttccgcattg atgcagactc aggagccatt 5041 acattacagg ccccattaga ctatgaggac caggtgacct acaccctggc tatcacagct 5101 cgggacaatg gcatcccaca gaaggcagac actacttatg tggaggtgat ggtcaatgac 5161 gtgaatgaca atgctccaca atttgtggcc tcccactata cagggctggt ctctgaggat 5221 gccccacctt tcaccagtgt cctgcagatc tcagccactg accgggatgc tcatgccaat 5281 ggccgggtcc agtacacttt ccagaatggt gaagatgggg atggagattt taccattgag 5341 cccacctctg gaattgtccg tacagtaagg cggctagacc gggaggcagt atcagtgtat 5401 gagttgactg cctacgcagt ggacagaggt gtgcccccac tccggactcc agtcagtatc 5461 caggtgatgg tgcaggatgt gaacgacaat gcacctgtct tcccagctga ggagtttgag 5521 gtgcgggtga aagagaatag cattgtgggc tcagtggtgg cccagatcac tgcagtggac 5581 cctgacgaag gccccaatgc ccatataatg taccagatcg tggaggggaa catccctgag 5641 ctgttccaaa tggacatctt ctctggagaa ctgacggcac tcattgacct agactatgag 5701 gctcgccaag aatatgtgat tgtggtgcag gccacatctg ctcctttggt cagccgggcc 5761 actgtgcacg tccgcctggt tgaccagaat gacaacagcc ctgtgctcaa caacttccag 5821 atcctcttca acaactatgt atccaaccgt tcagacacct tcccgtcggg cattattggg 5881 cgcatcccag cttatgaccc cgatgtctcc gaccacctct tctactcctt tgagcgtggc 5941 aatgagctgc agctgctggt agtcaaccag accagtgggg agctgcgact cagccgaaag 6001 ctagacaata accgcccact ggtggcctcc atgttggtga ctgtcacaga tggcctgcac 6061 agcgtgacgg cgcagtgtgt gctgcgcgtg gtcatcatca cggaggagtt gctggccaac 6121 agcctgaccg tgcgccttga gaacatgtgg caggagcgct tcctgtcacc gctgctgggc 6181 cgcttcctcg agggcgtggc tgcggtgctc gctacgcccg ctgaggacgt cttcatcttc 6241 aacatccaga acgacacaga cgtagggggc accgtgctca atgtgagttt ctcggcgcta 6301 gctccacgtg gggccggggc gggcgctgca gggccctggt tcagctccga ggagctgcag 6361 gagcagttgt acgtgcgccg ggcggcgctg gcggctcgct ccctgctcga cgtactgccc 6421 ttcgacgaca acgtgtgcct gcgagagccc tgtgagaact acatgaaatg cgtgtccgtg 6481 ctccgctttg actcgtccgc gcccttcctg gcctcggcct ccacgctgtt ccgacccatc 6541 cagcccatcg ctggcctgcg ctgccgctgc ccgcccggat tcacgggaga cttttgcgag 6601 accgagctcg acctctgcta ctccaaccca tgtcgcaacg gcggagcctg cgcgcggcgc 6661 gagggaggct acacgtgcgt ctgccgcccg cgcttcaccg gagaggactg cgagctggac 6721 accgaggccg gccgctgcgt gccgggcgtc tgccgcaacg ggggcacctg caccgacgcg 6781 cccaacggcg gctttcgctg ccagtgcccg gcaggcggcg ccttcgaggg cccgcgctgc 6841 gaggtggctg cgcgctcctt cccgcccagt tcgttcgtca tgtttcgcgg cctgcggcag 6901 cgattccacc ttacgctgtc cctctcgttc gcgacagtgc agcagagcgg gctgctcttc 6961 tacaacgggc gcctgaacga gaagcacgac ttcctggccc tggaactcgt ggctggccaa 7021 gtgcggctca catattccac gggtgaatcc aacaccgtgg tcagccccac agttccaggg 7081 ggcttgagtg acgggcaatg gcatacagtg catctgagat actacaacaa gccccggaca 7141 gatgccctag ggggtgcaca gggcccctcc aaggacaagg tggctgtgct aagcgtggat 7201 gattgtgatg tggccgtggc tctgcagttt ggtgctgaga ttggcaacta ctcatgcgcg 7261 gctgctggtg tgcaaacaag ctccaagaag tccctggacc tgacgggccc tcttcttctg 7321 ggaggtgtcc ccaacctccc cgagaacttc cccgtatccc ataaggactt catcggctgt 7381 atgcgggacc tgcacattga tggccgccga gtggacatgg cggcttttgt cgcaaataat 7441 ggcaccatgg caggctgcca agccaagcta cacttttgtg actcaggccc ctgcaagaac 7501 agtggcttct gctcggagcg ctggggcagc ttcagctgcg actgccctgt gggcttcggc 7561 ggcaaagact gtcagcttac tatggcccat ccccaccatt tccgtggcaa cggcacactg 7621 agctggaact ttggaagtga catggctgtg tctgtgccat ggtacctggg gctggcattt 7681 cggacacggg caacgcaggg ggtcctgatg caagtgcagg ctgggccaca cagcacgctc 7741 ctttgccagc tagatcgggg gttactgtct gtgacagtga ccaggggctc gggccgtgct 7801 tcccatctcc ttctggacca ggtgactgtc agtgatggcc ggtggcacga tctgcggctg 7861 gagttgcagg aggaaccagg tggccggcgg ggccaccatg tccttatggt ctcactggac 7921 tttagcctct tccaggacac catggcggtg gggagtgagc tgcagggcct gaaggtaaag 7981 cagctccacg tgggaggcct gccccccggc agtgcagagg aggctcctca gggtctggtt 8041 ggctgcatcc agggggtgtg gctcggctcc acaccctctg gctccccggc cctgctaccc 8101 cccagccacc gagtgaatgc ggagcctggc tgtgttgtga ccaacgcctg tgcctctggg 8161 ccctgcccac