pLV3-CMV-CRABP2-3×FLAG-EF1a-CopGFP-T2A-Puro plasmid

PVT110693

pLV3-CMV-CRABP2-3×FLAG-EF1a-CopGFP-T2A-Puro plasmid Information

Plasmid name: pLV3-CMV-CRABP2(human)-3×FLAG-EF1a-CopGFP-T2A-Puro
Alias: NM_001878.4;CRABP-II; RBP6
Gene lenght: 414
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CRABP2-3×FLAG-EF1a-CopGFP-T2A-Puro plasmid Description

pLV3-CMV-CRABP2-3×FLAG-EF1a-CopGFP-T2A-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-CRABP2-3×FLAG-EF1a-CopGFP-T2A-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtttatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc gccaccatgc ccaacttctc tggcaactgg aaaatcatcc gatcggaaaa 2341 cttcgaggaa ttgctcaaag tgctgggggt gaatgtgatg ctgaggaaga ttgctgtggc 2401 tgcagcgtcc aagccagcag tggagatcaa acaggaggga gacactttct acatcaaaac 2461 ctccaccacc gtgcgcacca cagagattaa cttcaaggtt ggggaggagt ttgaggagca 2521 gactgtggat gggaggccct gtaagagcct ggtgaaatgg gagagtgaga ataaaatggt 2581 ctgtgagcag aagctcctga agggagaggg ccccaagacc tcgtggacca gagaactgac 2641 caacgatggg gaactgatcc tgaccatgac ggcggatgac gttgtgtgca ccagggtcta 2701 cgtccgagag ggatccGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA 2761 CTACAAGGAT GACGATGACA AGTGAgcggc cgcGaaggat ctgcgatcgc tccggtgccc 2821 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca 2881 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact 2941 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga 3001 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca 3061 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg 3121 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa 3181 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc 3241 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg 3301 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga 3361 gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg 3421 gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca 3481 acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga 3541 tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc 3601 acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg 3661 tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg 3721 tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca 3781 acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc 3841 gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact 3901 tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc 3961 gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca 4021 agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca 4081 ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg 4141 acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc 4201 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca 4261 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct 4321 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg 4381 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc 4441 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc 4501 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg 4561 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc 4621 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc 4681 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca 4741 tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga 4801 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 4861 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 4921 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 4981 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 5041 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 5101 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 5161 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 5221 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 5281 ctctgcggcc tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc 5341 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 5401 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc 5461 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 5521 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 5581 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 5641 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 5701 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 5761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 5821 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 5881 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 5941 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 6001 aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt 6061 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 6121 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 6181 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 6241 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 6301 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 6361 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 6421 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 6481 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 6541 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 6601 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 6661 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 6721 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 6781 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 6841 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 6901 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 6961 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7021 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 7081 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 7141 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 7201 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 7261 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 7321 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 7381 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 7441 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 7501 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 7561 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 7621 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 7681 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 7741 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 7801 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 7861 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 7921 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 7981 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8041 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 8101 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 8161 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 8221 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 8281 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 8341 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 8401 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 8461 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 8521 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 8581 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 8641 ttgtaaaacg acggccagtg ccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.