pLV3-CMV-Cyp17a1-EF1a-CopGFP-Puro plasmid

PVT111013

pLV3-CMV-Cyp17a1-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Cyp17a1(mouse)-EF1a-CopGFP-Puro
Alias: NM_007809.3;Cyp17; p450c17
Gene lenght: 1521
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Cyp17a1-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-Cyp17a1-EF1a-CopGFP-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-Cyp17a1-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgt gggaacttgt gggtctcttg 2341 ctgctcatcc tggcctattt cttttggccc aagtcaaaga cacctaatgc caagttcccc 2401 aggagccttc cattcctgcc cctggtgggt agtctaccgt ttctccccag acgtggtcat 2461 atgcatgcca acttcttcaa gctgcaggaa aagtatggtc ccatctattc tcttcgcctg 2521 ggtaccacaa ctgcagtgat tgtcggtcac tatcagctgg ccagagaagt gctcgtgaag 2581 aaggggaaag aattctctgg tcggccccag atggtgactc taggcctctt gtcggaccaa 2641 ggaaaaggcg tcgcctttgc ggatagtagt agctcctggc agctgcaccg gaagctggta 2701 ttcagcacct tttccctgtt cagggatgac cagaaactgg agaagatgat atgtcaggaa 2761 gccaactcac tgtgtgactt gatacttaca tacgacgggg agtcccgaga tctgtctacg 2821 ctcatcttca agtcagtaat caatatcatc tgtaccatct gcttcaacat ctcttttgag 2881 aacaaggatc cgatactgac taccatacag acctttacag agggtattgt ggatgtcctg 2941 ggccacagcg atctggtgga catattcccg tggttgaaga tttttcccaa taaaaacttg 3001 gaaatgataa aggaacacac taaaattcga gaaaaaacac tggttgaaat gtttgaaaaa 3061 tgcaaggaga aattcaatag tgaatctctc tccagcctga cagacattct gatacaagcc 3121 aagatgaatg cagaaaataa taacactggg gaaggccagg acccaagtgt gttctcagat 3181 aagcatatcc ttgtcacggt gggagacatc tttggggcag gcatagagac aactagctct 3241 gtgctgaact ggatcctggc tttcctggtg cacaatcctg aggtgaagag gaagatccaa 3301 aaggagattg accagtatgt aggcttcagt cgaacaccgt ctttcaatga ccggactcac 3361 ctcctcatgc tggaggccac tatccgagaa gtgcttcgta tcaggccggt ggcccccttg 3421 ctcatcccac acaaggctaa cattgactcc agcattggag agtttgccat cccgaaggac 3481 acacatgtga tcatcaatct ctgggcactg catcacgata aaaatgaatg ggaccagcca 3541 gatcggttta tgcctgagcg cttcttagat ccaacaggaa gccatctcat tacacccaca 3601 cccagttatt tgcccttcgg agctggtccc cgatcgtgca ttggagaggc tctggcccgg 3661 caggagctct ttatcttcat ggccttgctg ctgcagaggt ttgactttga tgtgtcagat 3721 gacaaacagc tgccctgtct ggtgggtgac cccaaggtgg tctttctgat cgaccctttc 3781 aaagtgaaaa tcacagtgcg acaagcatgg aaggatgcac aggttgaggt tagcacctag 3841 gatccCTGCG ATATCCAGCg cggccgcGaa ggatctgcga tcgctccggt gcccgtcagt 3901 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa 3961 cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc 4021 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc 4081 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc 4141 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc 4201 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca 4261 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct 4321 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc 4381 gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg 4441 agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg aacggcgtgg 4501 agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg accaacaaga 4561 tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac gtgatgggct 4621 acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc ctgcacgcca 4681 tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc ggcgtgctgc 4741 acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg 4801 gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc agcaacgcca 4861 ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc gcccgcacct 4921 tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg cacttcaaga 4981 gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg 5041 aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc ttcaagaccc 5101 ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg 5161 gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg 5221 aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 5281 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 5341 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 5401 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg 5461 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 5521 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 5581 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc 5641 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 5701 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 5761 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 5821 gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 5881 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 5941 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 6001 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 6061 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 6121 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 6181 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt 6241 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 6301 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 6361 ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt tggccgcctc 6421 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 6481 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt 6541 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 6601 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 6661 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 6721 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 6781 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa 6841 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 6901 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc 6961 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 7021 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 7081 agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa 7141 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 7201 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 7261 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 7321 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 7381 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 7441 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 7501 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 7561 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 7621 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 7681 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 7741 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 7801 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 7861 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 7921 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 7981 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 8041 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 8101 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 8161 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 8221 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 8281 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 8341 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 8401 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 8461 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 8521 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 8581 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 8641 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 8701 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 8761 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 8821 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 8881 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 8941 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 9001 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 9061 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 9121 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 9181 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 9241 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 9301 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 9361 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 9421 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 9481 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 9541 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa 9601 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 9661 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 9721 aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.