pLV3-CMV-FOXG1-3×FLAG-CopGFP-Puro plasmid

PVT112109

pLV3-CMV-FOXG1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-FOXG1(human)-3×FLAG-CopGFP-Puro
Alias: NM_005249.5;BF1; BF2; FHKL3; FKH2; FKHL1; FKHL2; FKHL3;FKHL4; FOXG1A; FOXG1B; FOXG1C; HBF-1; HBF-2; HBF-3;HBF-G2; HBF2; HFK1; HFK2; HFK3; KHL2; QIN
Gene lenght: 1467
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FOXG1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-FOXG1-3×FLAG-CopGFP-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-FOXG1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgc tggacatggg agataggaaa 2341 gaggtgaaaa tgatccccaa gtcctcgttc agcatcaaca gcctggtgcc cgaggcggtc 2401 cagaacgaca accaccacgc gagccacggc caccacaaca gccaccaccc ccagcaccac 2461 caccaccacc accaccatca ccaccacccg ccgccgcccg ccccgcaacc gccgccgccg 2521 ccgcagcagc agcagccgcc gccgccgccg cccccggcac cgcagccccc ccagacgcgg 2581 ggcgccccgg ccgccgacga cgacaagggc ccccagcagc tgctgctccc gccgccgcca 2641 ccgccaccac cggccgccgc cctggacggg gctaaagcgg acgggctggg cggcaagggc 2701 gagccgggcg gcgggccggg ggagctggcg cccgtcgggc cggacgagaa ggagaagggc 2761 gccggcgccg ggggggagga gaagaagggg gcgggcgagg gcggcaagga cggggagggg 2821 ggcaaggagg gcgagaagaa gaacggcaag tacgagaagc cgccgttcag ctacaacgcg 2881 ctcatcatga tggccatccg gcagagcccc gagaagcggc tcacgctcaa cggcatctac 2941 gagttcatca tgaagaactt cccttactac cgcgagaaca agcagggctg gcagaactcc 3001 atccgccaca atctgtccct caacaagtgc ttcgtgaagg tgccgcgcca ctacgacgac 3061 ccgggcaagg gcaactactg gatgctggac ccgtcgagcg acgacgtgtt catcggcggc 3121 accacgggca agctgcggcg ccgctccacc acctcgcggg ccaagctggc cttcaagcgc 3181 ggtgcgcgcc tcacctccac cggcctcacc ttcatggacc gcgccggctc cctctactgg 3241 cccatgtcgc ccttcctgtc cctgcaccac ccccgcgcca gcagcacttt gagttacaac 3301 ggcaccacgt cggcctaccc cagccacccc atgccctaca gctccgtgtt gactcagaac 3361 tcgctgggca acaaccactc cttctccacc gccaacggcc tgagcgtgga ccggctggtc 3421 aacggggaga tcccgtacgc cacgcaccac ctcacggccg ccgcgctagc cgcctcggtg 3481 ccctgcggcc tgtcggtgcc ctgctctggg acctactccc tcaacccctg ctccgtcaac 3541 ctgctcgcgg gccagaccag ttactttttc ccccacgtcc cgcacccgtc aatgacttcg 3601 cagagcagca cgtccatgag cgccagggcc gcgtcctcct ccacgtcgcc gcaggccccc 3661 tcgaccctgc cctgtgagtc tttaagaccc tctttgccaa gttttacgac gggactgtct 3721 gggggactgt ctgattattt cacacatcaa aatcaggggt cttcttccaa ccctttaata 3781 catggatccG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG 3841 GATGACGATG ACAAGtagcg gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg 3901 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg 3961 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 4021 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 4081 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct 4141 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg 4201 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg 4261 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc 4321 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc 4381 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag 4441 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag 4501 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg 4561 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac 4621 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc 4681 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac 4741 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc 4801 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc 4861 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc 4921 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc 4981 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag 5041 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc 5101 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga 5161 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag 5221 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 5281 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 5341 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 5401 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 5461 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 5521 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 5581 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 5641 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 5701 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 5761 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 5821 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 5881 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 5941 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 6001 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 6061 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 6121 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 6181 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa ATCATCGTCC 6241 TTTCCTtggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 6301 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 6361 cctcttccgc gtctTcgcct tcgccctcag acgagtcgga tctccctttg gGccgcctcc 6421 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 6481 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaGataag atctgctttt 6541 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 6601 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 6661 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 6721 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 6781 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 6841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 6901 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctaTCCCGCC CCTAACTCCG 6961 CCCAtcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 7021 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 7081 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct 7141 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 7201 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 7261 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 7321 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 7381 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 7441 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 7501 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 7561 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 7621 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 7681 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 7741 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 7801 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 7861 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 7921 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 7981 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 8041 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 8101 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 8161 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 8221 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 8281 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 8341 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 8401 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 8461 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 8521 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 8581 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 8641 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 8701 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 8761 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 8821 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 8881 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 8941 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 9001 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 9061 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 9121 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 9181 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 9241 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 9301 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 9361 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 9421 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 9481 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 9541 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 9601 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 9661 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 9721 gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.