pLV3-CMV-EDN1-3×FLAG-8×His-CopGFP-Puro plasmid

PVT111708

pLV3-CMV-EDN1-3×FLAG-8×His-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-EDN1(human)-3×FLAG-8×His-CopGFP-Puro
Alias: NM_001955.5;ARCND3; ET1; HDLCQ7; PPET1; QME
Gene lenght: 636
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-EDN1-3×FLAG-8×His-CopGFP-Puro plasmid Description

pLV3-CMV-EDN1-3×FLAG-8×His-CopGFP-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-EDN1-3×FLAG-8×His-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg attatttgct catgattttc 2341 tctctgctgt ttgtggcttg ccaaggagct ccagaaacag cagtcttagg cgctgagctc 2401 agcgcggtgg gtgagaacgg cggggagaaa cccactccca gtccaccctg gcggctccgc 2461 cggtccaagc gctgctcctg ctcgtccctg atggataaag agtgtgtcta cttctgccac 2521 ctggacatca tttgggtcaa cactcccgag cacgttgttc cgtatggact tggaagccct 2581 aggtccaaga gagccttgga gaatttactt cccacaaagg caacagaccg tgaaaataga 2641 tgccaatgtg ctagccaaaa agacaagaag tgctggaatt tttgccaagc aggaaaagaa 2701 ctcagggctg aagacattat ggagaaagac tggaataatc ataagaaagg aaaagactgt 2761 tccaagcttg ggaaaaagtg tatttatcag cagttagtga gaggaagaaa aatcagaaga 2821 agttcagagg aacacctaag acaaaccagg tcggagacca tgagaaacag cgtcaaatca 2881 tcttttcatg atcccaagct gaaaggcaag ccctccagag agcgttatgt gacccacaac 2941 cgagcacatt ggGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 3001 AAGGATGACG ATGACAAGgg atccCACCAT CACCATCACC ATCACCATta gcggccgcGa 3061 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc 3121 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta 3181 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg 3241 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca 3301 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc 3361 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact 3421 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc 3481 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa 3541 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg 3601 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag 3661 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc 3721 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc 3781 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc 3841 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc 3901 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc 3961 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc 4021 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat 4081 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc 4141 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac 4201 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc 4261 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag 4321 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc 4381 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac 4441 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 4501 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 4561 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 4621 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 4681 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 4741 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 4801 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 4861 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 4921 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 4981 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg 5041 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 5101 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 5161 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 5221 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 5281 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 5341 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 5401 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 5461 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 5521 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct 5581 cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag accaatgact 5641 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 5701 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac 5761 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 5821 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 5881 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 5941 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg 6001 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 6061 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 6121 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 6181 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 6241 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt 6301 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 6361 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 6421 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 6481 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 6541 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 6601 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 6661 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 6721 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 6781 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 6841 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 6901 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 6961 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 7021 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 7081 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 7141 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 7201 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 7261 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 7321 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 7381 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 7441 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 7501 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 7561 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 7621 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 7681 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 7741 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 7801 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 7861 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 7921 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 7981 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 8041 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 8101 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 8161 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 8221 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 8281 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 8341 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 8401 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 8461 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 8521 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 8581 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 8641 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac 8701 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 8761 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 8821 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 8881 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.