pLV3-CMV-CYP1A2-mCherry-Puro plasmid

PVT90057

pLV3-CMV-CYP1A2-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-CYP1A2(human)-mCherry-Puro
Alias: NM_000761.5;CP12; CYPIA2; P3-450; P450(PA)
Gene lenght: 1551bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CYP1A2-mCherry-Puro plasmid Description

pLV3-CMV-CYP1A2-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-CYP1A2-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggcatt gtcccagtct gttcccttct cggccacaga 2341 gcttctcctg gcctctgcca tcttctgcct ggtattctgg gtgctcaagg gtttgaggcc 2401 tcgggtcccc aaaggcctga aaagtccacc agagccatgg ggctggccct tgctcgggca 2461 tgtgctgacc ctggggaaga acccgcacct ggcactgtca aggatgagcc agcgctacgg 2521 ggacgtcctg cagatccgca ttggctccac gcccgtgctg gtgctgagcc gcctggacac 2581 catccggcag gccctggtgc ggcagggcga cgatttcaag ggccggcctg acctctacac 2641 ctccaccctc atcactgatg gccagagctt gaccttcagc acagactctg gaccggtgtg 2701 ggctgcccgc cggcgcctgg cccagaatgc cctcaacacc ttctccatcg cctctgaccc 2761 agcttcctca tcctcctgct acctggagga gcatgtgagc aaggaggcta aggccctgat 2821 cagcaggttg caggagctga tggcagggcc tgggcacttc gacccttaca atcaggtggt 2881 ggtgtcagtg gccaacgtca ttggtgccat gtgcttcgga cagcacttcc ctgagagtag 2941 cgatgagatg ctcagcctcg tgaagaacac tcatgagttc gtggagactg cctcctccgg 3001 gaaccccctg gacttcttcc ccatccttcg ctacctgcct aaccctgccc tgcagaggtt 3061 caaggccttc aaccagaggt tcctgtggtt cctgcagaaa acagtccagg agcactatca 3121 ggactttgac aagaacagtg tccgggacat cacgggtgcc ctgttcaagc acagcaagaa 3181 ggggcctaga gccagcggca acctcatccc acaggagaag attgtcaacc ttgtcaatga 3241 catctttgga gcaggatttg acacagtcac cacagccatc tcctggagcc tcatgtacct 3301 tgtgaccaag cctgagatac agaggaagat ccagaaggag ctggacactg tgattggcag 3361 ggagcggcgg ccccggctct ctgacagacc ccagctgccc tacttggagg ccttcatcct 3421 ggagaccttc cgacactcct ccttcttgcc cttcaccatc ccccacagca caacaaggga 3481 cacaacgctg aatggcttct acatccccaa gaaatgctgt gtcttcgtaa accagtggca 3541 ggtcaaccat gacccagagc tgtgggagga cccctctgag ttccggcctg agcggttcct 3601 caccgccgat ggcactgcca ttaacaagcc cttgagtgag aagatgatgc tgtttggcat 3661 gggcaagcgc cggtgtatcg gggaagtcct ggccaagtgg gagatcttcc tcttcctggc 3721 catcctgcta cagcaactgg agttcagcgt gccgccgggc gtgaaagtcg acctgacccc 3781 catctacggg ctgaccatga agcacgcccg ctgtgaacat gtccaggcgc ggctgcgctt 3841 ctccatcaat tgaggatccg cggccgcGaa ggatctgcga tcgctccggt gcccgtcagt 3901 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa 3961 cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc 4021 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc 4081 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc 4141 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc 4201 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca 4261 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct 4321 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc 4381 gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg gtgagcaagg 4441 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg 4501 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg 4561 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca 4621 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc 4681 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg 4741 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct 4801 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga 4861 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg 4921 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca 4981 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg 5041 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc 5101 gccactccac cggcggcatg gacgagctgt acaagggatc tcgcgagggc agaggaagtc 5161 ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac aagcccacgg 5221 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg 5281 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg 5341 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 5401 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 5461 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 5521 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 5581 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 5641 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc 5701 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac 5761 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa 5821 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 5881 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 5941 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 6001 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 6061 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 6121 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 6181 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc 6241 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 6301 gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct cagacgagtc 6361 ggatctccct ttggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag 6421 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc 6481 aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag 6541 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 6601 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 6661 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc 6721 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag 6781 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 6841 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct 6901 agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 6961 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 7021 aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc 7081 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 7141 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 7201 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 7261 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 7321 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 7381 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 7441 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 7501 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 7561 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 7621 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 7681 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 7741 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 7801 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 7861 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 7921 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 7981 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 8041 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 8101 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 8161 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 8221 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 8281 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 8341 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 8401 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 8461 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 8521 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 8581 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 8641 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 8701 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 8761 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 8821 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 8881 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 8941 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 9001 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 9061 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 9121 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 9181 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga 9241 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct 9301 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 9361 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 9421 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 9481 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca 9541 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt 9601 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt 9661 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.