ctcacgcaga ctgccgggac ctctggcaga ccttttcttg cacctgccag 8221 ccaggttact acggcccagg ctgtgtggat gcctgcctcc tgaacccctg tcagaaccag 8281 ggatcatgcc ggcacctgcc aggagccccc catggctata cctgtgactg tgtgggtggc 8341 tatttcgggc accactgtga gcacaggatg gaccagcagt gcccacgggg ctggtggggg 8401 agcccaacct gtggcccctg caactgtgat gttcacaaag gttttgatcc caactgcaac 8461 aagacaaatg ggcagtgtca ctgcaaggag ttccactacc gaccgcgggg cagtgactct 8521 tgcctcccat gtgactgcta ccctgtgggc tccacctcgc gctcatgtgc accccacagc 8581 gggcagtgcc cctgtcgccc aggagccctt ggccgccagt gcaacagctg tgacagtccc 8641 ttcgcagagg tgacagccag cggctgccgg gtgctctatg atgcctgccc taagtccctg 8701 agatctggtg tgtggtggcc ccagacaaag tttggcgtcc tggccacagt gccctgtccc 8761 cggggggccc tgggtgctgc tgtgcggctg tgtgatgagg cccagggttg gctggagccc 8821 gacctcttca actgtacctc ccctgccttt cgagagctca gtctgctgct ggatggccta 8881 gagctgaaca agacggcact ggataccatg gaggccaaga agctggctca gcggctacgg 8941 gaggtgactg gccacactga ccactatttt agccaagatg ttcgagtcac tgcccgcctg 9001 ctggcccacc tgctggcctt cgagagccat cagcagggct tcgggctgac agccacacag 9061 gatgcccact tcaatgagaa tctgctgtgg gccggctctg cactgcttgc cccagagaca 9121 ggggacttgt gggcggcgct ggggcagcgg gcccctgggg gctccccagg cagcgcggga 9181 ctggtgaggc acctggagga gtatgcagcc acactcgcaa ggaatatgga actcacatac 9241 ctgaatccca tggggctggt gacgcctaat atcatgctca gcattgaccg catggagcac 9301 cccagttctc cccggggggc ccgtcgctac cctcgctacc atagcaacct ctttcgaggc 9361 caggatgcct gggatcctca cacccatgtg ctgctgcctt cccagtcccc acggccatcc 9421 ccatctgaag ttctgcccac aagcagcagc atagaaaact ccaccacctc aagtgtggtc 9481 cccccaccag ccccgccaga gccagagcct gggatctcca ttatcattct cctcgtttac 9541 cgcaccttag ggggactgct ccctgcccag ttccaggcag aacgccgagg tgccaggctt 9601 cctcagaacc ccgtcatgaa ctccccggtg gtcagcgtgg ctgtgttcca cggacgcaac 9661 ttcctaaggg gaatcctgga gtcccccatc agcctagagt ttcgcctgct acagacagcg 9721 aatcggagca aggcgatctg tgtgcagtgg gacccacctg gcctggcgga gcagcatggt 9781 gtgtggacag cacgggactg cgagctggtg cacaggaatg ggtcccacgc acggtgtcgc 9841 tgcagccgga cagggacctt tggggtcctc atggatgcct ctccccgtga gaggctggag 9901 ggcgacctgg agctgctggc tgtgttcacc cacgtggtcg tggctgtgtc tgtggctgcg 9961 ctggtgctga ctgcagccat cctgctgagc ctgcgcagcc tcaagtccaa tgtgcgtggg 10021 atccatgcca atgtggcagc cgccctgggg gtggcagagc tcctcttcct gctggggatt 10081 cacaggaccc acaatcagct ggtgtgcact gcagtcgcca tcctcctgca ctacttcttc 10141 ctcagcacct tcgcgtggct cttcgtgcag gggctgcacc tctaccgcat gcaggttgag 10201 ccacgcaacg tggaccgcgg cgccatgcgc ttctaccatg ccctgggctg gggcgtccct 10261 gctgtgctgc tgggccttgc tgtgggcctg gaccctgagg gctatgggaa ccctgacttc 10321 tgctggatct cagtccacga gcccctcatc tggagctttg ctggccctgt tgtcctggtc 10381 atagtgatga acgggaccat gtttctcctc gctgcccgca catcctgctc cacagggcag 10441 agggaggcca agaagacctc tgcactgacc cttcgcagct ccttcctgct gcttctgctg 10501 gtcagtgcct cctggctctt tgggctcctg gcagtcaacc acagcatcct agccttccac 10561 tacctccatg ctggactctg cggcctccag ggcctggcgg tgctgctgct cttctgtgtc 10621 ctaaatgcag atgctcgggc tgcctggatg ccagcctgtc tgggcaggaa ggcagcgcct 10681 gaggaggcaa ggccagcacc tgggctggga cctggggcct acaacaacac ggctctcttt 10741 gaggagagtg gcctcatccg catcactctg ggcgcctcca ccgtctcctc tgtgagcagt 10801 gcccgctccg gccggaccca ggaccaggac agccagcggg gccgcagcta cctcagggac 10861 aatgtcctgg ttcgacatgg ctcagccgct gaccacactg accacagcct ccaggctcat 10921 gctggcccca ctgacctgga cgtggccatg ttccatcgag atgctggcgc agactccgac 10981 tctgacagtg acctgtcctt ggaggaggag aggagtctct ccattccatc ttcagaaagc 11041 gaggacaatg gccggacgcg ggggcgcttc caacggccac tctgccgagc agcccagagt 11101 gagaggctcc tcacccaccc caaagatgtg gatggcaatg acctcctgtc ctactggcca 11161 gccctggggg agtgcgaggc agccccctgt gctctgcaga cttggggctc tgaaaggcgc 11221 ctggggctgg acaccagcaa ggatgcagct aacaacaacc agccagaccc ggccctgacc 11281 agtggggatg agacttctct gggccgggcc