pLV3-CMV-FGL2-3×FLAG-CopGFP-Puro plasmid

PVT93169

pLV3-CMV-FGL2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-FGL2(human)-3×FLAG-CopGFP-Puro
Alias: NM_006682.3；FGL2；pT49；T49
Gene lenght: 1317bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FGL2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-FGL2-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-FGL2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCATGA AGCTGGCTAA CTGGTACTGG 2341 CTGAGCTCAG CTGTTCTTGC CACTTACGGT TTTTTGGTTG TGGCAAACAA TGAAACAGAG 2401 GAAATTAAAG ATGAAAGAGC AAAGGATGTC TGCCCAGTGA GACTAGAAAG CAGAGGGAAA 2461 TGCGAAGAGG CAGGGGAGTG CCCCTACCAG GTAAGCCTGC CCCCCTTGAC TATTCAGCTC 2521 CCGAAGCAAT TCAGCAGGAT CGAGGAGGTG TTCAAAGAAG TCCAAAACCT CAAGGAAATC 2581 GTAAATAGTC TAAAGAAATC TTGCCAAGAC TGCAAGCTGC AGGCTGATGA CAACGGAGAC 2641 CCAGGCAGAA ACGGACTGTT GTTACCCAGT ACAGGAGCCC CGGGAGAGGT TGGTGATAAC 2701 AGAGTTAGAG AATTAGAGAG TGAGGTTAAC AAGCTGTCCT CTGAGCTAAA GAATGCCAAA 2761 GAGGAGATCA ATGTACTTCA TGGTCGCCTG GAGAAGCTGA ATCTTGTAAA TATGAACAAC 2821 ATAGAAAATT ATGTTGACAG CAAAGTGGCA AATCTAACAT TTGTTGTCAA TAGTTTGGAT 2881 GGCAAATGTT CAAAGTGTCC CAGCCAAGAA CAAATACAGT CACGTCCAGT TCAACATCTA 2941 ATATATAAAG ATTGCTCTGA CTACTACGCA ATAGGCAAAA GAAGCAGTGA GACCTACAGA 3001 GTTACACCTG ATCCCAAAAA TAGTAGCTTT GAAGTTTACT GTGACATGGA GACCATGGGG 3061 GGAGGCTGGA CAGTGCTGCA GGCACGTCTC GATGGGAGCA CCAACTTCAC CAGAACATGG 3121 CAAGACTACA AAGCAGGCTT TGGAAACCTC AGAAGGGAAT TTTGGCTGGG GAACGATAAA 3181 ATTCATCTTC TGACCAAGAG TAAGGAAATG ATTCTGAGAA TAGATCTTGA AGACTTTAAT 3241 GGTGTCGAAC TATATGCCTT GTATGATCAG TTTTATGTGG CTAATGAGTT TCTCAAATAT 3301 CGTTTACACG TTGGTAACTA TAATGGCACA GCTGGAGATG CATTACGTTT CAACAAACAT 3361 TACAACCACG ATCTGAAGTT TTTCACCACT CCAGATAAAG ACAATGATCG ATATCCTTCT 3421 GGGAACTGTG GGCTGTACTA CAGTTCAGGC TGGTGGTTTG ATGCATGTCT TTCTGCAAAC 3481 TTAAATGGCA AATATTATCA CCAAAAATAC AGAGGTGTCC GTAATGGGAT TTTCTGGGGT 3541 ACCTGGCCTG GTGTAAGTGA GGCACACCCT GGTGGCTACA AGTCCTCCTT CAAAGAGGCT 3601 AAGATGATGA TCAGACCCAA GCACTTTAAG CCAggatccG ACTACAAAGA CCATGACGGT 3661 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGtagcg gccgcGaagg 3721 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 3781 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 3841 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 3901 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 3961 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 4021 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 4081 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 4141 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 4201 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 4261 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc 4321 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc 4381 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc 4441 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc 4501 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc 4561 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc 4621 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc 4681 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac 4741 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc 4801 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg 4861 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc 4921 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg 4981 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga 5041 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag 5101 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 5161 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 5221 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 5281 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 5341 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 5401 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 5461 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 5521 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 5581 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 5641 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat 5701 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 5761 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 5821 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 5881 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 5941 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 6001 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 6061 tccgtggtgt tgtcggggaa ATCATCGTCC TTTCCTtggc tgctcgcctg tgttgccacc 6121 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 6181 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctTcgcct tcgccctcag 6241 acgagtcgga tctccctttg gGccgcctcc ccgcctggta cctttaagac caatgactta 6301 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 6361 tcactcccaa cgaaGataag atctgctttt tgcttgtact gggtctctct ggttagacca 6421 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 6481 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 6541 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 6601 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 6661 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 6721 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 6781 ctggctctag ctaTCCCGCC CCTAACTCCG CCCAtcccgc ccctaactcc gcccagttcc 6841 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 6901 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 6961 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac 7021 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 7081 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 7141 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 7201 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 7261 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 7321 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 7381 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 7441 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 7501 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 7561 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 7621 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 7681 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 7741 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 7801 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 7861 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 7921 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 7981 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 8041 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 8101 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 8161 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 8221 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 8281 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 8341 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 8401 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 8461 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 8521 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 8581 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 8641 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 8701 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 8761 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 8821 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 8881 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 8941 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 9001 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 9061 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 9121 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 9181 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 9241 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 9301 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 9361 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 9421 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 9481 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 9541 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 9601 caagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???