pLV3-CMV-FLAG-Cebpb-EF1a-CopGFP-Puro plasmid

PVT97222

pLV3-CMV-FLAG-Cebpb-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-FLAG-Cebpb(mouse)-EF1a-CopGFP-Puro
Alias: NM_009883.4;C/EBPbeta; CRP2; IL-6DBP; LAP; LIP; NF-IL6;NF-M; Nfil6
Gene lenght: 891bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FLAG-Cebpb-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-FLAG-Cebpb-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-FLAG-Cebpb-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgG ACTACAAGGA CGACGATGAC 2341 AAGCTCGAGa tgcaccgcct gctggcctgg gacgcagcat gcctcccgcc gccgcccgcc 2401 gcctttagac ccatggaagt ggccaacttc tactacgagc ccgactgcct ggcctacggg 2461 gccaaggcgg cccgcgccgc gccgcgcgcc cccgccgccg agccggccat tggcgagcac 2521 gagcgcgcca tcgacttcag cccctacctg gagccgctcg cgcccgccgc ggacttcgcc 2581 gcgcccgcgc ccgcgcacca cgacttcctc tccgacctct tcgccgacga ctacggcgcc 2641 aagccgagca agaagccggc cgactacggt tacgtgagcc tcggccgcgc gggcgccaag 2701 gccgcgccgc ccgcctgctt cccgccgccg cctcccgccg cgctcaaggc ggagccgggc 2761 ttcgaacccg cggactgcaa gcgcgcggac gacgcgcccg ccatggcggc cggtttcccg 2821 ttcgccctgc gcgcctacct gggctaccag gcgacgccga gcggcagcag cggcagcctg 2881 tccacgtcgt cgtcgtccag cccgcccggc acgccgagcc ccgccgacgc caaggccgcg 2941 cccgccgcct gcttcgcggg gccgccggcc gcgcccgcca aggccaaggc caagaagacg 3001 gtggacaagc tgagcgacga gtacaagatg cggcgcgagc gcaacaacat cgcggtgcgc 3061 aagagccgcg acaaggccaa gatgcgcaac ctggagacgc agcacaaggt gctggagctg 3121 acggcggaga acgagcggct gcagaagaag gtggagcagc tgtcgcgaga gctcagcacc 3181 ctgcggaact tgttcaagca gctgcccgag ccgctgctgg cctcggcggg ccactgctag 3241 ggatccCTGC GATATCCAGC gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 3301 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 3361 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 3421 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 3481 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 3541 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 3601 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 3661 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 3721 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 3781 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 3841 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 3901 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 3961 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 4021 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 4081 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 4141 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 4201 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 4261 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 4321 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 4381 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 4441 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 4501 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 4561 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 4621 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 4681 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 4741 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 4801 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 4861 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 4921 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 4981 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 5041 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 5101 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 5161 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 5221 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 5281 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 5341 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 5401 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 5461 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 5521 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 5581 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 5641 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 5701 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 5761 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct 5821 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 5881 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt 5941 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 6001 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 6061 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 6121 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 6181 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 6241 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 6301 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 6361 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 6421 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 6481 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa 6541 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 6601 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 6661 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 6721 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 6781 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 6841 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 6901 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 6961 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 7021 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 7081 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 7141 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 7201 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 7261 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 7321 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 7381 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 7441 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 7501 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 7561 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 7621 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 7681 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 7741 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 7801 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 7861 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 7921 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 7981 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 8041 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 8101 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 8161 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 8221 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 8281 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 8341 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 8401 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 8461 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 8521 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 8581 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 8641 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 8701 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 8761 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 8821 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 8881 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 8941 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca 9001 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 9061 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 9121 aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???