cagcgccaga ggaaaggcat cctgaagaac 11341 cggttgcaat acccactggt gccacagacc cgaggtgccc ctgagctgtc ctggtgccgt 11401 gcagccacct tgggccaccg tgctgtgcca gctgcctctt acggtcgcat ctatgctggc 11461 gggggcacgg gcagcctttc acagccagcc agccgctact cttctagaga acagctggac 11521 ctgctcctcc ggcggcaact gagccgtgag cgactagagg aagcccctgc ccctgttcta 11581 cgtcccctga gccggccagg gtcccaggaa tgcatggatg ctgcaccagg ccgactggag 11641 cccaaagatc ggggcagcac cctgccacgg aggcagccac ctcgggacta ccctggcgcc 11701 atggctggcc gcttcgggtc acgggatgcg ctcgacttag gggcacctcg agagtggttg 11761 agcacgctgc ctccgccccg ccgcacccgg gaccttgacc cacagccccc acctctgccc 11821 ctgtctcccc agcggcaact ctcaagggac cccctcttgc catcccggcc gctggactct 11881 ctgtctagga gctcgaactc tcgggagcag ctggaccagg tgcctagccg gcacccctca 11941 cgagaagccc ttgggccact cccgcagctg ctcagagcta gggaggactc ggtcagtggc 12001 cccagccatg gcccctccac agaacagttg gacattcttt cctccatcct tgcctctttc 12061 aactcctcgg ccctctcctc tgtgcaatct tcaagcacac ccttgggccc tcacaccact 12121 gccacacctt ctgccacagc ctctgtgctt gggccctcca cgccacgttc tgccacgtct 12181 cacagcatct cggagctgtc gccagactca gaagttccca gaagtgaggg tcactccGGC 12241 TCGAGTGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT 12301 GACGATGACA AGGGATCCga gggcagagga agtcttctaa catgcggtga cgtggaggag 12361 aatcccggcc ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 12421 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 12481 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 12541 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 12601 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 12661 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 12721 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 12781 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 12841 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 12901 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 12961 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 13021 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 13081 ctgtacaagt agGCGGCCGC GAAGGATCTG CGATCGCTCC GGTGCCCGTC AGTGGGCAGA 13141 GCGCACATCG CCCACAGTCC CCGAGAAGTT GGGGGGAGGG GTCGGCAATT GAACGGGTGC 13201 CTAGAGAAGG TGGCGCGGGG TAAACTGGGA AAGTGATGTC GTGTACTGGC TCCGCCTTTT 13261 TCCCGAGGGT GGGGGAGAAC CGTATATAAG TGCAGTAGTC GCCGTGAACG TTCTTTTTCG 13321 CAACGGGTTT GCCGCCAGAA CACAGCTGAA GCTTCGAGGG GCTCGCATCT CTCCTTCACG 13381 CGCCCGCCGC CCTACCTGAG GCCGCCATCC ACGCCGGTTG AGTCGCGTTC TGCCGCCTCC 13441 CGCCTGTGGT GCCTCCTGAA CTGCGTCCGC CGTCTAGGTA AGTTTAAAGC TCAGGTCGAG 13501 ACCGGGCCTT TGTCCGGCGC TCCCTTGGAG CCTACCTAGA CTCAGCCGGC TCTCCACGCT 13561 TTGCCTGACC CTGCTTGCTC AACTCTACGT CTTTGTTTCG TTTTCTGTTC TGCGCCGTTA 13621 CAGATCCAAG CTGTGACCGG CGCCTACGCT AGATGACCGA GTACAAGCCC ACGGTGCGCC 13681 TCGCCACCCG CGACGACGTC CCCCGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT 13741 ACCCCGCCAC GCGCCACACC GTCGACCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC 13801 AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG 13861 GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG 13921 AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG 13981 AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG 14041 TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG 14101 CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT 14161 TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA 14221 CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GAGTCGACAA TCAACCTCTG GATTACAAAA 14281 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG 14341 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT 14401 TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG 14461 GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT 14521 GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG 14581 CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG 14641 TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC 14701 TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC 14761 GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC 14821 GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GTACCTTTAA GACCAATGAC TTACAAGGCA 14881 GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC 14941 CAACGAAAAT AAGATCTGCT TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA 15001 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT 15061 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC 15121 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTAGTA GTTCATGTCA TCTTATTATT 15181 CAGTATTTAT AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGAACTT GTTTATTGCA 15241 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT 15301 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGCTC 15361 TAGCTATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA 15421 TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT 15481 GAGGAGGCTT TTTTGGAGGC CTAGACTTTT GCAGAGACGG CCCAAATTCG TAATCATGGT 15541 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 15601 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 15661 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 15721 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 15781 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 15841 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 15901 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 15961 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 16021 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 16081 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 16141 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 16201 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 16261 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 16321 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 16381 GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 16441 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC 16501 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 16561 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 16621 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 16681 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 16741 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 16801 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 16861 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 16921 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 16981 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 17041 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 17101 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 17161 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 17221 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 17281 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 17341 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 17401 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 17461 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 17521 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 17581 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 17641 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG 17701 AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC 17761 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 17821 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 17881 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 17941 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 18001 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 18061 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 18121 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.