pLV3-CMV-GGT6-3×FLAG-mCherry-Puro plasmid

PVT109735

pLV3-CMV-GGT6-3×FLAG-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-GGT6(human)-3×FLAG-mCherry-Puro
Alias: NM_001288702.2
Gene lenght: 1497
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-GGT6-3×FLAG-mCherry-Puro plasmid Description

pLV3-CMV-GGT6-3×FLAG-mCherry-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-GGT6-3×FLAG-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggagcg ggcagaagag cccgtggtct atcagaagct 2341 gctgccctgg gagccaagct tggagtcgga ggaggaagtg gaggaggagg agacatcaga 2401 ggcgctggtt ctaaaccccc ggaggcacca ggactcttcc aggaacaagg ctggcgggct 2461 gcccggaacc tgggcccgtg tagtggcagc cctgctgctg ctggctgttg gctgctccct 2521 ggctgtgagg cagctccaga atcagggcag gtcgacagga agcttgggct ctgtggcccc 2581 tccacccggc ggacactccc acggccctgg cgtataccac cacggtgcca tcatcagccc 2641 tgcagccaca tgctcccacc taggccgaga gctgcttgtt gccgggggca acgtcgtgga 2701 tgctggagtt ggagctgcat tgtgcctggc agtggtgcat cctcatgcca cggggctagg 2761 tgccatgttt tggggcctct tccacgatag ctcctcaggc aattccacgg ccctgacatc 2821 aggcccagca cagaccctgg cccccggcct ggggctgccc gcggctctgc ccaccctgca 2881 cctgctgcat gcacgcttcg gccgcctgcc ctggccacgc ctgctagtgg gccccaccac 2941 gctggctcag gagggcttcc tggtggacac acccctggca agggctctgg tggctcgggg 3001 cacagaaggc ctctgtccac tactttgcca tgctgatggg acacccctgg gcgctggggc 3061 ccgagccacc aacccacaac tggcagctgt gcttcgcagc gcagccctcg ctcccacctc 3121 agaccttgct ggggatgctc tactgagtct actggcggga gacctggggg tggaggtgcc 3181 ctcggctgtg cccaggccca ctttggaacc agcagagcag ctacctgtgc cccagggcat 3241 cctgttcacc acccccagtc cctcagctgg cccagaactg ctggcactgt tggaggcagc 3301 cctgcgctcc ggggcgccca tccctgaccc ctgcccaccg ttcctgcaga ctgctgtgag 3361 ccccgagagc agtgccctgg ccgccgtgga cagcagcggc tctgtgctcc ttctcacctc 3421 ctcgctcaac tgctcctttg gctctgcaca cctgtcccca agcactgggg ttctgctcag 3481 caacctggtg gccaagtcta ccactagtgc ctgggcctgc cccctcatcc tccgtggcag 3541 cctggatgac acagaggctg atgtgttggg gcttgtggct tcagggaccc ctgatgtggc 3601 cagggccatg actcacaccc tactcaggca tctggcagca aggcccccta cccaggccca 3661 gcaccagcat cagggtcagc aagaaccaac agagcatccc agcacttgtg gccaagggac 3721 cctgctccag gtggcagccc acacagagca cgcccatgtc tccagtgtcc cccatgcctg 3781 ctgccccttc caggggttcg gatccGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA 3841 TGACATCGAC TACAAGGATG ACGATGACAA Gtagcggccg cGaaggatct gcgatcgctc 3901 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 3961 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 4021 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 4081 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 4141 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 4201 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 4261 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 4321 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 4381 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac 4441 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa 4501 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg 4561 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc 4621 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca 4681 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg 4741 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga 4801 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt 4861 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg 4921 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc 4981 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt 5041 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga 5101 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg gatctcgcga 5161 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga 5221 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct 5281 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat 5341 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 5401 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 5461 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 5521 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 5581 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 5641 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 5701 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 5761 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc 5821 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 5881 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 5941 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 6001 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 6061 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 6121 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 6181 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 6241 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 6301 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg 6361 ccctcagacg agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat 6421 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 6481 gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt 6541 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 6601 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 6661 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 6721 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 6781 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 6841 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 6901 tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc 6961 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 7021 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat 7081 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 7141 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 7201 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 7261 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 7321 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 7381 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 7441 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 7501 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7561 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 7621 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 7681 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 7741 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 7801 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 7861 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 7921 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 7981 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 8041 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 8101 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 8161 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 8221 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 8281 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 8341 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 8401 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 8461 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 8521 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 8581 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 8641 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 8701 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 8761 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 8821 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 8881 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 8941 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 9001 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 9061 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 9121 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 9181 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 9241 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 9301 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 9361 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 9421 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt 9481 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac 9541 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg 9601 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg 9661 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.