pLV3-CMV-Chmp1b-3×FLAG-EGFP-EF1a-Puro plasmid

PVT109278

pLV3-CMV-Chmp1b-3×FLAG-EGFP-EF1a-Puro plasmid Information

Plasmid name: pLV3-CMV-Chmp1b(mouse)-3×FLAG-EGFP-EF1a-Puro
Alias: NM_024190.2;2810405I11Rik; Chmp1b1
Gene lenght: 597
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Chmp1b-3×FLAG-EGFP-EF1a-Puro plasmid Description

pLV3-CMV-Chmp1b-3×FLAG-EGFP-EF1a-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Chmp1b-3×FLAG-EGFP-EF1a-Puro plasmid Sequence

1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 181 tgggaggttt atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat 241 ccacgctgtt ttgacctcca tagaagattc tagagctagc gaattcGCCA CCatgtccaa 301 catggagaag catctgttca acctgaaatt cgcggccaaa gaactgaacc ggagttccaa 361 aaaatgcgac aaggaagaaa aggccgaaaa ggccaaaatt aaaaaggcca ttcagaaggg 421 caacatggaa gttgcgagga ttcacgccga aaatgccatc cgccagaaga atcaaggggt 481 gaacttcttg agaatgagtg cacgagtgga tgcggtggcg gcccgtgtcc agactgcagt 541 gacgatgggc aaagtgacca agtccatggc gggtgtggtt aagtcgatgg acgcgacgtt 601 gaaaagtatg aatctggaga agatctccgc tttgatggac aaattcgaac accagttcga 661 gactctggac gtccagacgc agcaaatgga agacacaatg agcagcacga cgacgctgac 721 cactccccag aaccaggtgg atatgctgct ccaggaaatg gcagatgagg cgggcctcga 781 tctcaacatg gagctgcctc agggccagac cggttccgtg ggaacgagcg tggcttcggc 841 tgagcaagat gaactgtccc agagactggc ccgccttcgg gatcaagtcC TCGAGGACTA 901 CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA 961 Ggggcgcgcc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 1021 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 1081 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 1141 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga 1201 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 1261 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 1321 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 1381 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 1441 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt 1501 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 1561 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 1621 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 1681 gtacaagtaa gcggccgcga aggatctgcg atcgctccgg tgcccgtcag tgggcagagc 1741 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct 1801 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 1861 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca 1921 acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg 1981 cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg 2041 cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac 2101 cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt 2161 gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca 2221 gatccaagct gtgaccggcg cctacgctag atgaccgagt acaagcccac ggtgcgcctc 2281 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac 2341 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa 2401 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 2461 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 2521 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 2581 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 2641 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 2701 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 2761 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 2821 tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc aacctctgga ttacaaaatt 2881 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 2941 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 3001 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 3061 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 3121 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 3181 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 3241 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg 3301 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 3361 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 3421 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc 3481 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 3541 acgaagataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 3601 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 3661 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 3721 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 3781 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 3841 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 3901 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 3961 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 4021 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 4081 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa 4141 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 4201 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 4261 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 4321 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 4381 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 4441 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 4501 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 4561 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 4621 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 4681 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 4741 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 4801 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 4861 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 4921 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 4981 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 5041 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 5101 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 5161 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 5221 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 5281 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 5341 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 5401 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 5461 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 5521 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 5581 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 5641 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 5701 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 5761 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 5821 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 5881 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 5941 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 6001 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 6061 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 6121 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 6181 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 6241 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 6301 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 6361 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 6421 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 6481 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 6541 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 6601 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 6661 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 6721 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctgac 6781 gcgtgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac 6841 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg 6901 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat 6961 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct 7021 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 7081 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 7141 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc 7201 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct 7261 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 7321 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 7381 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 7441 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 7501 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 7561 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 7621 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 7681 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 7741 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 7801 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 7861 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc 7921 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 7981 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 8041 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 8101 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 8161 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 8221 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 8281 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 8341 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 8401 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 8461 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag 8521 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt 8581 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca 8641 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt 8701 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 8761 atctacgtat tagtcatcgc tattaccatg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.