pLV3-CMV-EN1-mCherry-Puro plasmid

PVT107105

pLV3-CMV-EN1-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-EN1(human)-mCherry-Puro
Alias: NM_001426.4;ENDOVESLB
Gene lenght: 1176
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F(HighGC???
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-EN1-mCherry-Puro plasmid Description

pLV3-CMV-EN1-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-EN1-mCherry-Puro plasmid Sequence

1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 361 gcgctaccgg ACTCAGATCT CGAGCTCAAG CTTCGAATTC TGCAGTCGAC gccaccatgg 421 aagaacagca gccggaacct aaaagtcagc gcgactcggc cctcggcgcg gcggcggcgg 481 cgactccggg cggcctcagc ctgagcctca gtccgggcgc cagcggcagc agcggcagcg 541 gcagcgatgg agacagcgtg ccggtgtccc cgcagcctgc gcccccctcg ccgcccgcgg 601 cgccttgcct gccgcccctg gcccaccacc cgcacctccc cccacacccc ccgcccccgc 661 cgcctcagca tctcgcggcg cctgctcacc agccgcagcc agcggcccag ctgcaccgca 721 ccaccaactt tttcatcgac aacatcctga ggccggactt cggctgcaaa aaggagcagc 781 cgccaccgca gcttctggtg gctgcggcgg ccagaggagg cgcaggagga ggaggccggg 841 tcgagcgtga cagaggccag actgccgcag gtagagaccc tgtccacccg ttgggcaccc 901 gggcgccagg cgctgcctcg ctcctgtgcg ccccggacgc gaactgtggc ccacccgacg 961 gctcccagcc agccgccgcc ggcgcgggcg cgtctaaagc tgggaacccg gctgcggcgg 1021 cggcggcggc cgcggcggca gtggcggcgg cggcggcggc cgcagcagcc aagccctcgg 1081 acaccggtgg cggcggcagt ggaggcggcg cggggagccc cggagcgcag ggcaccaaat 1141 acccggagca cggcaacccg gctatcctac ttatgggctc agccaacggc gggcccgtgg 1201 tcaaaactga ctcgcagcag cctctcgtat ggcccgcctg ggtgtactgc acacgttatt 1261 cggatcgtcc atcctccggt ccgcgcacca ggaagctgaa gaagaagaag aacgagaagg 1321 aggacaagcg gccgcggacc gcgttcacgg ccgagcagct gcagagactc aaggcggagt 1381 tccaggcaaa ccgctacatc acggagcagc ggcggcagac cctggcccag gaactcagcc 1441 tcaacgagtc ccagatcaag atctggttcc agaacaagcg cgccaagatc aagaaagcca 1501 caggcatcaa gaacggcctg gcgctgcacc tcatggccca gggactgtac aaccactcca 1561 ccaccacggt ccaggacaaa gacgagagcg agggatccGG AGGAGGGGGA AGCatACCGG 1621 TCGCCACCat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc 1681 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg 1741 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc 1801 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg 1861 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt 1921 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc 1981 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg 2041 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg 2101 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact 2161 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct 2221 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac 2281 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtGAA 2341 ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt 2401 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa 2461 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga 2521 gatccacttt ggccgcggct cgagggggtt ggggttgcgc cttttccaag gcagccctgg 2581 gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc 2641 tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct 2701 tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt 2761 cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg 2821 acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg 2881 ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg 2941 ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga 3001 gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggggga 3061 tccaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 3121 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 3181 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 3241 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt 3301 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat 3361 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 3421 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca 3481 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg 3541 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 3601 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg 3661 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg 3721 accccatgca tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 3781 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct 3841 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 3901 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 3961 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 4021 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 4081 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 4141 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 4201 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta 4261 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 4321 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 4381 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt 4441 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 4501 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 4561 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct 4621 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 4681 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 4741 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 4801 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 4861 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 4921 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 4981 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 5041 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac 5101 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 5161 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 5221 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 5281 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 5341 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 5401 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 5461 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 5521 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 5581 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 5641 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 5701 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 5761 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 5821 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 5881 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 5941 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 6001 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 6061 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 6121 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 6181 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 6241 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 6301 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 6361 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 6421 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 6481 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 6541 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 6601 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 6661 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 6721 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 6781 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 6841 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 6901 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 6961 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc 7021 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 7081 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 7141 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 7201 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga 7261 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac 7321 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga 7381 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca 7441 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct 7501 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 7561 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 7621 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 7681 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc 7741 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac 7801 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga 7861 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag 7921 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat 7981 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc 8041 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa 8101 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga 8161 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga 8221 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta 8281 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa 8341 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact 8401 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg 8461 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca 8521 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat 8581 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct 8641 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg 8701 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg 8761 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg 8821 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta 8881 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg 8941 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg 9001 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg 9061 aacggatctc gacggtatcg atcacgagac tagcctcgag cggccgcccc cttcaccgag 9121 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata 9181 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag 9241 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatca

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???